Single-enzyme kinetics of CALB-catalyzed hydrolysis

Angewandte Chemie - International Edition

Volumn 44, Issue 4, 2005, Pages 560-564

  Velonia, Kelly ^a   Flomenbom, Ophir ^c   Loos, Davey ^b   Masuo, Sadahiro ^b   Cotlet, Mircea ^a   Engelborghs, Yves ^d   Hofkens, Johan ^a   Rowan, Alan E ^a   Klafter, Joseph ^c   Nolte, Roeland J M ^a   De Schryver, Frans C ^b  

^a RADBOUD UNIVERSITY NIJMEGEN   (Netherlands)

^b UNIVERSITY OF LEUVEN   (Belgium)

^c TEL AVIV UNIVERSITY   (Israel)

^d Lab of Chem and Biol Dynamics   (Belgium)

Author keywords

Analytical methods; Enzymes; Fluorescence microscopy; Kinetics; Single molecule studies

Indexed keywords

CONFORMATIONS; ENZYMES; FLUORESCENCE; REACTION KINETICS;

CONFOCAL FLUORESCENCE MICROSCOPY (CFM); SINGLE-ENZYME HYDROLYSIS; SUBSTRATE KINETICS;

HYDROLYSIS;

TRIACYLGLYCEROL LIPASE;

ARTICLE; CANDIDA ANTARCTICA; CATALYSIS; CATALYST; CHEMICAL REACTION; CONCENTRATION RESPONSE; CONFOCAL MICROSCOPY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; FLUORESCENCE MICROSCOPY; HYDROLYSIS; MEASUREMENT; MOLECULAR DYNAMICS; NONHUMAN; PARAMETER; REACTION ANALYSIS; TECHNIQUE;

CANDIDA; CATALYSIS; HYDROLYSIS; KINETICS; LIPASE;

EID: 19944433435     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200460625     Document Type: Article

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