Molecular analyses of KCNQ1-5 potassium channel mRNAs in rat and guinea pig inner ears: Expression, cloning, and alternative splicing

Acta Oto-Laryngologica

Volumn 126, Issue 4, 2006, Pages 346-352

  Liang, Gui Hua ^a,b,c   Jin, Zhe ^a,b   Ulfendahl, Mats ^a,b   Järlebark, Leif ^a,b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^c GUANGXI MEDICAL UNIVERSITY   (China)

Author keywords

Alternative splicing; Cochlea; Gene expression; Guinea pig; Molecular sequence data; Rat; Reverse transcriptase polymerase chain reaction; Voltage gated potassium channels

Indexed keywords

DNA; MESSENGER RNA; POTASSIUM CHANNEL KCNQ1; RNA;

ALTERNATIVE RNA SPLICING; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; EXON; GENE; GENE DISRUPTION; GENE EXPRESSION; GENE SEQUENCE; GUINEA PIG; HEARING IMPAIRMENT; MOLECULAR CLONING; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPECTROPHOTOMETRY;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; COCHLEA; DNA PRIMERS; GENE EXPRESSION; GUINEA PIGS; KCNQ POTASSIUM CHANNELS; MOLECULAR SEQUENCE DATA; POTASSIUM CHANNELS, VOLTAGE-GATED; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 33646371284     PISSN: 00016489     EISSN: None     Source Type: Journal    
DOI: 10.1080/00016480500416777     Document Type: Article

References (35)

1. + cycling and the endocochlear potential. Hear Res 2002;165:1-9.
2. Steel KP, Kros CJ. A genetic approach to understanding auditory function. Nat Genet 2001;27:143-9.
3. Patuzzi R. The Goldman-Hodgkin-Katz equation and graphical'load-line' analysis of ionic flow through outer hair cells. Hear Res 1998;125:71-97.
4. Liang G, Moore EJ, Ulfendahl M, Rydqvist B, Järlebark L. An M-like potassium current in the guinea pig cochlea. ORL 2005; 67:75-82.
5. Kubisch C, Schroeder BC, Friedrich T, Lutjohann B, El-Amraoui A, Marlin S, et al. KCNQ4, a novel potassium channel expressed in sensory outer hair cells, is mutated in dominant deafness. Cell 1999;96:437-46.
6. + current in a vertebrate neurone. Nature 1980;283:673-6.
7. Zhang H, Craciun LC, Mirshahi T, Rohács T, Lopes CM, Jin T, et al. PIP2 activates KCNQ channels, and its hydrolysis underlies receptor-mediated inhibition of M currents. Neuron 2003;37:963-75.
8. Lerche C, Scherer CR, Seebohm G, Derst C, Wei AD, Busch AE, et al. Molecular cloning and functional expression of KCNQ5, a potassium channel subunit that may contribute to neuronal M-current diversity. J Biol Chem 2000;275:22395-400.
9. Schroeder BC, Hechenberger M, Weinreich F, Kubisch C, Jentsch TJ. KCNQ5, a novel potassium channel broadly expressed in brain, mediates M-type currents. J Biol Chem 2000;275:24089-95.
10. Marcotti W, Kros CJ. Developmental expression of the potassium current IK,n contributes to maturation of mouse outer hair cells. J Physiol 1999;520:653-60.
11. Jentsch TJ. Neuronal KCNQ potassium channels: physiology and role in disease. Nat Rev Neurosci 2000;1:21-30.
12. Robbins J. KCNQ potassium channels: physiology, pathophysiology, and pharmacology. Pharmacol Ther 2001;90:1-19.
13. Biervert C, Schroeder BC, Kubisch C, Berkovic SF, Propping P, Jentsch TJ, et al. A potassium channel mutation in neonatal human epilepsy. Science 1998;279:403-6.
14. Charlier C, Singh NA, Ryan SG, Lewis TB, Reus BE, Leach RJ, et al. A pore mutation in a novel KQT-like potassium channel gene in an idiopathic epilepsy family. Nat Genet 1998;18:53-5.
15. Singh NA, Charlier C, Stauffer D, DuPont BR, Leach RJ, Melis R, et al. A novel potassium channel gene, KCNQ2, is mutated in an inherited epilepsy of newborns. Nat Genet 1998;18:25-9.
16. Beisel KW, Nelson NC, Delimont DC, Fritzsch B. Longitudinal gradients of KCNQ4 expression in spiral ganglion and cochlear hair cells correlate with progressive hearing loss in DFNA2. Mol Brain Res 2000;82:137-49.
17. + channel mutated in a form of dominant deafness, is expressed in the inner ear and the central auditory pathway. Proc Natl Acad Sci U S A 2000;97:4333-8.
18. + channels in brain. J Neurosci 2001;21:4609-24.
19. Cooper EC, Harrington E, Jan YN, Jan LY. M channel KCNQ2 subunits are localized to key sites for control of neuronal network oscillations and synchronization in mouse brain. J Neurosci 2001;21:9529-40.
20. Nakamura M, Watanabe H, Kubo Y, Yokoyama M, Matsumoto T, Sasai H, et al. KQT2, a new putative potassium channel family produced by alternative splicing. Isolation, genomic structure, and alternative splicing of the putative potassium channels. Receptors Channels 1998;5:255-71.
21. Tinel N, Lauritzen I, Chouabe C, Lazdunski M, Borsotto M. The KCNQ2 potassium channel: splice variants, functional and developmental expression. Brain localization and comparison with KCNQ3. FEBS Lett 1998;438:171-6.
22. Biervert C, Steinlein OK. Structural and mutational analysis of KCNQ2, the major gene locus for benign familial neonatal convulsions. Hum Genet 1999;104:234-40.
23. Pan Z, Selyanko AA, Hadley JK, Brown DA, Dixon JE, McKinnon D. Alternative splicing of KCNQ2 potassium channel transcripts contributes to the functional diversity of M-currents. J Physiol 2001;531:347-58.
24. Smith JS, Iannotti CA, Dargis P, Christian EP, Aiyar J. Differential expression of kcnq2 splice variants: implications to m current function during neuronal development. J Neurosci 2001;21:1096-103.
25. Schmitt N, Schwarz M, Peretz A, Abitbol I, Attali B, Pongs O. A recessive C-terminal Jervell and Lange-Nielsen mutation of the KCNQ1 channel impairs subunit assembly. EMBO J 2000;19:332-40.
26. Adams PR, Brown DA, Constanti A. Pharmacological inhibition of the M-current. J Physiol 1982;332:223-62.
27. Wang HS, McKinnon D. Potassium currents in rat prevertebral and paravertebral sympathetic neurones: control of firing properties. J Physiol 1995;485:319-35.
28. Constanti A, Galvan M. M-current in voltage-clamped olfactory cortex neurones. Neurosci Lett 1983;39:65-70.
29. + channel that accelerates the rod response to dim light. Neuron 1989;3:573-81.
30. Bialer M, Johannessen SI, Kupferberg HJ, Levy RH, Perucca E, Tomson T. Progress report on new antiepileptic drugs: a summary of the Seventh Eilat Conference (EILAT VII). Epilepsy Res 2004;61:1-48.
31. Wulfsen I, Hauber HP, Schiemann D, Bauer CK, Schwartz JR. Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary. J Neuroendocrinol 2000;12:263-72.
32. Jow F, Wang K. Cloning and functional expression of rKCNQ2 K(+) channel from rat brain. Mol Brain Res 2000;80:269-78.
33. Kananura C, Biervert C, Hechenberger M, Engels H, Steinlein OK. The new voltage gated potassium channel KCNQ5 and neonatal convulsions. Neuroreport 2000;11:2063-7.
34. Kreisberg MS, Silldorff EP, Pallone TL. Localization of adenosine-receptor subtype mRNA in rat outer medullary descending vasa recta by RT-PCR. Am J Physiol 1997;272:H1231-8.
35. Beisel KW, Rocha-Sanchez SM, Morris KA, Nie L, Feng F, Kachar B, et al. Differential expression of KCNQ4 in inner hair cells and sensory neurons is the basis of progressive high-frequency hearing loss. J Neurosci 2005;25:9285-93.