Inverse flux analysis for reduction of acetate excretion in Escherichia coli

Biotechnology Progress

Volumn 13, Issue 4, 1997, Pages 361-367

  Delgado, Javier ^a   Liao, James C ^a  

^a Texas AandM University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY; CATALYSIS; CELL CULTURE; COMPOSITION EFFECTS; COMPUTATIONAL METHODS; ENZYMES; ESTERS; GROWTH KINETICS; METABOLISM; STOICHIOMETRY;

ACETATE EXCRETION; ANAPLEROTIC PATHWAYS; ESCHERICHIA COLI; INVERSE FLUX ANALYSIS;

COLIFORM BACTERIA;

ACETIC ACID DERIVATIVE;

ARTICLE; ESCHERICHIA COLI; METABOLISM;

ACETATES; ESCHERICHIA COLI;

ESCHERICHIA COLI;

EID: 0031194875     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp970047x     Document Type: Article

References (21)

1. Bauer, K. A. A.; Ben-Bassat, A.; Dawson, M.; de la Puente, V. T.; Neway, J. O.; Improved expression of human interleukin-2 in high-cell-density fermentation cultures of Escherichia coli K-12 by a phosphotransacetylase mutant. Appl. Environ. Microbiol. 1990, 56, 1296-1302.
2. Chao, Y. P.; Liao, J. C. Alteration of growth yield by overexpression of ppc and pck in Escherichia coli. Appl. Environ. Microbiol. 1993, 59, 4261-4265.
3. Chou, C.-H.; Bennett, G. N.; San, K.-Y. Effect of modified glucose uptake using genetic engineering techniques on high-level recombinant protein production in Escherichia coli dense cultures. Biotechnol. Bioeng. 1994, 44, 952-960.
4. Diaz-Ricci, J. C.; Regan, L.; Bailey, J. E. Effect of alteration of the acetic acid synthesis pathway on the fermentation pattern of Escherichia coli. Biotechnol. Bioeng. 1991, 38, 1318-1324.
5. Farmer, W. R.; Liao, J. C. Reduction of Aerobic Acetate Production by Escherichia coli. Appl. Env. Microbiol. 1997, in press.
6. Goel, A.; Ferrance, J.; Jeong, J.; Ataai, M. Analysis of metabolic fluxes in batch and continuous cultures of Bacillus subtilis. Biotechnol. Bioeng. 1993, 42, 686-696.
7. Goel, A.; Lee, J.; Domach, M. M.; Ataai, M. M. Suppressed acid formation by co-feeding of glucose and citrate in Bacillus cultures: Emergence of pyruvate kinase as a potential metabolic engineering site. Biotechnol. Prog. 1995, 11, 380-385.
8. Heinrich, R.; Rapoport, T. A. A linear steady-state treatment of enzymatic chains: General properties, control and effector strength. Eur. J. Biochem. 1974, 42, 89-95.
9. Holms, W. H. The central metabolic pathways in Escherichia coli: relationship between flux and control at a branchpoint, efficiency of conversion to biomass, and excretion of acetate. Curr. Top. Cell. Regul. 1986, 28, 69-105.
10. Jorgensen, H.; Nielsen, J.; Villadsen, J.; Mollgaard, H. Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations. Biotechnol. Bioeng. 1995, 46, 117-131.
11. Kacser, H.; Burns, J. A. The control of flux. In Rate control of biological processes; Davies, D. D., Ed.; Cambridge University Press: Cambridge, U.K., 1973; p 65.
12. Kleman, G. L.; Strohl, W. R. Acetate metabolism by Escherichia coli in high-cell-density fermentation. Appl. Environ. Microbiol. 1994, 60, 3952-3958.
13. Ko, Y. F.; Bentley, W. E.; Weigand, W. A. An integrated metabolic modeling approach to describe the energy efficiency of Escherichia coli under oxygen-limited conditions: cellular energetics and carbon flux. Biotechnol. Bioeng. 1993, 42, 843-853.
14. Liao, J. C.; Delgado, J. Advances in metabolic control analysis. Biotechnol. Prog. 1993, 9, 221-233.
15. Majewski, R. A.; Domach, M. M. Simple constrained-optimization view of acetate overflow in Escherichia coli. Biotechnol. Bioeng. 1990, 35, 732-738.
16. Neidhardt, F. C.; Ingraham, J. L.; Schaechter, M. Physiology of the Bacterial Cell. A Molecular Approach; Sinauer Associates, Inc.: Sunderland, MA, 1990.
17. Nimmo, H. G. The tricarboxylic acid cycle and anerplerotic reactions. In Escherichia coli and Salmonella typhimurium; Neidhardt, F. C., Ingraham, J. L., Low, K. B., Magasanik, B., Schaechter, M., Umbarger, H. E., Eds.; ASM: Washington, DC, 1987; p 156.
18. Shiloach, J.; Kaufman, J.; Guillard, A. S.; Fass, R. Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (IDE3) and Escherichia coli JM109. Biotechnol. Bioeng. 1996, 49, 421-428.
19. Stephanopoulos, G.; Vallino, J. J. Network regidity and metabolic engineering in metabolite overproduction. Science 1991, 252, 1675-1681.
20. Vallino, J. J.; Stephanopoulos, G. N. Flux Determination in Cellular Bioreaction Networks: Applications to Lysine Fermentations. In Frontiers in Bioprocessing; Sikdar, S. K., Bier, M., Todd, P., Eds.; CRC Press, Inc.: Boca Raton, FL, 1989; pp 205-219.
21. Varma, A.; Boesch, B. W.; Palsson, B. O. Stoichiometric interpretation of Escherichia coli glucose catabolism under various oxygen rates. Appl. Environ. Microbiol. 1993, 59, 2465-2473.