A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy

Nature Biotechnology

Volumn 24, Issue 5, 2006, Pages 577-581

  Kogure, Takako ^a   Karasawa, Satoshi ^a,b,c   Araki, Toshio ^a,b,c   Saito, Kenta ^d   Kinjo, Masataka ^d   Miyawaki, Atsushi ^a  

^a RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

^b Amalgaam Co Ltd   (Japan)

^c Medical & Biological Laboratories Co Ltd   (Japan)

^d HOKKAIDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CORRELATION DETECTORS; FLUORESCENCE; LASERS; MONOMERS; PHARMACODYNAMICS; WAVELENGTH DISPERSIVE SPECTROSCOPY;

FLUORESCENCE CROSS-CORRELATION SPECTROSCOPY (FCCS); MONOMERIC VERSION; PROTEIN EMISSIONS; PROTEIN-PROTEIN INTERACTIONS; WAVELENGTH EXCITATION;

PROTEINS;

CALMODULIN; CASPASE 3; ENZYME; KEIMA PROTEIN; PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; COLOR; CONTROLLED STUDY; CORAL; DUAL COLOR SINGLE LASER FLUORESCENCE CROSS CORRELATION SPECTROSCOPY; FLUORESCENCE; HUMAN; HUMAN CELL; LASER; LIGHT ABSORPTION; MEASUREMENT; MONTIPORA; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; PROTEIN VARIANT; SENSITIVITY AND SPECIFICITY; SPECTROSCOPY; SPECTRUM;

ANIMALS; ANTHOZOA; CALMODULIN; FLUORESCENT DYES; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; ULTRACENTRIFUGATION;

ANTHOZOA; MONTIPORA; SCLERACTINIA;

EID: 33646572554     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1207     Document Type: Article

References (30)

1. Kettling, U., Koltermann, A., Schwille, P. & Eigen, M. Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy. Proc. Natl. Acad. Sci. USA 95, 1416-1420 (1998).
2. Weidemann, T., Wachsmuth, M., Tewes, M., Rippe, K. & Langowski, J. Analysis of ligand binding by two-colour fluorescence cross-correlation spectroscopy. Single Mol. 3, 49-61 (2002).
3. Kim, S.A. & Schwille, P. Intracellular applications of fluorescence correlation spectroscopy: prospects for neuroscience. Curr. Opin. Neurobiol. 13, 583-590 (2003).
4. Saito, K., Wada, I., Tamura, M. & Kinjo, M. Direct detection of caspase-3 activation in single live cells by cross-correlation analysis. Biochem. Biophys. Res. Commun. 324, 849-854 (2004).
5. Kohl, T., Haustein, E. & Schwille, P. Determining protease activity in vivo by fluorescence cross-correlation analysis. Biophys. J. 89, 2770-2782 (2005).
6. Hwang, L.C. & Wohland, T. Single wavelength excitation fluorescence cross-correlation spectroscopy with spectrally similar fluorophores: Resolution for binding studies. J. Chem. Phys. 122, 114708 (1-11) (2005).
7. Martin, B.R., Giepmans, B.N., Adams, S.R. & Tsien, R.Y. Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity. Nat. Biotechnol. 23, 1308-1314 (2005).
8. Helmchen, F. & Denk, W. New developments in multiphoton microscopy. Curr. Opin. Neurobiol. 12, 593-601 (2002).
9. Heinze, K.G., Koltermann, A. & Schwille, P. Simultaneous two-photon excitation of distinct labels for dual-color fluorescence crosscorrelation analysis. Proc. Natl. Acad. Sci. USA 97, 10377-10382 (2000).
10. Kohl, T., Heinze, K.G., Kuhlemann, R., Koltermann, A. & Schwille, P. A protease assay for two-photon crosscorrelation and FRET analysis based solely on fluorescent proteins. Proc. Natl. Acad. Sci. USA 99, 12161-12166 (2002).
11. Heinze, K.G., Rarbach, M., Jahnz, M. & Schwille, P. Two-photon fluorescence coincidence analysis: rapid measurements of enzyme kinetics. Biophys. J. 83, 1671-1681 (2002).
12. Kim, S.A., Heinze, K.G., Waxham, M.N. & Schwille, P. Intracellular calmodulin availability accessed with two-photon cross-correlation. Proc. Natl. Acad. Sci. USA 101, 105-110 (2004).
13. Patterson, G.H. & Piston, D.W. Photobleaching in two-photon excitation microscopy. Biophys. J. 78, 2159-2162 (2000).
14. Chen, T.S., Zeng, S.Q., Luo, Q.M., Zhang, Z.H. & Zhou, W. High-order photobleaching of green fluorescent protein inside live cells in two-photon excitation microscopy. Biophys. Biochem. Res. Commun. 291, 1272-1275 (2002).
15. Matz, M.V., Lukyanov, K.A. & Lukyanov, S.A. Family of the green fluorescent protein: journey to the end of the rainbow. Bioessays 24, 953-959 (2002).
16. Labas, Y.A. et al. Diversity and evolution of the green fluorescent protein family. Proc. Natl. Acad. Sci. USA 99, 4256-4261 (2002).
17. Tsutsui, H., Karasawa, S., Shimizu, H., Nukina, N. & Miyawaki, A. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233-238 (2005).
18. Tsien, R.Y. The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544 (1998).
19. Campbell, R.E. et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 7877-7882 (2002).
20. Crivici, A. & Ikura, M. Molecular and structural basis of target recognition by calmodulin. Annu. Rev. Biophys. Biomol. Struct. 24, 85-116 (1995).
21. 2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. USA 101, 10554-10559 (2004).
22. Karasawa, S., Araki, T., Nagai, T., Mizuno, H. & Miyawaki, A. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381, 307-312 (2004).
23. Karasawa, S., Araki, T., Yamamoto-Hino, M. & Miyawaki, A. A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278, 34167-34171 (2003).
24. Sawano, A. & Miyawaki, A. Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis. Nucleic Acids Res. 28, E78 (2000).
25. Arscott, P.L. et al. Fas (CD95) expression is up-regulated on papillary thyroid carcinoma. J. Clin. Endocrinol. Metab. 84, 4246-4252 (1999).
26. Llopis, J., McCaffery, J.M., Miyawaki, A., Farquhar, M.G. & Tsien, R.Y. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95, 6803-6808 (1998).
27. Sato, S. et al. Aberrant tau phosphorylation by glycogen synthase kinase-3b and JNK3 induces oligomeric tau fibrils in COS-7 cells. J. Biol. Chem. 277, 2060-2065 (2002).
28. 2+ and protein kinase C signals using novel epifluorescence microscopy. Biophys. J. 82, 1076-1085 (2002).
29. Mochizuki, N. et al. Spatio-temporal images of growth-factor-induced activation of Ras and Rap1. Nature 411, 1065-1068 (2001).
30. 2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887 (1997).