The antifibrogenic effect of (-)-epigallocatechin gallate results from the induction of de novo synthesis of glutathione in passaged rat hepatic stellate cells

Laboratory Investigation

Volumn 86, Issue 7, 2006, Pages 697-709

  Yumei, Fu ^a   Zhou, Yajun ^a   Zheng, Shizhong ^a   Chen, Anping ^a  

^a LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

Author keywords

Antioxidant; Fibrogenesis; Gene expression; Green tea; Oxidative stress; Receptors

Indexed keywords

EPIGALLOCATECHIN GALLATE; GLUTATHIONE; GREEN TEA EXTRACT; PROCOLLAGEN; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 1; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 2;

ANIMAL CELL; ARTICLE; COLLAGEN SYNTHESIS; CONTROLLED STUDY; DRUG MECHANISM; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; LIVER CELL; LIVER FIBROSIS; MALE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN INDUCTION; RAT; SIGNAL TRANSDUCTION; STELLATE CELL;

ANIMALS; ANTIOXIDANTS; CATECHIN; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CULTURE MEDIA; DOSE-RESPONSE RELATIONSHIP, DRUG; EXTRACELLULAR MATRIX; FIBRINOGEN; FIBROSIS; GLUTATHIONE; HEPATOCYTES; IMMEDIATE-EARLY PROTEINS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LIPID PEROXIDATION; LIVER; MALE; PROCOLLAGEN; RATS; RATS, SPRAGUE-DAWLEY; REACTIVE OXYGEN SPECIES; RECEPTORS, TRANSFORMING GROWTH FACTOR BETA; SIGNAL TRANSDUCTION; TEA; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA1;

EID: 33745149328     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.3700425     Document Type: Article

References (43)

1. Friedman SL. Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury. J Biol Chem 2000;275:2247-2250.
2. Friedman SL. Seminars in medicine of the Beth Israel Hospital, Boston. The cellular basis of hepatic fibrosis. Mechanisms and treatment strategies. N Engl J Med 1993;328:1828-1835.
3. Gressner AM, Weiskirchen R, Breitkopf K, et al. Roles of TGF-beta in hepatic fibrosis. Front Biosci 2002;7:d793-d807.
4. Bachem MG, Meyer D, Melchior R, et al. Activation of rat liver perisinusoidal lipocytes by transforming growth factors derived from myofibroblastlike cells. A potential mechanism of self-perpetuation in liver fibrogenesis. J Clin Invest 1992;89:19-27.
5. Bradham DM, Igarashi A, Potter RL, et al. Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10. J Cell Biol 1991;114:1285-1294.
6. Rachfal AW, Brigstock DR. Connective tissue growth factor (CTGF/CCN2) in hepatic fibrosis. Hepatol Res 2003;26:1-9.
7. Fu M, Zhang J, Zhu X, et al. Peroxisome proliferator-activated receptor gamma inhibits transforming growth factor beta-induced connective tissue growth factor expression in human aortic smooth muscle cells by interfering with Smad3. J Biol Chem 2001;276:45888-45894.
8. Leask A, Holmes A, Black CM, et al. Connective tissue growth factor gene regulation. Requirements for its induction by transforming growth factor-beta 2 in fibroblasts. J Biol Chem 2003;278:13008-13015.
9. Paradis V, Dargere D, Vidaud M, et al. Expression of connective tissue growth factor in experimental rat and human liver fibrosis. Hepatology 1999;30:968-976.
10. Paradis V, Dargere D, Bonvoust F, et al. Effects and regulation of connective tissue growth factor on hepatic stellate cells. Lab Invest 2002;82:767-774.
11. Williams EJ, Gaca MD, Brigstock DR, et al. Increased expression of connective tissue growth factor in fibrotic human liver and in activated hepatic stellate cells. J Hepatol 2000;32:754-761.
12. Fridovich I. The biology of oxygen radicals. Science 1978;201:875-880.
13. Halliwell B. Drug antioxidant effects. A basis for drug selection? Drugs 1991;42:569-605.
14. Zima T, Fialova L, Mestek O, et al. Oxidative stress, metabolism of ethanol and alcohol-related diseases. J Biomed Sci 2001;8:59-70.
15. Wu G, Fang YZ, Yang S, et al. Glutathione metabolism and its implications for health. J Nutr 2004;134:489-492.
16. Tsukamoto H. Oxidative stress, antioxidants, and alcoholic liver fibrogenesis. Alcohol 1993;10:465-467.
17. Lee KS, Buck M, Houglum K, et al. Activation of hepatic stellate cells by TGF alpha and collagen type I is mediated by oxidative stress through c-myb expression. J Clin Invest 1995;96:2461-2468.
18. Pietrangelo A, Gualdi R, Casalgrandi G, et al. Molecular and cellular aspects of iron-induced hepatic cirrhosis in rodents. J Clin Invest 1995;95:1824-1831.
19. Ahmad N, Mukhtar H. Green tea polyphenols and cancer: biologic mechanisms and practical implications. Nutr Rev 1999;57:78-83.
20. Rice-Evans C. Implications of the mechanisms of action of tea polyphenols as antioxidants in vitro for chemoprevention in humans. Proc Soc Exp Biol Med 1999;220:262-266.
21. Chen A, Zhang L, Xu J, et al. The antioxidant (-)-epigallocatechin-3- gallate inhibits activated hepatic stellate cell growth and suppresses acetaldehyde-induced gene expression. Biochem J 2002;368:695-704.
22. Schmittgen TD, Zakrajsek BA, Mills AG, et al. Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of end-point and real-time methods. Anal Biochem 2000;285:194-204.
23. Lee DK, Park SH, Yi Y, et al. The hepatitis B virus encoded oncoprotein pX amplifies TGF-beta family signaling through direct interaction with Smad4: potential mechanism of hepatitis B virus-induced liver fibrosis. Genes Dev 2001;15:455-466.
24. Wang G, Gong Y, Anderson J, et al. Antioxidative function of L-FABP in L-FABP stably transfected Chang liver cells. Hepatology 2005;42:871-879.
25. Schmid I, Sakamoto KM. Analysis of DNA content and green fluorescent protein expression. In: Robinson JP, Darzynkiewicz Z, Dean P, Orfao A, Rabinovitch P, Stewart C, Tanke H, Wheeless L (eds). Current Protocols in Cytometry, Vol. 1, John Wiley & Sons: New York, 2001, pp 7.16.1-7.16.10.
26. Dennis PA, Rifkin DB. Cellular activation of latent transforming growth factor beta requires binding to the cation-independent mannose 6-phosphate/insulin-like growth factor type II receptor. Proc Natl Acad Sci USA 1991;88:580-584.
27. Massague J. How cells read TGF-beta signals. Nat Rev Mol Cell Biol 2000;1:169-178.
28. Cotgreave IA. N-acetylcysteine: pharmacological considerations and experimental and clinical applications. Adv Pharmacol 1997;38:205-227.
29. Anderson ME, Luo JL. Glutathione therapy: from prodrugs to genes. Semin Liver Dis 1998;18:415-424.
30. Dooley S, Delvoux B, Lahme B, et al. Modulation of transforming growth factor beta response and signaling during transdifferentiation of rat hepatic stellate cells to myofibroblasts. Hepatology 2000;31:1094-1106.
31. Dooley S, Delvoux B, Streckert M, et al. Transforming growth factor beta signal transduction in hepatic stellate cells via Smad2/3 phosphorylation, a pathway that is abrogated during in vitro progression to myofibroblasts. TGFbeta signal transduction during transdifferentiation of hepatic stellate cells. FEBS Lett 2001;502:4-10.
32. Yata Y, Gotwals P, Koteliansky V, et al. Dose-dependent inhibition of hepatic fibrosis in mice by a TGF-beta soluble receptor: implications for antifibrotic therapy. Hepatology 2002;35:1022-1030.
33. Qi Z, Atsuchi N, Ooshima A, et al. Blockade of type beta transforming growth factor signaling prevents liver fibrosis and dysfunction in the rat. Proc Natl Acad Sci USA 1999;96:2345-2349.
34. George J, Roulot D, Koteliansky VE, et al. In vivo inhibition of rat stellate cell activation by soluble transforming growth factor beta type II receptor: a potential new therapy for hepatic fibrosis. Proc Natl Acad Sci USA 1999;96:12719-12724.
35. Weston BS, Wahab NA, Mason RM. CTGF mediates TGF-beta-induced fibronectin matrix deposition by upregulating active alpha5beta1 integrin in human mesangial cells. J Am Soc Nephrol 2003;14:601-610.
36. Shi-wen X, Pennington D, Holmes A, et al. Autocrine overexpression of CTGF maintains fibrosis: RDA analysis of fibrosis genes in systemic sclerosis. Exp Cell Res 2000;259:213-224.
37. Nieto N, Friedman SL, Greenwel P, et al. CYP2E1-mediated oxidative stress induces collagen type I expression in rat hepatic stellate cells. Hepatology 1999;30:987-996.
38. Nieto N, Friedman SL, Cederbaum AI. Cytochrome P450 2E1-derived reactive oxygen species mediate paracrine stimulation of collagen I protein synthesis by hepatic stellate cells. J Biol Chem 2002;277:9853-9864.
39. Skrzydlewska E, Ostrowska J, Farbiszewski R, et al. Protective effect of green tea against lipid peroxidation in the rat liver, blood serum and the brain. Phytomedicine 2002;9:232-238.
40. Sur-Altiner D, Yenice B. Effect of black tea on lipid peroxidation in carbon tetrachloride treated male rats. Drug Metabol Drug Interact 2000;16:123-128.
41. Guleria RS, Jain A, Tiwari V, et al. Protective effect of green tea extract against the erythrocytic oxidative stress injury during mycobacterium tuberculosis infection in mice. Mol Cell Biochem 2002;236:173-181.
42. Myhrstad MC, Carlsen H, Nordstrom O, et al. Flavonoids increase the intracellular glutathione level by transactivation of the gamma-glutamylcysteine synthetase catalytical subunit promoter. Free Radic Biol Med 2002;32:386-393.
43. Moskaug JO, Carlsen H, Myhrstad MC, et al. Polyphenols and glutathione synthesis regulation. Am J Clin Nutr 2005;81:277S-283S.