Theoretical model of restriction endonuclease HpaI in complex with DNA, predicted by fold recognition and validated by site-directed mutagenesis

Proteins: Structure, Function and Genetics

Volumn 63, Issue 4, 2006, Pages 1059-1068

  Skowronek, Krzysztof J ^a   Kosinski, Jan ^a   Bujnicki, Janusz M ^a  

^a INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Poland)

Author keywords

GeneSilico; Model evaluation; ORFan; Remote homology; Threading

Indexed keywords

DNA; RESTRICTION ENDONUCLEASE;

AGAR GEL ELECTROPHORESIS; AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; COMPLEX FORMATION; CRYSTALLOGRAPHY; DNA BINDING; ENZYME ACTIVITY; GENE; MODEL; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PREDICTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; VALIDATION PROCESS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; BINDING SITES; CONSERVED SEQUENCE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DIMERIZATION; DNA; HYDROGEN BONDING; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; REPRODUCIBILITY OF RESULTS; SEQUENCE ALIGNMENT; STRUCTURAL HOMOLOGY, PROTEIN;

EID: 33646808068     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.20920     Document Type: Article

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