Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli

Protein Expression and Purification

Volumn 48, Issue 1, 2006, Pages 104-111

  Király, Orsolya ^a   Guan, Lan ^b   Szepessy, Edit ^a   Tóth, Miklós ^c   Kukor, Zoltán ^c   Sahin Tóth, Miklós ^a  

^a BOSTON UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c SEMMELWEIS UNIVERSITY   (Hungary)

Author keywords

A16V mutation; Aminopeptidase P; Autoactivation; Hereditary pancreatitis; Human cationic trypsinogen; In vitro refolding; Intein; PRSS1

Indexed keywords

AMINOPEPTIDASE; HYBRID PROTEIN; INTEIN; PRSS1 PROTEIN, HUMAN; RECOMBINANT PROTEIN; TRYPSIN; TRYPSINOGEN; X PRO AMINOPEPTIDASE; X-PRO AMINOPEPTIDASE;

ARTICLE; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE DELETION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; PROTEIN PROCESSING;

AMINOPEPTIDASES; BASE SEQUENCE; ENZYME ACTIVATION; ESCHERICHIA COLI; HUMANS; INTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN SPLICING; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SEQUENCE DELETION; TRYPSIN; TRYPSINOGEN;

ESCHERICHIA COLI;

EID: 33646884384     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.01.023     Document Type: Article

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