Connexin43 is highly localized to sites of disturbed flow in rat aortic endothelium but connexin37 and connexin40 are more uniformly distributed

Circulation Research

Volumn 83, Issue 6, 1998, Pages 636-643

  Gabriels, Joseph E ^a   Paul, David L ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Aorta; Circulation; Connexin; Endothelium; Gap junction

Indexed keywords

CONNEXIN 43; GAP JUNCTION PROTEIN;

ABDOMINAL AORTA; ANIMAL EXPERIMENT; ARTICLE; BLOOD FLOW; ENDOTHELIUM CELL; FEMALE; GAP JUNCTION; IMMUNOFLUORESCENCE MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; SHEAR STRESS; THORACIC AORTA; VASCULAR ENDOTHELIUM;

EID: 0032556026     PISSN: 00097330     EISSN: None     Source Type: Journal    
DOI: 10.1161/01.RES.83.6.636     Document Type: Article

References (49)

1. Larson DM, Haudenschild CC. Junctional transfer in wounded cultures of bovine aortic endothelial cells. Lab Invest. 1988;59:373-379.
2. Larson DM, Haudenschild CC, Beyer EC. Gap junction messenger RNA expression by vascular wall cells. Circ Res. 1990;66:1074-1080.
3. Segal SS, Duling BR. Flow control among microvessels coordinated by intercellular conduction. Science. 1986;234:868-870.
4. Segal SS, Duling BR. Conduction of vasomotor responses in arterioles: a role for cell-to-cell coupling? Am J Physiol. 1989;256:H838-H345.
5. Davies PF, Olesen SP, Clapham DE, Morrel EM, Schoen FJ. Endothelial communication: state of the art lecture. Hypertension. 1988;11:563-572.
6. Beny JL, Pacicca C. Bidirectional electrical communication between smooth muscle and endothelial cells in the pig coronary artery. Am J Physiol. 1994;266:H1465-H1472.
7. Pepper MS, Spray DC, Chanson M, Montesano R, Orci L, Meda P. Junctional communication is induced in migrating capillary endothelial cells. J Cell Biol. 1989;109:3027-3038.
8. White TW, Bruzzone R, Paul DL. The connexin family of intercellular channel forming proteins [review]. Kidney Int. 1995;48:1148-1157.
9. Yeh HI, Dupont E, Coppen S, Rothery S, Severs NJ. Gap junction localization and connexin expression in cytochemically identified endothelial cells of arterial tissue. J Histochem Cytochem. 1997;45:539-550.
10. Bruzzone R, Haefliger J-A, Gimlich RL, Paul DL. Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins. Mol Biol Cell. 1993;4:7-20.
11. Hennemann H, Suchyna T, Lichtenberg-Fraté H. Jungbluth L, Dahl E, Schwarz J, Nicholson BJ, Willecke K. Molecular cloning and functional expression of mouse connexin40, a second gap junction gene preferentially expressed in lung. J Cell Biol. 1992;117:1299-1310.
12. Haefliger J-A, Bruzzone R, Jenkins NA, Gilbert DJ, Copeland NG, Paul DL. Four novel members of the connexin family of gap junction proteins: molecular cloning, expression, and chromosome mapping. J Biol Chem. 1992;267:2057-2064.
13. Reed KE, Westphale EM, Larson DM, Wang Z, Veenstra RD, Beyer EC. Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein. J Clin Invest. 1993;91:997-1004.
14. Vanrijen HVM, Vankempen MJA, Analbers LJS, Rook MB, Vanginneken ACG, Gros D, Jongsma HJ. Gap junctions in human umbilical cord endothelial cells contain multiple connexins. Am J Physiol. 1997; 41:C117-C130.
15. Delorme B, Dahl E, Jarry-Guichard T, Briand J-P, Willecke K, Gros D, Théveniau-Ruissy M. Expression pattern of connexin gene products at the early developmental stages of the mouse cardiovascular system. Circ Res. 1997;81:423-437.
16. Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988;67:31-40.
17. Beyer EC, Paul DL, Goodenough DA. Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J Cell Biol. 1987;105:2621-2629.
18. Beyers TJ, Armstrong PB. Membrane protein redistribution during Xenopus first cleavage. J Cell Biol. 1986;102:2176-2184.
19. Goliger JA, Paul DL. Expression of gap junction proteins Cx26, Cx31.1, Cx37, and Cx43 in developing and mature rat epidermis. Dev Dyn. 1994;200:1-13.
20. Haubrich S, Schwarz HJ, Bukauskas FF, Lichtenberg-Fraté H, Traub O, Weingart R, Willecke K. Incompatibility of connexin 40 and 43 hemichannels in gap junctions between mammalian cells is determined by intracellular domains. Mol Biol Cell. 1996;7:1995-2006.
21. Anayiotos AS, Jones SA, Giddens DP, Glagov S, Zarins CK. Shear stress at a compliant model of the human carotid bifurcation. J Biomech Eng. 1994;116:98-106.
22. Barbee KA, Davies PF, Lal R. Shear stress-induced reorganization of the surface topography of living endothelial cells imaged by atomic force microscopy. Circ Res. 1994;74:163-171.
23. Bassiouny HS, Zarins CK, Kadowaki MH, Glagov S. Hemodynamic stress and experimental aortoiliac atherosclerosis. J Vasc Surg. 1994;19: 426-434.
24. Bussolari SR, Dewey CF Jr, Gimbrone MA Jr. Apparatus for subjecting living cells to fluid shear stress. Rev Sci Instrum. 1982;53:1851-1854.
25. Cornhill JF, Roach MR. A quantitative study of the localization of atherosclerotic lesions in the rabbit aorta. Atherosclerosis. 1976;23: 489-501.
26. Davies PF, Robotewskyj A, Griem ML, Dull RO, Polacek DC. Hemodynamic forces and vascular cell communication in arteries. Arch Pathol Lab Med. 1992;116:1301-1306.
27. DePaola N, Gimbrone MAJ, Davies PF, Dewey CFJ. Vascular endothelium responds to fluid shear stress gradients [published correction appears in Arterioscler Thromb. 1993;13:465], Arterioscler Thromb. 1992;12: 1254-1257.
28. DePaola N, Gimbrone MAJ, Davies PF, Dewey CFJ. Vascular endothelium responds to fluid shear stress gradients [published correction appears in Arterioscler Thromb. 1993;13:465], Arterioscler Thromb. 1992;12: 1254-1257.
29. Glagov S, Zarins C, Giddens DP, Ku DN. Hemodynamics and atherosclerosis: insights and perspectives gained from studies of human arteries. Arch Pathol Lab Med. 1988;112:1018-1031.
30. Resnick N, Gimbrone MAJ. Hemodynamic forces are complex regulators of endothelial gene expression. FASEB J. 1995;9:874-882.
31. Nagel T, Resnick N, Atkinson WJ, Dewey CFJ, Gimbrone MAJ. Shear stress selectively upregulates intercellular adhesion molecule-1 expression in cultured human vascular endothelial cells. J Clin Invest. 1994;94:885-891.
32. Langille BL, Reidy MA, Kline RL. Injury and repair of endothelium at sites of flow disturbances near abdominal aortic coarctations in rabbits. Arteriosclerosis. 1986;6:146-154.
33. Yu W, Dahl G, Werner R. The connexin43 gene is responsive to oestrogen. Proc R Soc Lond B Biol Sci. 1994;255:2-132.
34. Sullivan R, Ruangvoravat CP, Joo D, Morgan J, Wang BL, Wang XK, Lo CW. Structure, sequence and expression of the mouse Cx43 gene encoding connexin 43. Gene. 1993;130:191-199.
35. Lau AF, Kurata WE, Kanemitsu MY, Loo LW, Warn-Cramer BJ, Eckhart W, Lampe PD. Regulation of connexin43 function by activated tyrosine protein kinases. J Bioenerg Biomembr. 1996;28:359-368.
36. Pepper MS, Meda P. Basic fibroblast growth factor increases junctional communication and connexin43 expression in microvascular endothelial cells. J Cell Physiol. 1992;153:196-205.
37. Davies PF, Remuzzi A, Gordon EJ, Dewey CFJ, Gimbrone MAJ. Turbulent fluid shear stress induces vascular endothelial cell turnover in vitro. Proc Natl Acad Sci USA. 1986;83:2114-2117.
38. Yu QC, McNeil PL. Transient disruptions of aortic endothelial cell plasma membranes. Am J Pathol. 1992;141:1349-1360.
39. Beny JL, Gribi F. Dye and electrical coupling of endothelial cells in situ. Tissue Cell. 1989;21:797-802.
40. Little TL, Beyer EC, Duling BR. Connexin 43 and connexin 40 gap junctional proteins are present in arteriolar smooth muscle and endothelium in vivo. Am J Physiol. 1995;268:H729-H739.
41. Pepper MS, Montesano R, El Aoumari A, Gros D, Orci L, Meda P. Coupling and connexin43 expression in microvascular and large vessel endothelial cells. Am J Physiol. 1992;262:C1246-C1257.
42. White TW. Paul DL, Goodenough DA, Bruzzone R. Functional analysis of selective interactions among rodent connexins. Mol Biol Cell. 1995;6: 459-470.
43. Beny JL. Endothelial and smooth muscle cells hyperpolarized by bradykinin are not dye coupled. Am J Physiol. 1990;258:H836-H841.
44. Segal SS, Beny JL. Intracellular recording and dye transfer in arterioles during blood flow control. Am J Physiol. 1992;263:H1-H7.
45. Polacek D, Lal R. Volin MV, Davies PF. Gap junctional communication between vascular cells: induction of connexin43 messenger RNA in macrophage foam cells of atherosclerotic lesions. Am J Pathol. 1993;142: 593-606.
46. Beyer EC, Steinberg TH. Evidence that the gap junction protein connexin-43 is the ATP-induced pore of mouse macrophages. J Biol Chem. 1991;266:7971-7974.
47. Navab M, Liao F, Hough GP, Ross LA, Van Lenten BJ, Rajavashisth TB, Lusis AJ, Laks H, Drinkwater DC, Fogelman AM. Interaction of monocytes with cocultures of human aortic wall cells involves interleukins 1 and 6 with marked increases in connexin43 message. J Clin Invest. 1991;87:1763-1772.
48. Jara PI, Boric MP, Saez JC. Leukocytes express connexin 43 after activation with lipopolysaccharide and appear to form gap junctions with endothelial cells after ischemia- reperfusion. Proc Natl Acad Sci U S A. 1995;92:7011-7015.
49. Krenacs T, Rosendaal M. Immunohistological detection of gap junctions in human lymphoid tissue: connexin43 in follicular dendritic and lymphoendothelial cells. J Histochem Cytochem. 1995;43:1125-1137.