Intracellular applications of fluorescence correlation spectroscopy: Prospects for neuroscience

Current Opinion in Neurobiology

Volumn 13, Issue 5, 2003, Pages 583-590

  Kim, Sally A ^a   Schwille, Petra ^b,c  

^a UNIVERSITY OF TEXAS   (United States)

^b MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^c DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMPA RECEPTOR; PROTEIN KINASE (CALCIUM,CALMODULIN) II;

CELL AGGREGATION; CORRELATION ANALYSIS; DIFFUSION; EQUILIBRIUM CONSTANT; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; FLUORESCENCE SPECTROSCOPY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REVIEW;

EID: 0242404444     PISSN: 09594388     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.conb.2003.09.002     Document Type: Review

References (60)

1. Bastiaens P.I., Pepperkok R. Observing proteins in their natural habitat: the living cell. Trends Biochem. Sci. 25:2000;631-637.
2. Verkman A.S. Solute and macromolecule diffusion in cellular aqueous compartments. Trends Biochem. Sci. 27:2002;27-33.
3. Minton A.P. The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media. J. Biol. Chem. 276:2001;10577-10580.
4. Lippincott-Schwartz J., Snapp E., Kenworthy A. Studying protein dynamics in living cells. Nat. Rev. Mol. Cell Biol. 2:2001;444-456 An excellent review article giving a detailed overview of various optical methods applied to studying protein dynamics in the intracellular environment.
5. Rigler R, Elson E (eds): Fluorescence Correlation Spectroscopy: Theory and Applications. Berlin: Springer; 2001.
6. Thompson N.L., Lieto A.M., Allen N.W. Recent advances in fluorescence correlation spectroscopy. Curr. Opin. Struct. Biol. 12:2002;634-641.
7. Hess S.T., Huang S., Heikal A.A., Webb W.W. Biological and chemical applications of fluorescence correlation spectroscopy: a review. Biochemistry. 41:2002;697-705.
8. Schwille P. Fluorescence correlation spectroscopy and its potential for intracellular applications. Cell Biochem. Biophys. 34:2001;383-408.
9. Elson E.L. Fluorescence correlation spectroscopy measures molecular transport in cells. Traffic. 2:2001;789-796.
10. Hink M.A., Bisselin T., Visser A.J. Imaging protein-protein interactions in living cells. Plant Mol. Biol. 50:2002;871-883.
11. Magde D., Elson E.L., Webb W.W. Thermodynamic fluctuations in a reacting system-measurement by fluorescence correlation spectroscopy. Phys. Rev. Lett. 29:1972;705-708.
12. Elson E., Magde D. Fluorescence correlation spectroscopy. I. Conceptual basis and theory. Biopolymers. 13:1974;1-27.
13. Magde D., Elson E.L., Webb W.W. Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers. 13:1974;29-61.
14. Ehrenberg M., Rigler R. Rotational Brownian motion and fluorescence intensity fluctuations. Chem. Phys. 4:1974;390-401.
15. Cluzel P., Surette M., Leibler S. An ultrasensitive bacterial motor revealed by monitoring signaling proteins in single cells. Science. 287:2000;1652-1655.
16. Koppel D.E., Axelrod D., Schlessinger J., Elson E.L., Webb W.W. Dynamics of fluorescence marker concentration as a probe of mobility. Biophys. J. 16:1976;1315-1329.
17. Fahey P.F., Koppel D.E., Barak L.S., Wolf D.E., Elson E.L., Webb W.W. Lateral diffusion in planar lipid bilayers. Science. 195:1977;305-306.
18. Wolf D.E., Schlessinger J., Elson E.L., Webb W.W., Blumenthal R., Henkart P. Diffusion and patching of macromolecules on planar lipid bilayer membranes. Biochemistry. 16:1977;3476-3483.
19. Elson E.L., Schlessinger J., Koppel D.E., Axelrod D., Webb W.W. Measurement of lateral transport on cell surfaces. Prog. Clin. Biol. Res. 9:1976;137-147.
20. Korlach J., Schwille P., Webb W.W., Feigenson G.W. Characterization of lipid bilayer phases by confocal microscopy and fluorescence correlation spectroscopy. Proc. Natl. Acad. Sci. U.S.A. 96:1999;8461-8466.
21. Schwille P., Korlach J., Webb W.W. Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes. Cytometry. 36:1999;176-182.
22. Wanaski S.P., Ng B.K., Glaser M. Caveolin scaffolding region and the membrane binding region of SRC form lateral membrane domains. Biochemistry. 42:2003;42-56.
23. A receptors and the binding of a fluorescently labeled muscimol derivative.
24. Berland K.M., So P.T., Gratton E. Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment. Biophys. J. 68:1995;694-701.
25. Politz J.C., Browne E.S., Wolf D.E., Pederson T. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. Proc. Natl. Acad. Sci. U.S.A. 95:1998;6043-6048.
26. Schwille P., Haupts U., Maiti S., Webb W.W. Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. Biophys. J. 77:1999;2251-2265.
27. Brock R., Vamosi G., Vereb G., Jovin T.M. Rapid characterization of green fluorescent protein fusion proteins on the molecular and cellular level by fluorescence correlation microscopy. Proc. Natl. Acad. Sci. U.S.A. 96:1999;10123-10128.
28. Wachsmuth M., Waldeck W., Langowski J. Anomalous diffusion of fluorescent probes inside living cell nuclei investigated by spatially-resolved fluorescence correlation spectroscopy. J. Mol. Biol. 298:2000;677-689.
29. Braun K., Peschke P., Pipkorn R., Lampel S., Wachsmuth M., Waldeck W., Friedrich E., Debus J. A biological transporter for the delivery of peptide nucleic acids (PNAs) to the nuclear compartment of living cells. J. Mol. Biol. 318:2002;237-243.
30. Kohler R.H., Schwille P., Webb W.W., Hanson M.R. Active protein transport through plastid tubules: velocity quantified by fluorescence correlation spectroscopy. J. Cell Sci. 113:2000;3921-3930.
31. Terada S., Kinjo M., Hirokawa N. Oligomeric tubulin in large transporting complex is transported via kinesin in squid giant axons. Cell. 103:2000;141-155.
32. Hess S.T., Webb W.W. Focal volume optics and experimental artifacts in confocal fluorescence correlation spectroscopy. Biophys. J. 83:2002;2300-2317.
33. Brock R., Hink M.A., Jovin T.M. Fluorescence correlation microscopy of cells in the presence of autofluorescence. Biophys. J. 75:1998;2547-2557.
34. Dittrich P., Malvezzi-Campeggi F., Jahnz M., Schwille P. Accessing molecular dynamics in cells by fluorescence correlation spectroscopy. Biol. Chem. 382:2001;491-494.
35. Chen Y., Muller J.D., Ruan Q., Gratton E. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Biophys. J. 82:2002;133-144.
36. Gennerich A., Schild D. Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used. Biophys. J. 79:2000;3294-3306.
37. Gennerich A., Schild D. Anisotropic diffusion in mitral cell dendrites revealed by fluorescence correlation spectroscopy. Biophys. J. 83:2002;510-522 This study uses FCS to measure diffusion in dendrites of cultured mitral cells of Xenopus laevis tadpoles. Confinement of the focal volume caused by the compartment restrictions of the dendrite is discussed in the context of a model of anisotropic diffusion.
38. Meyer T., Shen K. In and out of the postsynaptic region: signalling proteins on the move. Trends Cell Biol. 10:2000;238-244.
39. Post K., Pitschke M., Schafer O., Wille H., Appel T.R., Kirsch D., Mehlhorn I., Serban H., Prusiner S.B., Riesner D. Rapid acquisition of beta-sheet structure in the prion protein prior to multimer formation. Biol. Chem. 379:1998;1307-1317.
40. Pitschke M., Prior R., Haupt M., Riesner D. Detection of single amyloid beta-protein aggregates in the cerebrospinal fluid of Alzheimer's patients by fluorescence correlation spectroscopy. Nat. Med. 4:1998;832-834.
41. 2+ channels in live cells. Biophys. J. 81:2001;2639-2646.
42. Pick H., Preuss A.K., Mayer M., Wohland T., Hovius R., Vogel H. Monitoring expression and clustering of the ionotropic 5HT3 receptor in plasma membranes of live biological cells. Biochemistry. 42:2003;877-884.
43. Chen Y., Muller J.D., So P.T., Gratton E. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys. J. 77:1999;553-567.
44. Kask P., Palo K., Ullmann D., Gall K. Fluorescence-intensity distribution analysis and its application in biomolecular detection technology. Proc. Natl. Acad. Sci. U.S.A. 96:1999;13756-13761.
45. Bieschke J., Giese A., Schulz-Schaeffer W., Zerr I., Poser S., Eigen M., Kretzschmar H. Ultrasensitive detection of pathological prion protein aggregates by dual-color scanning for intensely fluorescent targets. Proc. Natl. Acad. Sci. U.S.A. 97:2000;5468-5473.
46. Teruel M.N., Meyer T. Translocation and reversible localization of signaling proteins: a dynamic future for signal transduction. Cell. 103:2000;181-184.
47. Bacia K., Schwille P. A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy. Methods. 29:2003;74-85.
48. Schwille P., Bieschke J., Oehlenschlager F. Kinetic investigations by fluorescence correlation spectroscopy: the analytical and diagnostic potential of diffusion studies. Biophys. Chem. 66:1997;211-228.
49. Schwille P., Meyer-Almes F.J., Rigler R. Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys. J. 72:1997;1878-1886.
50. Kettling U., Koltermann A., Schwille P., Eigen M. Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy. Proc. Natl. Acad. Sci. U.S.A. 95:1998;1416-1420.
51. Bieschke J., Schwille P. Aggregation of prion protein investigated in dual-color fluorescence cross-correlation spectroscopy. Fluorescence Microscopy and Fluorescent Probes. 2:1998;81-86.
52. Rigler R., Foldes-Papp Z., Meyer-Almes F.J., Sammet C., Volcker M., Schnetz A. Fluorescence cross-correlation: a new concept for polymerase chain reaction. J. Biotechnol. 63:1998;97-109.
53. Bacia K., Majoul I.V., Schwille P. Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis. Biophys. J. 83:2002;1184-1193 Here the authors utilize single-photon dual-color cross-correlation spectroscopy on the commercially available ConfoCor2 set up to study the divergence of endocytic pathways using fluorescently labeled cholera toxins.
54. Patel R.C., Kumar U., Lamb D.C., Eid J.S., Rocheville M., Grant M., Rani A., Hazlett T., Patel S.C., Gratton E., et al. Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells. Proc. Natl. Acad. Sci. U.S.A. 99:2002;3294-3299.
55. Kim S.A., Heinze K.G., Waxham M.N., Schwille P. Intracellular measurements of calmodulin and CaMKII interactions using multiphoton fluorescence cross-correlation spectroscopy (MPFCCS). Biophys. J. 82:2002;44a.
56. Heinze K.G., Koltermann A., Schwille P. Simultaneous two-photon excitation of distinct labels for dual-color fluorescence crosscorrelation analysis. Proc. Natl. Acad. Sci. U.S.A. 97:2000;10377-10382.
57. Kohl T., Heinze K.G., Kuhlemann R., Koltermann A., Schwille P. A protease assay for two-photon crosscorrelation and FRET analysis based solely on fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 99:2002;12161-12166 Two-photon excitation is combined with FRET and cross-correlation analysis for measuring a new protease assay formed on the basis of genetically encodable intrinsically fluorescent proteins. A fusion protein consisting of eGFP and DsRed separated by a peptide with a protease cleavage site was monitored for proteolytic degradation in real-time using this combination of techniques.
58. Winkler T., Kettling U., Koltermann A., Eigen M. Confocal fluorescence coincidence analysis: an approach to ultra high-throughput screening. Proc. Natl. Acad. Sci. U.S.A. 96:1999;1375-1378.
59. Heinze K.G., Rarbach M., Jahnz M., Schwille P. Two-photon fluorescence coincidence analysis: rapid measurements of enzyme kinetics. Biophys. J. 83:2002;1671-1681.
60. Heinze KG, Jahnz M, Schwille P: Triple fluorescence coincidence analysis (TFCA): direct analysis of ternary complex formation. Biophys J 2003, in press.