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Volumn , Issue 19, 2006, Pages 2012-2014

Synthesis and single enzyme activity of a clicked lipase-BSA hetero-dimer

Author keywords

[No Author keywords available]

Indexed keywords

3 AZIDOPROPYL 1 MALEIMIDE; ASCORBIC ACID; BOVINE SERUM ALBUMIN; CARBOXYFLUORESCEIN; CARRIER PROTEIN; COPPER SULFATE; HETERODIMER; LIPASE B; MALEIMIDE DERIVATIVE; THIOL; UNCLASSIFIED DRUG;

EID: 33646577154     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/b516551b     Document Type: Article
Times cited : (77)

References (31)
  • 23
    • 33646564119 scopus 로고    scopus 로고
    • Mutant TLL (TLL-1823) possessing only one free solvent accessible lysine was provided by Novo enzymes
    • Mutant TLL (TLL-1823) possessing only one free solvent accessible lysine was provided by Novo enzymes
  • 29
    • 33646573712 scopus 로고    scopus 로고
    • 488 renders focusing of the laser beam easier. In this case the labelling occurs selectively on BSA due to the absence of lysines on TLL. In this way the native conformation and catalytic activity of TLL remain intact
    • 488 renders focusing of the laser beam easier. In this case the labelling occurs selectively on BSA due to the absence of lysines on TLL. In this way the native conformation and catalytic activity of TLL remain intact
  • 30
    • 33646560377 scopus 로고    scopus 로고
    • 488 and was immobilized on the hydrophobic glass under otherwise identical conditions. The observed hydrolysis was negligible (see supporting information)
    • 488 and was immobilized on the hydrophobic glass under otherwise identical conditions. The observed hydrolysis was negligible (see supporting information)
  • 31
    • 33646581426 scopus 로고    scopus 로고
    • The initial activity of the dimer on the surface was found to be 80 times higher than in solution. However the substrate concentration in the single enzyme studies was above saturation level, which could not be accomplished in solution
    • The initial activity of the dimer on the surface was found to be 80 times higher than in solution. However the substrate concentration in the single enzyme studies was above saturation level, which could not be accomplished in solution


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.