Overexpression of Ets-1 in human hematopoietic progenitor cells blocks erythroid and promotes megakaryocytic differentiation

Cell Death and Differentiation

Volumn 13, Issue 7, 2006, Pages 1064-1074

  Lulli, V ^a   Romania, P ^a   Morsilli, O ^a   Gabbianelli, M ^a   Pagliuca, A ^a   Mazzeo, S ^a   Testa, U ^a   Peschle, C ^a,b   Marziali, G ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

Ets binding sites; GATA 2; Nuclear export signal; Subcellular localization; Transcription factors

Indexed keywords

CD34 ANTIGEN; ERYTHROPOIETIN RECEPTOR; LEUCINE; TRANSCRIPTION FACTOR ETS 1; TRANSCRIPTION FACTOR GATA 1; TRANSCRIPTION FACTOR GATA 2;

ADULT; ARTICLE; CARCINOGENESIS; CELL CULTURE; CELL DIFFERENTIATION; CELL MATURATION; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; DEVELOPMENTAL BIOLOGY; DOWN REGULATION; ERYTHROID CELL; GENE OVEREXPRESSION; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; INTRACELLULAR TRANSPORT; MEGAKARYOCYTE; MEGAKARYOPOIESIS; NUCLEAR EXPORT SIGNAL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; UPREGULATION;

ACTIVE TRANSPORT, CELL NUCLEUS; ADULT; ANIMALS; ANTIGENS, CD34; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELL LINE; CELL NUCLEUS; CELLS, CULTURED; ERYTHROID CELLS; GATA1 TRANSCRIPTION FACTOR; GATA2 TRANSCRIPTION FACTOR; GENE EXPRESSION; HEMATOPOIETIC STEM CELLS; HUMANS; MEGAKARYOCYTES; MICE; MICROSCOPY, CONFOCAL; NUCLEAR EXPORT SIGNALS; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTO-ONCOGENE PROTEIN C-ETS-1; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 33745212655     PISSN: 13509047     EISSN: 14765403     Source Type: Journal    
DOI: 10.1038/sj.cdd.4401811     Document Type: Article

References (40)

1. Oikawa T and Yamada T (2003) Molecular biology of the Ets family of transcription factors. Gene 303: 114
2. Papas TS, Bhat NK, Spyropoulos DD, Mjaatvedt AE, Voumakis J, Seth A and Watson DK (1997) Functional relationships among ETS gene family members. Leukemia 11: 557-566
3. Watson DK and Seth A (2000) Ets Gene Family. Oncogene Rev. 19 (55) review issue 5, Macmillan Publishers Ltd
4. Hahn SL and Wasylyk B (1994) The oncoprotein v-ETS is less selective in DNA binding than c-ETS-1 due to the C-terminal sequence change. Oncogene 9: 2499-2512
5. Cowley DO and Graves BJ (2000) Phosphorylation represses Ets-1 DNA binding by reinforcing autoinhibition. Genes Dev. 14: 366-376
6. Seidel JJ and Graves BJ (2002) An ERK2 docking site in the Pointed domain distinguishes a subset of ETS transcription factors. Genes Dev. 16: 127-137
7. Lelievre E, Lionneton F, Soncin F and Vandenbunder B (2001) The Ets family contains transcriptional activators and repressors involved in angiogenesis. Int. J. Biochem. Cell Biol. 4: 391-407
8. Bories JC, Willerford DM, Grevin D, Davidson L, Camus A, Martin P, Stehelin D and Alt FW (1995) Increased T-cell apoptosis and terminal B-cell differentiation induced by inactivation of the Ets-1 proto-oncogene. Nature 377: 635-638
9. Barton K, Muthusamy N, Fischer C, Ting CN, Walunas TL, Lanier LL and Leiden JM (1998) The Ets-1 transcription factor is required for the development of natural killer cells in mice. Immunity 9: 555-563
10. Sieweke MH, Tekotte H, Frampton J and Graf T (1996) MafB is an interaction partner and repressor of Ets-1 that inhibits erythroid differentiation. Cell 85: 49-60
11. Clausen PA, Athanasiou M, Chen Z, Dunn KJ, Zhang Q, Lautenberger JA, Mavrothalassitis G and Blair DG (1997) Ets-1 induces increased expression of erythroid markers in the pluripotent erythroleukemic cell lines K562 and HEL. Leukemia 11: 1224-1233
12. Kraut N, Frampton J, McNagny KM and Graf T (1994) A functional ETS DNA-binding domain is required to maintain multipotency of hematopoietic progenitors transformed by Myb-Ets. Genes Dev. 8: 33-44
13. Rossi F, McNagny KM, Logie C, Stewart AF and Graf T (1996) Excision of Ets by an inducible site-specific recombinase causes differentiation of Myb-Ets-transformed hematopoietic progenitors. Curr. Biol. 6: 866-872
14. Teuri K, Takashi Y, Kitazawa J, Toki T, Yokoyama M and Ito E (2000) Expression of transcription factors during megakaryocytic differentiation of CD34+ cells from human cord blood induced by thrombopoietin. Tohoku. J. Exp. Med. 192: 259-273
15. Shivdasani RA (2001) Molecular and transcriptional regulation of megakaryocyte differentiation. Stem Cell 19: 397-407
16. Tsai FY, Keller G, Kuo FC, Weiss M, Chen J, Rosenblatt M, Alt FW and Orkin SH (1994) An early hematopoietic defect in mice lacking the transcription factor GATA-2. Nature 371: 221-226
17. Tsai FY and Orkin SH (1997) Transcription factor GATA-2 is required for proliferation/survival of early hematopoietic cells and mast cell formation, but not for erythroid and myeloid terminal differentiation. Blood 89: 3636-3643
18. Shivdasani RA and Orkin SH (1996) The transcriptional control of hematopoiesis. Blood 87: 4025-4039
19. Ikonomi P, Rivera CE, Riordan M, Washington G, Schechter AN and Noguchi CT (2000) Overexpression of GATA-2 inhibits erythroid and promotes megakaryocyte differentiation. Exp. Hematology 28: 1423-1431
20. Kamesaki H, Michaud GY, Irving SG, Suwabe N, Kamesaki S, Okuma M and Cossman J (1996) TPA-induced arrest of erythroid differentiation is coupled with downregulation of GATA-1 and upregulation of GATA-2 in an erythroid cell line SAM-1. Blood 87: 999-1005
21. Ohneda K and Yamamoto M (2002) Roles of hematopoietic transcription factors GATA-1 and GATA-2 in the development of red blood cell lineage. Acta Haematol. 108: 237-245
22. Visvader JE, Crossley M, Hill J, Orkin SH and Adams JM (1995) The C-terminal zinc finger of GATA-1 or GATA-2 is sufficient to induce megakaryocytic differentiation of an early myeloid cell line. Mol. Cell. Biol. 15: 634-641
23. Marziali G, Perrotti E, Ilari R, Lulli V, Coccia EM, Moret R, Kuhn LC, Testa U and Battistini A (2002) Role of Ets-1 in transcriptional regulation of transferrin receptor and erythroid differentiation. Oncogene 21: 7933-7944
24. Mattaj IW and Englmeier L (1998) Nucleocytoplasmic transport: The soluble phase. Annu. Rev. Biochem. 67: 265-306
25. Ducret C, Maira SM, Dierich A and Wasylyk B (1999) The Net repressor is regulated by nuclear export in response to anisomycin, UV, and heat shock. Mol. Cell. Biol. 19: 7076-7087
26. Wood LD, Irvin BJ, Nucifora G, Luce KS and Hiebert SW (2003) Small ubiquitin-like modifier conjugation regulates nuclear export of TEL, a putative tumor suppressor. Proc. Natl. Acad. Sci. 100: 3257-3262
27. Fleenor DE, Langdon SC, deCastro CM and Kaufman RE (1996) Comparison of human and Xenopus GATA-2 promoters. Gene 179: 219-223
28. Kola I, Brookes S, Green AR, Garber R, Tymms M, Papas TS and Seth A (1993) The ETS-1 transcription factor is widely expressed during murine embryo development and is associated with mesodermal cells involved in morphogenetic processes such as organ formation. Proc. Natl. Acad. Sci. 90: 7588-7592
29. Koury MJ, Sawyer ST and Brandt SJ (2002) New insights into erythropoiesis. Curr. Opin. Hematol. 9: 93-100
30. Imagawa S, Yamamoto M and Miura Y (1997) Negative regulation of the erythropoietin gene expression by the GATA transcription factors. Blood 4: 1430-1439
31. Deveaux S, Filipe A, Lemarchandel V, Ghysdael J, Romeo PH and Mignotte V (1996) Analysis of the thrombopoietin receptor (MPL) promoter implicates GATA and Ets proteins in the coregulation of megakaryocyte-specific genes. Blood 87: 4678-4685
32. Minami T, Tachibana K, Imanishi T and Doi T (1998) Both Ets-1 and GATA-1 are essential for positive regulation of platelet factor 4 gene expression. Eur. J. Biochem. 258: 879-889
33. Lemarchandel V, Ghysdael J, Mignotte V, Rahuel C and Romeo PH (1993) GATA and Ets cis-acting sequences mediate megakaryocyte-specific expression. Mol. Cell. Biol. 13: 668-676
34. Eisbacher M, Holmes ML, Newton A, Hogg PJ, Khachigian LM, Crossley M and Chong BH (2003) Protein-protein interaction between Fli-1 and GATA-1 mediates synergistic expression of megakaryocyte-specific genes through cooperative DNA binding. Mol. Cell. Biol. 23: 3427-3441
35. Pereira R, Quang CT, Lesault I, Dolznig H, Beug H and Ghysdael J (1999) FLI-1 inhibits differentiation and induces proliferation of primary erythroblasts. Oncogene 18: 1597-1608
36. Starck J, Doubeikovski A, Sarrazin S, Gonnet C, Rao G, Skoultchi A, Godet J, Dusanter-Fourt I and Morle F (1999) Spi-1/PU.1 is a positive regulator of the Fli-1 gene involved in inhibition of erythroid differentiation in Friend Erythroleukemic cell lines. Mol Cell Biol. 19: 121-135
37. Bhat NK, Fisher RJ, Fujiwara S, Ascione R and Papas TS (1987) Temporal and tissue-specific expression of mouse ets genes. Proc. Natl. Acad. Sci. 84: 3161-3165
38. Breton-Gorius, J, Favier R, Guichard J, Cherif D, Berger R, Debili N, Vainchenker W and Douay L (1995) A new congenital dysmegakaryopoietic thrombocytopenia (Paris-Trousseau) associated with giant platelet alpha-granules and chromosome 11 deletion at 11q23. Blood 85: 1805-1814
39. Coppola S, Narciso L, Feccia T, Bonci D, Calabrò L, Morsilli O, Gabbianelli M, De Maria R, Testa U and Peschle C (2005) Enforced expression of KDR receptor promotes proliferation, survival and megakaryocytic differentiation of TF1 progenitor cell line. Cell Death Differentiation X: 1-14
40. Kilstrup-Nielsen C, Alessio M and Zappavigna V (2003) PBX1 nuclear export is regulated independently of PBX-MEINOX interaction by PKA phosphorylation of the PBC-B domain. EMBO J. 22: 89-99