Affinity purification of recombinant trypsinogen using immobilized ecotin

Protein Expression and Purification

Volumn 12, Issue 2, 1998, Pages 291-294

  Lengyel, Zsolt ^a   Pál, Gábor ^b   Sahin Tóth, Miklós ^a  

^a NAVIX Inc   (United States)

^b EÖTVÖS LORÁND UNIVERSITY   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; ENZYME INHIBITOR; ENZYME PRECURSOR; ESCHERICHIA COLI; IMMOBILIZATION; SERINE PROTEINASE; TRYPSINOGEN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0032029774     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0837     Document Type: Article

References (5)

1. McGrath M. E., Gillmor S. A., Fletterick R. J. Ecotin: Lessons on survival in a protease-filled world. Protein Sci. 4:1995;141-148.
2. Pál G., Szilágyi L., Gráf L. Stable monomeric form of an originally dimeric serine proteinase inhibitor, ecotin, was constructed via site directed mutagenesis. FEBS Lett. 385:1996;165-170.
3. Pál G., Sprengler G., Patthy A., Gráf L. Alteration of the specificity of ecotin, anE. coli. FEBS Lett. 342:1994;57-60.
4. Chung C. H., Ives H. E., Almeda S., Goldberg A. L. Purification fromEscherichia coli. J. Biol. Chem. 258:1983;11032-11038.
5. Gráf L., Craik C. S., Patthy A., Roczniak S., Fletterick R. J., Rutter W. J. Selective alteration of substrate specificity by replacement of aspartic acid-189 with lysine in the binding pocket of trypsin. Biochemistry. 26:1987;2616-2623.