Flow regulates intercellular communication in HAEC by assembling functional Cx40 and Cx37 gap junctional channels

American Journal of Physiology - Heart and Circulatory Physiology

Volumn 290, Issue 5, 2006, Pages

  Ebong, Eno Essien ^a   Kim, Sanghee ^a   DePaola, Natacha ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Cell cell communication; Connexins; Endothelial cells; Fluid shear; Gap junctions

Indexed keywords

CONNEXIN 37; CONNEXIN 40; CONNEXIN 43;

AORTA; ARTICLE; CELLULAR DISTRIBUTION; ENDOTHELIUM CELL; FLUID FLOW; GAP JUNCTION; HEART MUSCLE CELL; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; TIME;

AORTA; BLOOD FLOW VELOCITY; CELL COMMUNICATION; CELLS, CULTURED; CONNEXINS; ENDOTHELIAL CELLS; GAP JUNCTIONS; HUMANS; PROTEIN BINDING;

EID: 33646406552     PISSN: 03636135     EISSN: 15221539     Source Type: Journal    
DOI: 10.1152/ajpheart.00204.2005     Document Type: Article

References (53)

1. Beblo DA and Veenstra RD. Monovalent cation permeation through the connexin40 gap junction channel: Cs, Rb, K, Na, Li, TEA, TMA, TBA, and effects of anions Br, Cl, F, acetate, aspartate, glutamate, and NO3. J Gen Physiol 109: 509-522, 1997.
2. Bennett MV, Barrio LC, Bargiello TA, Spray DC, Hertzberg E, and Sáez. Gap junctions: new tools JC, new answers, new questions. Neuron 6: 305-320, 1991.
3. Berman RS, Martin PE, Evans WH, and Griffith TM. Relative contributions of NO and gap junctional communication to endotheliumdependent relaxations of rabbit resistance arteries vary with vessel size. Microvasc Res 63: 115-128, 2002.
4. Berthoud VM, Minogue PJ, Laing JG, and Beyer EC. Pathways for degradation of connexins and gap junctions. Cardiovasc Res 62: 256-267, 2004.
5. 2+ signaling through gap junctions in airway cells. Am J Physiol Lung Cell Mol Physiol 279: L623-L630, 2000.
6. Braet K, Aspeslagh S, Vandamme W, Willecke K, Martin PE, Evans WH, and Leybaert L. Pharmacological sensitivity of ATP release triggered by photoliberation of inositol-1,4,5-trisphosphate and zero extracellular calcium in brain endothelial cells. J Cell Physiol 197: 205-213, 2003.
7. Bruzzone R, Haefliger JA, Gimlich RL, and Paul DL. Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins. Mol Biol Cell 4: 7-20, 1993.
8. Bruzzone R, White TW, and Goodenough DA. The cellular internet: on-line with connexins. Bioessays 18: 709-718, 1996.
9. Bruzzone R, White TW, and Paul DL. Connections with connexins: the molecular basis of direct intercellular signaling. Eur J Biochem 238: 1-27, 1996.
10. Carter TD, Chen XY, Carlile G, Kalapothakis E, Ogden D, and Evans WH. Porcine aortic endothelial gap junctions: identification and permeation by caged InsP3. J Cell Sci 109: 1765-1773, 1996.
11. Chaytor AT, Evans WH, and Griffith TM. Central role of heterocellular gap junctional communication in endothelium-dependent relaxations of rabbit arteries. J Physiol 508: 561-573, 1998.
12. Chaytor AT, Martin PE, Evans WH, Randall MD, and Griffith TM. The endothelial component of cannabinoid-induced relaxation in rabbit mesenteric artery depends on gap junctional communication. J Physiol 520: 539-550, 1999.
13. Christ GJ, Spray DC, el-Sabban M, Moore LK, and Brink PR. Gap junctions in vascular tissues. Evaluating the role of intercellular communication in the modulation of vasomotor tone. Circ Res 79: 631-646, 1996.
14. Cottrell GT, Wu Y, and Burt JM. Cx40 and Cx43 expression ratio influences heteromeric/ heterotypic gap junction channel properties. Am J Physiol Cell Physiol 282: C1469-C1482, 2002.
15. Cowan DB, Lye SJ, and Langille BL. Regulation of vascular connexin43 gene expression by mechanical loads. Circ Res 82: 786-793, 1998.
16. DePaola N, Davies PF, Pritchard WF Jr, Florez L, Harbeck N, and Polacek DC. Spatial and temporal regulation of gap junction connexin43 in vascular endothelial cells exposed to controlled disturbed flows in vitro. Proc Natl Acad Sci USA 96: 3154-3159, 1999.
17. De Wit C, Roos F, Bolz SS, Kirchhoff S, Krüger O, Willecke K, and Pohl U. Impaired conduction of vasodilation along arterioles in connexin40-deficient mice. Circ Res 86: 649-655, 2000.
18. Dora KA, Martin PE, Chaytor AT, Evans WH, Garland CJ, and Griffith TM. Role of heterocellular Gap junctional communication in endothelium-dependent smooth muscle hyperpolarization: inhibition by a connexin-mimetic peptide. Biochem Biophys Res Commun 254: 27-31, 1999.
19. Elfgang C, Eckert R, Lichtenberg-Fraté H, Butterweck A, Traub O, Klein TA, Hüsler DF, and Willecke K. Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. J Cell Biol 129: 805-817, 1995.
20. Evans WH and Biotano S. Connexin mimetic peptides: specific inhibitors of gap junctional intercellular communication. Biochem Soc Trans 294: 606-612, 2001.
21. Gabriels JE and Paul DL. Connexin43 is highly localized to sites of disturbed flow in rat aortic endothelium but connexin37 and connexin40 are more uniformly distributed. Circ Res 83: 636-643, 1998.
22. Griffith TM, Chaytor AT, and Edwards DH. The obligatory link: role of gap junctional communication in endothelium-dependent smooth muscle hyperpolarization. Pharmacol Res 49: 551-564, 2004.
23. Haefliger JA, Nicod P, and Meda P. Contribution of connexins to the function of the vascular wall. Cardiovasc Res 62: 345-356, 2004.
24. Haubrich S, Schwarz HJ, Bukauskas F, Lichtenberg-Frate H, Traub O, Weingart R, and Willecke K. Incompatibility of connexin 40 and 43 Hemichannels in gap junctions between mammalian cells is determined by intracellular domains. Mol Biol Cell 7: 1995-2006, 1996.
25. Hill CE, Rummery N, Hickey H, and Sandow SL. Heterogeneity in the distribution of vascular gap junctions and connexins: implications for function. Clin Exp Pharmacol Physiol 29: 620-625, 2002.
26. Hoffmann A, Gloe T, Pohl U, and Zahler S. Nitric oxide enhances de novo formation of endothelial gap junctions. Cardiovasc Res 60: 421-430, 2003.
27. Isakson BE, Evans WH, and Biotano S. Intercellular Ca2+ signaling in alveolar apithelial cells through gap junctaion and by extracellular ATP. Am J Physiol Lung Cell Mol Physiol 280: L221-L228, 2001.
28. Kumar NM and Gilula NB. The gap junction communication channel. Cell 84: 381-388, 1996.
29. Kwak BR and Jongsma HJ. Selective inhibition of gap junction channel activity by synthetic peptides. J Physiol 516: 679-685, 1999.
30. Kwak BR, Mulhaupt F, Veillard N, Gros DB, and Mach F. Altered pattern of vascular connexin expression in atherosclerotic plaques. Arterioscler Thromb Vasc Biol 22: 225-230, 2002.
31. Kwak BR, Pepper MS, Gros DB, and Meda P. Inhibition of endothelial wound repair by dominant negative connexin inhibitors. Mol Biol Cell 12: 831-845, 2001.
32. Larson DM, Haudenschild CC, and Beyer EC. Gap junction messenger RNA expression by vascular wall cells. Circ Res 66: 1074-1080, 1990.
33. Larson DM, Wrobleski MJ, Sagar GD, Westphale EM, and Beyer EC. Differential regulation of connexin43 and connexin37 in endothelial cells by cell density, growth, and TGF-beta1. Am J Physiol Cell Physiol 272: C405-C415, 1997.
34. Little TL, Beyer EC, and Duling BR. Connexin 43 and connexin 40 gap junctional proteins are present in arteriolar smooth muscle and endothelium in vivo. Am J Physiol Heart Circ Physiol 268: H729-H739, 1995.
35. Moreno AP. Biophysical properties of homomeric and heteromultimeric channels formed by cardiac connexins. Cardiovasc Res 62: 276-286, 2004.
36. Oviedo-Orta E, Errington RJ, and Evans WH. Gap junction intercellular communication during lymphocyte transendothelial migration. Cell Biol Int 26: 253-263, 2002.
37. Pepper MS, Montesano R, el Aoumari A, Gros D, Orci L, and Meda P. Coupling and connexin 43 expression in microvascular and large vessel endothelial cells. Am J Physiol Cell Physiol 262: C1246-C1257, 1992.
38. Pepper MS, Spray DC, Chanson M, Montesano R, Orci L, and Meda P. Junctional communication is induced in migrating capillary endothelial cells. J Cell Biol 109: 3027-3038, 1989.
39. Reed KE, Westphale EM, Larson DM, Wang HZ, Veenstra RD, and Beyer EC. Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein. J Clin Invest 91: 997-1004, 1993.
40. Ross R. Cell biology of atherosclerosis. Annu Rev Physiol 57: 791-804, 1995.
41. Rozental R, Srinivas M, and Spray DC. How to close a gap junction channel. Efficacies and potencies of uncoupling agents. Methods Mol Biol 154: 447-476, 2001.
42. Simon AM and McWhorter AR. Decreased intercellular dye-transfer and downregulation of non-ablated connexins in aortic endothelium deficient in connexin37 or connexin40. J Cell Sci 116: 2223-2236, 2003.
43. Valiunas V, Beyer EC, and Brink PR. Cardiac gap junction channels show quantitative differences in selectivity. Circ Res 91: 104-111, 2002.
44. Van Kempen MJ and Jongsma HJ. Distribution of connexin37, connexin40 and connexin43 in the aorta and coronary artery of several mammals. Histochem Cell Biol 112: 479-486, 1999.
45. Van Rijen H, van Kempen MJ, Analbers LJ, Rook MB, van Ginneken AC, Gros D, and Jongsma HJ. Gap junctions in human umbilical cord endothelial cells contain multiple connexins. Am J Physiol Cell Physiol 272: C117-C130, 1997.
46. Van Rijen HV, van Veen TA, Hermans MM, and Jongsma HJ. Human connexin40 gap junction channels are modulated by cAMP. Cardiovasc Res 45: 941-951, 2002.
47. Veenstra RD. Size and selectivity of gap junction channels formed from different connexins. J Bioenerg Biomembr 28:b 327-337, 1996.
48. Wang HZ and Veenstra RD. Monovalent ion selectivity sequences of the rat connexin43 gap junction channel. J Gen Physiol 109: 491-507, 1997.
49. White TW and Bruzzone R. Multiple connexin proteins in single intercellular channels: connexin compatibility and functional consequences. J Bioenerg Biomembr 28: 339-350, 1996.
50. Wong CW, Christen T, and Kwak BR. Connexins in leukocytes: shuttling messages? Cardiovasc Res 62: 357-367, 2004.
51. Xie HQ and Hu VW. Modulation of gap junctions in senescent endothelial cells. Exp Cell Res 214: 172-176, 1994.
52. Yeh HI, Chang HM, Lu WW, Lee YN, Ko YS, Severs NJ, and Tsai CH. Age-related alteration of gap junction distribution and connexin expression in rat aortic endothelium. J Histochem Cytochem 48: 1377-1389, 2000.
53. Yeh HI, Rothery S, Dupont E, Coppen SR, and Severs NJ. Individual gap junction plaques contain multiple connexins in arterial endothelium. Circ Res 83: 1248-1263, 1998.