Elevated phosphorylation of Chk1 and decreased phosphorylation of Chk2 are associated with abrogation of G2/M checkpoint control during transformation of Syrian hamster embryo (SHE) cells by Malachite green

Cancer Letters

Volumn 237, Issue 2, 2006, Pages 188-198

  Ashra, Hima ^a   Rao, K V K ^a  

^a Research and Education in Cancer   (India)

Author keywords

Abrogation of G2 M checkpoint; Chk1 and Chk2; DNA damage; Malachite green (MG); Syrian hamster embryo cells

Indexed keywords

CHECKPOINT KINASE 1; CHECKPOINT KINASE 2; CYCLIN B1; CYCLIN DEPENDENT KINASE 1; CYCLOHEXIMIDE; ETHIDIUM BROMIDE; FORMALDEHYDE; MALACHITE GREEN; MIMOSINE; PROTEIN 14 3 3; PROTEIN TYROSINE PHOSPHATASE; REGULATOR PROTEIN;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; ARTICLE; CELL CYCLE ARREST; CELL CYCLE G2 PHASE; CELL CYCLE PHASE; CELL TRANSFORMATION; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA DETERMINATION; EMBRYO; ENZYME PHOSPHORYLATION; FLOW CYTOMETRY; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN STABILITY; SYRIAN HAMSTER; WESTERN BLOTTING;

14-3-3 PROTEINS; ANIMALS; CDC25 PHOSPHATASE; CELL CYCLE; CELL CYCLE PROTEINS; CELL TRANSFORMATION, NEOPLASTIC; COLORING AGENTS; CRICETINAE; CYCLIN B; DNA DAMAGE; MESOCRICETUS; PHOSPHORYLATION; PROTEIN KINASES; PROTEIN-SERINE-THREONINE KINASES; ROSANILINE DYES;

MAMMALIA; MESOCRICETUS AURATUS;

EID: 33646835739     PISSN: 03043835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.canlet.2005.05.044     Document Type: Article

References (38)

1. Carr A. Checkpoints take the next step. Science 271 (1996) 31-315
2. Elledge S.J. Cell cycle checkpoint: preventing an identity crisis. Science 274 (1996) 1664-1672
3. Hartwell L.H., and Weinert T.A. Checkpoints: controls that ensure the order of cell cycle events. Science 246 (1989) 629-634
4. Nurse P. Ordering S phase and M phase in the cell cycle. Cell 79 (1994) 547-550
5. Weinert T. A DNA damage checkpoint meets the cell cycle engine. Science 277 (1997) 1450-1451
6. Lopez-Girona A., Furnari B., Mondesert O., and Russell P. Nuclear localization of Cdc25 is regulated by DNA damage and a 14-3-3 protein. Nature 397 (1999) 172-176
7. Sanchez Y., Wong C., Thoma R.S., Richman R., Wu Z., Piwnica-Worms H., and Elledge S.J. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 277 (1997) 1447-1501
8. Peng Y., Graves P.R., Thoma R.S., Wu Z., Shaw A.S., and Piwnica-Worms H. Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216. Science 277 (1997) 1501-1505
9. Aitken A. 14-3-3 and its possible role in co-ordinating multiple signalling pathways. Trends Cell Biol. 6 (1996) 341-347
10. Kumagai P.S., and Yakowec W.G. Dunphy, 14-3-3 proteins act as negative regulators of the mitotic inducer Cdc25 in Xenopus egg extracts. Mol. Biol. Cell 9 (1998) 345-354
11. Morgan D.O. Cyclin-dependent kinases: engines, clocks, and microprocessors. Annu. Rev. Cell Dev. Biol. 13 (1997) 261-291
12. Gould K.L., and Nurse P. Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. Nature 342 (1989) 39-45
13. Solomon M.J., Lee T., and Kirschner M.W. Role of phosphorylation in p34cdc2 activation: identification of an activating kinase. Mol. Biol. Cell 3 (1992) 13-27
14. Watanbe N., Broome M., and Hunter T. Regulation of the human Wee1Hu CDK tyrosine 15-kinase during the cell cycle. Eur. Mol. Biol. Org. J. 14 (1995) 1878-1891
15. Sebastian B., Kakizuka A., and Hunter T. Cdc25M2 activation of cyclin-dependent kinases by dephosphorylation of threonine-14 and tyrosine-15. Proc. Natl Acad. Sci. USA 90 (1993) 3521-3524
16. Yang J., Bander E.S.G., Moore J.D., Brennan J., Powers M.A., and Kornbluth S. Control of cyclin B1 localization through regulated binding of the nuclear factor CRM1. Genes Dev. 12 (1998) 2131-2143
17. Pines J., and Hunter T. The differential localization of human cyclins A and B is due to a cytoplasmic retention signal in cyclin B. Eur. Mol. Biol. Org. J. 13 (1994) 3772-3781
18. WHO, World Health Organisation. Technical Report Series No. 309. Geneva, Switzerland, 1965.
19. Windholz M., Budhavani S., Bluemetti R.F., and Otterbein E.S. The Merck Index. 10th ed. (1983), Merck and Co., Inc., Rahway, NJ p. 813
20. Rao K.V.K., Mahudawala D.M., and Redkar A.A. Malignant transformation of Syrian hamster embryo (SHE) cells in primary culture by malachite green: transformation is associated with abrogation of G2/M checkpoint control. Cell Biol. Int. 22 (1998) 581-589
21. Panandiker A., Fernandes C., and Rao K.V.K. The cytotoxic properties of malachite green are associated with the increased demethylase, arylhydrocarbon hydroxylase and lipid peroxidation in primary cultures of Syrian hamster embryo cells. Cancer Lett. 67 (1992) 93-101
22. Fernandes C., Lalitha V.S., and Rao K.V.K. Enhancing effects of malachite green on the developmental of hepatic pre-neoplastic lesions induced by N-nitrosodiethylamine in rats. Carcinogenesis 12 (1991) 839-845
23. Rao K.V.K., and Fernandes C. Progressive effects of malachite green at varying concentrations on the development of N-nitrosodiethylamine induced hepatic preneoplastic lesions in rats. Tumori 82 (1996) 280-286
24. Panandiker A., Maru G.B., and Rao K.V.K. Dose effects of malachite green on free radical formation, lipid peroxidation and DNA damage in Syrian hamster embryo cells and their modulation by antioxidants. Carcinogenesis 15 (1994) 2445-2448
25. Klucar J., and AL-Rubeai M. G2 cell cycle arrest and apoptosis are induced in Burkitt's lymphoma cells by the anticancer agent Oracin. Fed. Eur. Biochem. Soc. Lett. 400 (1997) 127-130
26. Singh N.P., McCoy M.T., Tice R.R., and Schneider E.L. A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Res. 175 (1988) 184-191
27. Peterson G.L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83 (1977) 346
28. Towbin H., Staehlin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc. Natl Acad. Sci. USA 76 (1979) 4350-4353
29. Chomczynski P., and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocynate-phenol-chloroform extraction. Anal. Biochem. 162 (1997) 156-159
30. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning-A Laboratory Manual. second ed. (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
31. Krude T. Mimosine arrest proliferating human cells before arrest of DNA replication in a dose dependent manner. Exp. Cell Res. 247 (1999) 148-159
32. Mahudawala D.M., Redkar A.A., Wagh A., Gladstone B., and Rao K.V.K. Malignant transformation of Syrian hamster embryo (SHE) cells in culture by Malachite green-An agent of environmental importance. Ind. J. Exp. Biol. 37 (1999) 904-918
33. Quingua L., Guntuku S., Cui X.S., Matsuoka S., Cortez D., Tamai K., et al. Chk1 is essential kinase that is regulated by Atr and required for G2/M DNA damage checkpoint. Genes Dev. 14 (2000) 1448-1459
34. Girona A.L., Furnari B., Mondesert O., and Russell P. Nuclear localisation of Cdc25 is regulated by DNA damage and a 14-3-3 protein. Nature 397 (1999) 172-175
35. Kumagai A., Yakowec P.S., and Dunphy W.G. 14-3-3 proteins act as negative regulators of the mitotic inducer Cdc25 in Xenopus egg extracts. Mol. Biol. Cell 9 (1998) 345-354
36. Dalal S.N., Schweitzer C.M., Gan J., and DeCaprio J.A. Cytoplasmic localization of human cdc25C during interphase requires an intact 14-3-3 binding site. Mol. Cell Biol. 19 (1999) 4465-4479
37. Muschel R.J., Zhang H.B., and McKenna W.G. Differential effect of ionising radiation on the expression of cyclin A and cyclin B in HeLa cells. Cancer Res. 53 (1993) 1128-1135
38. O'Connor P.M., Ferris D.K., Pagano M., Draetta G., Pines J., Hunter T., Longo D.L., and Kohn K.W. G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently. J. Biol. Chem. 268 (1993) 8298-8308