Oxygen-glucose deprivation decreases glutathione levels and glutamate uptake in rat hippocampal slices

Brain Research

Volumn 1083, Issue 1, 2006, Pages 211-218

  Brongholi, Karina ^a   Souza, Daniela G ^a   Bainy, Afonso C D ^a   Dafre, Alcir L ^a   Tasca, Carla I ^a  

^a FEDERAL UNIVERSITY OF SANTA CATARINA   (Brazil)

Author keywords

Glutamate uptake; Glutathione; Hippocampal slice; Oxidative stress; Oxygen glucose deprivation

Indexed keywords

ANTIOXIDANT; CATALASE; DITHIOTHREITOL; GLUCOSE; GLUTAMATE TRANSPORTER; GLUTAMIC ACID; GLUTATHIONE; GLUTATHIONE REDUCTASE; OXYGEN; PEROXIDASE; PROTEIN; SODIUM; SUPEROXIDE DISMUTASE; THIOL;

AMINO ACID TRANSPORT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN CELL; CELL DAMAGE; CELL DEATH; CELL LEVEL; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ACTIVITY; HIPPOCAMPUS; ISCHEMIA; MALE; NEUROTOXICITY; NONHUMAN; OXIDATIVE STRESS; OXYGEN SUPPLY; PRIORITY JOURNAL; PROTEIN CONTENT; RAT; REPERFUSION; SYNAPSE;

AMINO ACID TRANSPORT SYSTEM X-AG; ANIMALS; ANTIOXIDANTS; CATALASE; CELL SURVIVAL; DITHIOTHREITOL; DOWN-REGULATION; GLUTAMIC ACID; GLUTATHIONE; GLUTATHIONE REDUCTASE; HIPPOCAMPUS; HYPOXIA-ISCHEMIA, BRAIN; MALE; ORGAN CULTURE TECHNIQUES; OXIDATIVE STRESS; PEROXIDASE; RATS; RATS, WISTAR; REPERFUSION INJURY; SUPEROXIDE DISMUTASE; SYNAPTIC MEMBRANES; SYNAPTIC TRANSMISSION;

EID: 33645980998     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.brainres.2006.02.003     Document Type: Article

References (45)

1. Aebi H. Catalase in vitro. Methods Enzymol. 105 (1984) 121-126
2. Akerboom T.P., and Sies H. Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol. 77 (1981) 373-382
3. Ames B.N., Shigenaga M.K., and Hagen T.M. Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. U. S. A. 90 (1993) 7915-7922
4. Aoyama K., Suh S.W., Hamby A.M., Liu J., Chan W.Y., Chen Y., and Swanson R.A. Neuronal glutathione deficiency and age-dependent neurodegeneration in the EAAC1 deficient mouse. Nat. Neurosci. 9 (2006) 119-126
5. Arias R.L., Tasse J.R., and Bowlby M.R. Neuroprotective interaction effects of NMDA and AMPA receptor antagonists in an in vitro model of cerebral ischemia. Brain Res. 816 (1999) 299-308
6. Bannai S., and Kitamura E. Transport interaction of l-cystine and l-glutamate in human diploid fibroblasts in culture. J. Biol. Chem. 255 (1980) 2372-2376
7. Benveniste H., Drejer J., Schousboe A., and Diemer N.H. Elevation of the extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischemia monitored by intracerebral microdialysis. J. Neurochem. 43 (1984) 1369-1374
8. Chen Y., and Swanson R.A. The glutamate transporters EAAT2 and EAAT3 mediate cysteine uptake in cortical neuron cultures. J. Neurochem. 84 (2003) 1332-1339
9. Chen Y., Ying W., Simma V., Copin J.C., Chan P.H., and Swanson R.A. Overexpression of Cu,Zn superoxide dismutase attenuates oxidative inhibition of astrocyte glutamate uptake. J. Neurochem. 75 (2000) 939-945
10. Choi D.W. Glutamate neurotoxicity and diseases of the nervous system. Neuron 1 (1988) 623-634
11. Cooper A.J., and Kristal B.S. Multiple roles of glutathione in the central nervous system. Biol. Chem. 378 (1997) 793-802
12. Coyle J.T., and Puttfarcken P. Oxidative stress, glutamate, and neurodegenerative disorders. Science 262 (1993) 689-695
13. Crapo J.D., McCord J.M., and Fridovich I. Preparation and assay of superoxide dismutases. Methods Enzymol. 53 (1978) 382-393
14. Dafre A.L., Arteni N.S., Siqueira I.R., and Netto C.A. Perturbations in the thiol homeostasis following neonatal cerebral hypoxia-ischemia in rats. Neurosci. Lett. 345 (2003) 65-68
15. Dickinson D.A., Levonen A.L., Moellering D.R., Arnold E.K., Zhang H., Darley-Usmar V.M., and Forman H.J. Human glutamate cysteine ligase gene regulation through the electrophile response element. Free Radical Biol. Med. 37 (2004) 1152-1159
16. Dringen R. Metabolism and functions of glutathione in brain. Prog. Neurobiol. 62 (2000) 649-671
17. Ellman G.L. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82 (1959) 70-77
18. Garthwaite G., Williams G.D., and Garthwaite J. Glutamate toxicity: an experimental and theoretical analysis. Eur. J. Neurosci. 4 (1992) 353-360
19. Giustarini D., Rossi R., Milzani A., Colombo R., and Dalle-Donne I. S-glutathionylation: from redox regulation of protein functions to human diseases. J. Cell. Mol. Med. 8 (2004) 201-212
20. Jenner P. Oxidative damage in neurodegenerative disease. Lancet 344 (1994) 796-798
21. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
22. Matsuda T., Shimizu I., Murata Y., and Baba A. Glucose and oxygen deprivation induces a Ca(2+)-mediated decrease in (Na(+)+K+)-ATPase activity in rat brain slices. Brain Res. 576 (1992) 263-270
23. Mawatari K., Yasui Y., Sugitani K., Takadera T., and Kato S. Reactive oxygen species involved in the glutamate toxicity of C6 glioma cells via xc antiporter system. Neuroscience 73 (1996) 201-208
24. Meister A. Glutathione metabolism and function via the gamma-glutamyl cycle. Life Sci. 15 (1974) 177-190
25. Molz S., Decker H., I.Oliveira J.L., Souza D.O., and Tasca C.I. Neurotoxicity induced by glutamate in glucose-deprived rat hippocampal slices is prevented by GMP. Neurochem. Res. 30 (2005) 83-89
26. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65 (1983) 55-63
27. Murphy T.H., Miyamoto M., Sastre A., Schnaar R.L., and Coyle J.T. Glutamate toxicity in a neuronal cell line involves inhibition of cystine transport leading to oxidative stress. Neuron 2 (1989) 1547-1558
28. Pocock J.M., and Nicholls D.G. Exocytotic and nonexocytotic modes of glutamate release from cultured cerebellar granule cells during chemical ischaemia. J. Neurochem. 70 (1998) 806-813
29. Nishizawa Y. Glutamate release and neuronal damage in ischemia. Life Sci. 69 (2001) 369-381
30. Oka A., Belliveau M.J., Rosenberg P.A., and Volpe J.J. Vulnerability of oligodendroglia to glutamate: pharmacology, mechanisms, and prevention. J. Neurosci. 13 (1993) 1441-1453
31. Oliveira I.J.L., Molz S., Souza D.O., and Tasca C.I. Neuroprotective effect of GMP in hippocampal slices exposed to an in vitro model of ischemia. Cell. Mol. Neurobiol. 22 (2002) 335-344
32. O'Regan M.H., Song D., VanderHeide S.J., and Phillis J.W. Free radicals and the ischemia-evoked extracellular accumulation of amino acids in rat cerebral cortex. Neurochem. Res. 22 (1997) 273-280
33. Phillis J.W., and O'Regan M.H. Characterization of modes of release of amino acids in the ischemic/reperfused rat cerebral cortex. Neurochem. Int. 43 (2003) 461-467
34. Pong K. Oxidative stress in neurodegenerative diseases: therapeutic implications for superoxide dismutase mimetics. Expert Opin. Biol. Ther. 3 (2003) 127-139
35. +]: involvement of transporter reversal and mitochondrial calcium. J. Neurochem. 80 (2002) 706-714
36. Rossi D.J., Oshima T., and Attwell D. Glutamate release in severe brain ischaemia is mainly by reversed uptake. Nature 403 (2000) 316-321
37. Schmidt-Kastner R., and Freund T.F. Selective vulnerability of the hippocampus in brain ischemia. Neuroscience 40 (1991) 599-636
38. Sies H., Koch O.R., Martino E., and Boveris A. Increased biliary glutathione disulfide release in chronically ethanol-treated rats. FEBS Lett. 103 (1979) 287-290
39. Strasser U., and Fischer G. Quantitative measurement of neuronal degeneration in organotypic hippocampal cultures after combined oxygen/glucose deprivation. J. Neurosci. Methods 57 (1995) 177-186
40. Tan S., Wood M., and Maher P. Oxidative stress induces a form of programmed cell death with characteristics of both apoptosis and necrosis in neuronal cells. J. Neurochem. 71 (1998) 95-105
41. Trotti D., Danbolt N.C., and Volterra A. Glutamate transporters are oxidant-vulnerable: a molecular link between oxidative and excitotoxic neurodegeneration?. Trends Pharmacol. Sci. 19 (1998) 328-334
42. Turrens J.F. Mitochondrial formation of reactive oxygen species. J. Physiol. 552 (2003) 335-344
43. Wang X.F., and Cynader M.S. Astrocytes provide cysteine to neurons by releasing glutathione. J. Neurochem. 74 (2000) 1434-1442
44. Warner D.S., Sheng H., and Batinic-Haberle I. Oxidants, antioxidants and the ischemic brain. J. Exp. Biol. 207 (2004) 3221-3231
45. Whitaker J.F. Lactate dehydrogenase as a cytoplasmatic marker in brain. Clin. Chim. Acta 24 (1969) 23-27