Immunodominant epitope in the C-terminus of a variable major protein in Borrelia duttonii, an agent of tick-borne relapsing fever

Microbiology and Immunology

Volumn 50, Issue 4, 2006, Pages 293-305

  Tabuchi, Norihiko ^a   Tomoda, Koichiro ^a   Kawaguchi, Hiroshi ^a   Iwamoto, Hiroyuki ^a   Fukunaga, Masahito ^a  

^a FUKUYAMA UNIVERSITY   (Japan)

Author keywords

Borrelia duttonii; Epitope; Infection; Variable major protein

Indexed keywords

BACTERIAL ANTIGEN; EPITOPE; FREUND ADJUVANT; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; RECOMBINANT VACCINE; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG; VARIABLE MAJOR PROTEIN P; HYBRID PROTEIN; LIPOPROTEIN; OUTER MEMBRANE PROTEIN; PEPTIDE FRAGMENT; VMP PROTEIN, BORRELIA;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIGEN ANTIBODY REACTION; ANTIGEN PURIFICATION; ANTIGENIC VARIATION; ANTIGENICITY; ARTICLE; BORRELIA; BORRELIA DUTTONII; BORRELIA INFECTION; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOREACTIVITY; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DOMAIN; RABBIT; TICK BORNE DISEASE; WESTERN BLOTTING; CHEMICAL STRUCTURE; FEMALE; GENETICS; HUMAN; IMMUNOLOGY; MICROBIOLOGY; MOLECULAR GENETICS; PRESCHOOL CHILD; SEQUENCE ALIGNMENT; TANZANIA;

BORRELIA DUTTONII; IXODIDA;

AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; BORRELIA; CHILD, PRESCHOOL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; IMMUNODOMINANT EPITOPES; LIPOPROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; RECOMBINANT FUSION PROTEINS; RELAPSING FEVER; SEQUENCE ALIGNMENT; TANZANIA;

EID: 33745176271     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2006.tb03797.x     Document Type: Article

References (45)

1. Barbour, A.G. 1990. Antigenic variation of a relapsing fever Borrelia species. Annu. Rev. Microbiol. 44: 155-171.
2. Barbour, A.G., and Restrepo, B.I. 2000. Antigenic variation in vector-borne pathogens. Emerg. Infect. Dis. 6: 449-457.
3. Barstad, P.A., Coligan, J.E., Raum, M.G., and Barbour, A.G. 1985. Variable major proteins of Borrelia hermsii. Epitope mapping and partial sequence analysis of CNBr peptides. J. Exp. Med. 161: 1302-1314.
4. Becker, M., Bunikis, J., Lade, B.D., Dunn, J.J., Barbour, A.G., and Lawson, C.L. 2005. Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target. J. Biol. Chem. 280: 17363-17370.
5. Brown, E.L., Kim, J.H., Reisenbichler, E.S., and Höök, M. 2005. Multicomponent Lyme vaccine: three is not a crowd. Vaccine 23: 3687-3696.
6. Burman, N., Bergström, S., Restrepo, B.I., and Barbour, A.G. 1990. The variable antigens Vmp7 and Vmp21 of the relapsing fever bacterium Borrelia hermsii are structurally analogous to the VSG proteins of the African trypanosome. Mol. Microbiol. 4: 1715-1726.
7. Casjens, S., Palmer, N., van Vugt, R., Huang, W.M., Stevenson, B., Rosa, P., Lathigra, R., Sutton, G., Peterson, J., Dodson, R.J., Haft, D., Hickey, E., Gwinn, M., White, O., and Fraser, C.M. 2000. A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi. Mol. Microbiol. 35: 490-516.
8. Coleman, J.L., Rogers, R.C., Rosa, P.A., and Benach, J.L. 1994. Variations in the ospB gene of Borrelia burgdorferi result in differences in monoclonal antibody reactivity and in production of escape variants. Infect. Immun. 62: 303-307.
9. Cutler, S.J., Akintunde, C.O., Moss, J., Fukunaga, M., Kurtenbach, K., Talbert, A., Zhang, H., Wright, D.J., and Warrell, D.A. 1999. Successful in vitro cultivation of Borrelia duttonii and its comparison with Borrelia recurrentis. Int. J. Syst. Bacteriol. 49: 1793-1799.
10. Eicken, C., Sharma, V., Klabunde, T., Lawrenz, M.B., Hardham, J.M., Norris, S.J., and Sacchettini, J.C. 2002. Crystal structure of Lyme disease variable surface antigen VlsE of Borrelia burgdorferi. J. Biol. Chem. 277: 21691-21696.
11. Eicken, C., Sharma, V., Klabunde, T., Owens, R.T., Pikas, D.S., Höök, M., and Sacchettini, J.C. 2001. Crystal structure of Lyme disease antigen outer surface protein C from Borrelia burgdorferi. J. Biol. Chem. 276: 10010-10015.
12. Escudero, R., Halluska, M.L., Backenson, P.B., Coleman, J.L., and Benach, J.L. 1997. Characterization of the physiological requirements for the bactericidal effects of a monoclonal antibody to OspB of Borrelia burgdorferi by confocal microscopy. Infect. Immun. 65: 1908-1915.
13. Fikrig, E., Tao, H., Barthold, S.W., and Flavell, R.A. 1995. Selection of variant Borrelia burgdorferi isolates from mice immunized with outer surface protein A or B. Infect. Immun. 63: 1658-1662.
14. Fikrig, E., Tao, H., Kantor, F.S., Barthold, S.W., and Flavell, R.A. 1993. Evasion of protective immunity by Borrelia burgdorferi by truncation of outer surface protein B. Proc. Natl. Acad. Sci. U.S.A. 90: 4092-4096.
15. Fraser, C.M., Casjens, S., Huang, W.M., Sutton, G.G., Clayton, R., Lathigra, R., White, O., Ketchum, K.A., Dodson, R., Hickey, E.K., Gwinn, M., Dougherty, B., Tomb, J.F., Fleischmann, R.D., Richardson, D., Peterson, J., Kerlavage, A.R., Quackenbush, J., Salzberg, S., Hanson, M., van Vugt, R., Palmer, N., Adams, M.D., Gocayne, J., Weidman, J., Utterback, T., Watthey, L., McDonald, L., Artiach, P., Bowman, C., Garland, S., Fujii, C., Cotton, M.D., Horst, K., Roberts, K., Hatch, B., Smith, H.O., and Venter, J.C. 1997. Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390: 580-586.
16. Golde, W.T., Piesman, J., Dolan, M.C., Kramer, M., Hauser, P., Lobet, Y., Capiau, C., Desmons, P., Voet, P., Dearwester, D., and Frantz, J.C. 1997. Reactivity with a specific epitope of outer surface protein A predicts protection from infection with the Lyme disease spirochete, Borrelia burgdorferi. Infect. Immun. 65: 882-889.
17. Guan, K.L., and Dixon, J.E. 1991. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal. Biochem. 192: 262-267.
18. Guex, N., and Peitsch, M.C. 1997. SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18: 2714-2723.
19. Hopp, T.P., and Woods, K.R. 1981. Prediction of protein antigenic determinants from amino acid sequences. Proc. Natl. Acad. Sci. U.S.A. 78: 3824-3828.
20. Johnson, B.J., Sviat, S.L., Happ, C.M., Dunn, J.J., Frantz, J.C., Mayer, L.W., and Piesman, J. 1995. Incomplete protection of hamsters vaccinated with unlipidated OspA from Borrelia burgdorferi infection is associated with low levels of antibody to an epitope defined by mAb LA-2. Vaccine 13: 1086-1094.
21. Kawabata, H., Myouga, F., Inagaki, Y., Murai, N., and Watanabe, H. 1998. Genetic and immunological analyses of Vls (VMP-like sequences) of Borrelia burgdorferi. Microb. Pathog. 24: 155-166.
22. Kitten, T., and Barbour, A.G. 1990. Juxtaposition of expressed variable antigen genes with a conserved telomere in the bacterium Borrelia hermsii. Proc. Natl. Acad. Sci. U.S.A. 87: 6077-6081.
23. Koide, S., Yang, X., Huang, X., Dunn, J.J., and Luft, B.J. 2005. Structure-based design of a second-generation Lyme disease vaccine based on a C-terminal fragment of Borrelia burgdorferi OspA. J. Mol. Biol. 350: 290-299.
24. Kumaran, D., Eswaramoorthy, S., Luft, B.J., Koide, S., Dunn, J.J., Lawson, C.L., and Swaminathan, S. 2001. Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi. EMBO J. 20: 971-978.
25. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
26. Li, H., Dunn, J.J., Luft, B.J., and Lawson, C.L. 1997. Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab. Proc. Natl. Acad. Sci. U.S.A. 94: 3584-3589.
27. Mathiesen, M.J., Christiansen, M., Hansen, K., Holm, A., Åsbrink, E., and Theisen, M. 1998. Peptide-based OspC enzyme-linked immunosorbent assay for serodiagnosis of Lyme borreliosis. J. Clin. Microbiol. 36: 3474-3479.
28. Mathiesen, M.J., Holm, A., Christiansen, M., Blom, J., Hansen, K., Østergaard, S., and Theisen, M. 1998. The dominant epitope of Borrelia garinii outer surface protein C recognized by sera from patients with neuroborreliosis has a surface-exposed conserved structural motif. Infect. Immun. 66: 4073-4079.
29. Meier, J.T., Simon, M.I., and Barbour, A.G. 1985. Antigenic variation is associated with DNA rearrangements in a relapsing fever Borrelia. Cell 41: 403-409.
30. Pal, U., de Silva, A.M., Montgomery, R.R., Fish, D., Anguita, J., Anderson, J. F., Lobet, Y., and Fikrig, E. 2000. Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A. J. Clin. Investig. 106: 561-569.
31. Pal, U., Li, X., Wang, T., Montgomery, R.R., Ramamoorthi, N., Desilva, A.M., Bao, F., Yang, X., Pypaert, M., Pradhan, D., Kantor, F.S., Telford, S., Anderson, J.F., and Fikrig, E. 2004. TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi. Cell 119: 457-468.
32. Peitsch, M.C. 1995. Protein modelling by E-mail. Bio/Technology 13: 658-660.
33. Plasterk, R.H., Simon, M.I., and Barbour, A.G. 1985. Transposition of structural genes to an expression sequence on a linear plasmid causes antigenic variation in the bacterium Borrelia hermsii. Nature 318: 257-263.
34. Restrepo, B.I., Kitten, T., Carter, C.J., Infante, D., and Barbour, A.G. 1992. Subtelomeric expression regions of Borrelia hermsii linear plasmids are highly polymorphic. Mol. Microbiol. 6: 3299-3311.
35. Sears, J.E., Fikrig, E., Nakagawa, T.Y., Deponte, K., Marcantonio, N., Kantor, F.S., and Flavell, R.A. 1991. Molecular mapping of Osp-A mediated immunity against Borrelia burgdorferi, the agent of Lyme disease. J. Immunol. 147: 1995-2000.
36. Schubach, W.H., Mudri, S., Dattwyler, R.J., and Luft, B.J. 1991. Mapping antibody-binding domains of the major outer surface membrane protein (OspA) of Borrelia burgdorferi. Infect. Immun. 59: 1911-1915.
37. Schwan, T.G. 2003. Temporal regulation of outer surface proteins of the Lyme-disease spirochaete Borrelia burgdorferi. Biochem. Soc. Trans. 31: 108-112.
38. Schwede, T., Kopp, J., Guex, N., and Peitsch, M.C. 2003. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res. 31: 3381-3385.
39. Steere, A.C., Sikand, V.K., Meurice, F., Parenti, D.L., Fikrig, E., Schoen, R.T., Nowakowski, J., Schmid, C.H., Laukamp, S., Buscarino, C., Krause, D.S., and the Lyme Disease Vaccine Study Group. 1998. Vaccination against Lyme disease with recombinant Borrelia burgdorferi outer-surface lipoprotein A with adjuvant. N. Engl. J. Med. 339: 209-215.
40. Tabuchi, N., Mitani, H., Seino, S., and Fukunaga, M. 2002. The 44-kb linear plasmid molecule in the relapsing fever agent Borrelia duttonii strain Ly serve as a preservation of vmp genes. Microbiol. Immunol. 46: 159-165.
41. Takahashi, Y., Cutler, S.J., and Fukunaga, M. 2000. Size conversion of a linear plasmid in the relapsing fever agent Borrelia duttonii. Microbiol. Immunol. 44: 1071-1074.
42. Talbert, A., Nyange, A., and Molteni, F. 1998. Spraying tick-infested houses with lambda-cyhalothrin reduces the incidence of tick-borne relapsing fever in children under five years old. Trans. R. Soc. Trop. Med. Hyg. 92: 251-253.
43. Towbin, H., Staehelin, T., and Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354.
44. Wallich, R., Jahraus, O., Stehle, T., Tran, T.T., Brenner, C., Hofmann, H., Gern, L., and Simon, M.M. 2003. Artificial-infection protocols allow immunodetection of novel Borrelia burgdorferi antigens suitable as vaccine candidates against Lyme disease. Eur. J. Immunol. 33: 708-719.
45. Zhang, J.R., Hardham, J.M., Barbour, A.G., and Norris, S.J. 1997. Antigenic variation in Lyme disease borreliae by promiscuous recombination of VMP-like sequence cassettes. Cell 89: 275-285.