Differential regulation of connexin43 and connexin37 in endothelial cells by cell density, growth, and TFG-β1

American Journal of Physiology - Cell Physiology

Volumn 272, Issue 2 41-2, 1997, Pages

  Larson, D M ^a   Wrobleski, M J ^a   Sagar, G D V ^a   Westphale, E M ^b   Beyer, E C ^b  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

aorta; bovine; gap junctions; immunoblot; immunocytochemistry; intercellular communication; RNA; vascular

Indexed keywords

CONNEXIN 37; CONNEXIN 43; GAP JUNCTION PROTEIN; TRANSFORMING GROWTH FACTOR BETA1; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL COMMUNICATION; CELL DENSITY; CELL GROWTH; CELL JUNCTION; CONTROLLED STUDY; ENDOTHELIUM CELL; IMMUNOCYTOCHEMISTRY; MESSENGER RNA SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS REGULATION; REGULATORY MECHANISM; RNA ANALYSIS;

ANIMALIA; BOVINAE;

EID: 0030901646     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.1997.272.2.c405     Document Type: Article

References (35)

1. Beyer, E. C. Gap junctions. Int. Rev. Cytol. 137C: 1-37, 1993.
2. Beyer, E. C., J. Kistler, D. L. Paul, and D. A. Goodenough. Antisera directed against Connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues. J. Cell Biol. 108: 595-605, 1989.
3. Beyer, E. C., D. L. Paul, and D. A. Goodenough. Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J. Cell Biol. 105: 2621-2629, 1987.
4. Beyer, E. C., K. E. Reed, E. M. Westphale, H. L. Kanter, and D. M. Larson. Molecular cloning and expression of rat connexin40, a gap junction protein expressed in vascular smooth muscle. J. Membr. Biol. 127: 69-76, 1992.
5. Border, W. A., and E. Ruoslahti. Transforming growth factor-β in disease: the dark side of tissue repair. J. Clin. Invest. 90: 1-7, 1992.
6. Brand, T., and M. D. Schneider. The TGF-β superfamily in myocardium: ligands, receptors, transduction, and function. J. Mol. Cell. Cardiol. 27: 5-18, 1995.
7. Chandross, K. J., M. Chanson, D. C. Spray, and J. A. Kessler. Transforming growth factor-beta 1 and forskolin modulate gap junctional communication and cellular phenotype of cultured Schwann cells. J. Neurosci. 15: 262-273, 1995.
8. Chiba, H., N. Sawada, M. Oyamada, T. Kojima, K. Iba, S. Ishii, and M. Mori. Hormonal regulation of connexin43 expression and gap junctional communication in human osteoblastic cells. Cell Struct. Funct. 19: 173-177, 1994.
9. Christ, G. J. Modulation of alpha 1-adrenergic contractility in isolated vascular tissues by heptanol: a functional demonstration of the potential importance of intercellular communication to vascular response generation. Life Sci. 56: 709-721, 1995.
10. Gibson, D. F., M. Z. Hossain, G. S. Goldberg, P. Acevedo, and J. S. Bertram. The mitogenic effects of transforming growth factors beta 1 and beta 2 in C3H/10T1/2 cells occur in the presence of enhanced gap junctional communication. Cell Growth & Differ. 5: 687-696, 1994.
11. Gordon, R. E., B. P. Lane, and M. Marin. Regeneration of rat tracheal epithelium: changes in gap junctions during specific phases of the cell cycle. Exp. Lung Res. 3: 47-56, 1982.
12. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature Lond. 227: 680-685, 1970.
13. Laing, J. G., and E. C. Beyer. The gap junction protein Connexin43 is degraded via the ubiquitin proteosomal pathway. J. Biol. Chem. 270: 26399-26403, 1995.
14. Laird, D. W., M. Castillo, and L. Kasprzak. Gap junction turnover, intracellular trafficking and phosphorylation of Connexin43 in Brefeldin A-treated rat mammary tumor cells. J. Cell Biol. 131: 1193-1203, 1995.
15. Larson, D. M. Intercellular junctions and junctional transfer in the blood vessel wall. In: Endothelial Cells, edited by U. S. Ryan. Boca Raton, FL: CRC, 1988, vol. 3, p. 75-88.
16. Larson, D. M. Junctional communication and the wound healing response. In: Cell Intercommunication, edited by W. C. DeMello. Boca Raton, FL: CRC, 1990, p. 93-109.
17. Larson, D. M., K. Beatrice, C. C. Haudenschild, E. M. Westphale, and E. C. Beyer. Modulation of expression of two gap junction proteins by TGF-β1 in vascular endothelial cells (Abstract). Mol. Biol. Cell 3: 292a, 1992.
18. Larson, D. M., and C. C. Haudenschild. Junctional transfer in wounded cultures of bovine aortic endothelial cells. Lab. Invest. 59: 373-379, 1988.
19. Larson, D. M., C. C. Haudenschild, and E. C. Beyer. Gap junction messenger RNA expression by vascular wall cells. Circ. Res. 66: 1074-1080, 1990.
20. Larson, D. M., M. J. Wrobleski, S. Agarwal, G. D. V. Sagar, B. Lall, E. M. Westphale, and E. C. Beyer. Differential expression of Connexin37 and Connexin43 in vascular endothelial cells is modulated by growth (Abstract). Mol. Biol. Cell 6: 298a, 1995.
21. Little, T. L., E. C. Beyer, and B. R. Duling. Connexin43 and connexin 40 gap junction proteins are present in arteriolar smooth muscle and endothelium in vivo. Am. J. Physiol. 268 (Heart Circ. Physiol. 37): H729-H739, 1995.
22. Loewenstein, W. R. Junctional intercellular communication and the control of growth. Biochim. Biophys. Acta 560: 1-65, 1979.
23. Maldonado, P. E., B. Rose, and W. R. Loewenstein. Growth factors modulate junctional cell-to-cell communication. J. Membr. Biol. 106: 203-210, 1989.
24. Mehta, P. P., A. Hotz-Wagenblatt, B. Rose, D. Shalloway, and W. R. Loewenstein. Incorporation of the gene for a cell-cell channel protein into transformed cells leads to normalization of growth. J. Membr. Biol. 124: 207-225, 1991.
25. Musil, L. S., E. C. Beyer, and D. A. Goodenough. Expression of the gap junction protein connexin43 in embryonic chick lens: molecular cloning, ultrastructural localization, and posttranslational phosphorylation. J. Membr. Biol. 116: 163-175, 1990.
26. Nikol, S., J. M. Isner, J. G. Pickering, M. Kearney, G. Leclerc, and L. Weir. Expression of transforming growth factor-β1 is increased in human vascular restenosis lesions. J. Clin. Invest. 90: 1582-1592, 1992.
27. Pepper, M. S., and P. Meda. Basic fibroblast growth factor increases junctional communication and connexin43 expression in microvascular endothelial cells. J. Cell. Physiol. 153: 196-205, 1992.
28. Pepper, M. S., R. Montesano, A. El Aoumari, D. Gros, L. Orci and P. Meda. Coupling and connexin43 expression in microvascular and large vessel endothelial cells. Am. J. Physiol. 262 (Cell Physiol. 31): C1246-C1257, 1992.
29. Reaume, A. G., P. A. de Sousa, S. Kulkarni, B. L. Langille, D. Zhu, T. C. Davies, S. C. Juneja, G. M. Kidder, and J. Rossant. Cardiac malformation in neonatal mice lacking connexin43. Science Wash. DC 267: 1831-1834, 1995.
30. Reed, K. E., E. M. Westphale, D. M. Larson, H.-Z. Wang, R. D. Veenstra, and E. C. Beyer. Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein. J. Clin. Invest. 91: 997-1004, 1993.
31. Segal, S. S., and B. R. Duling. Propagation of vasodilation in resistance vessels of the hamster: development and review of a working hypothesis. Circ. Res. 61, Suppl. II: II-20-II-25, 1987.
32. Sutton, A. B., A. E. Canfield, S. L. Schor, M. E. Grant, and A. M. Schor. The response of endothelial cells to TGF β-1 is dependent on cell shape, proliferative state and the nature of the substratum. J. Cell Sci. 99: 777-787, 1991.
33. Thompson, N. L., F. Bazoberry, E. H. Spier, W. Casscells, V. J. Ferrans, K. C. Flanders, P. Kondaiah, A. G. Geiser, and M. B. Sporn. Transforming growth factor beta-1 in acute myocardial infarction in rats. Growth Factors 1: 91-99, 1988.
34. Van Zoelen, E. J. J., and L. G. J. Tertoolen. Transforming growth factor-β enhances the extent of intercellular communication between normal rat kidney cells. J. Biol. Chem. 266: 12075-12081, 1991.
35. Veenstra, R. D., H.-Z. Wang, D. A. Beblo, M. G. Chilton, A. L. Harris, E. C. Beyer, and P. R. Brink. Selectivity of connexin-specific gap junctions does not correlate with channel conductance. Circ. Res. 77: 1156-1165, 1995.