Validation of DRG-like F11 cells for evaluation of KCNQ/M-channel modulators

Assay and Drug Development Technologies

Volumn 4, Issue 1, 2006, Pages 49-56

  Jow, Flora ^a   He, Lan ^a   Kramer, Angela ^a   Hinson, Joe ^a   Bowlby, Mark R ^a   Dunlop, John ^a   Wang, KeWei ^a  

^a PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

10,10 BIS(4 PYRIDINYLMETHYL) 9(10H) ANTHRACENONE; NEUROPEPTIDE RECEPTOR; NEUROTRANSMITTER; POTASSIUM CHANNEL; POTASSIUM CHANNEL BLOCKING AGENT; POTASSIUM CHANNEL KCNQ2; POTASSIUM CHANNEL KCNQ3; RUBIDIUM ION; UNCLASSIFIED DRUG; VOLTAGE GATED CALCIUM CHANNEL;

ANIMAL CELL; ARTICLE; ATOMIC ABSORPTION; CELL CULTURE; CONTROLLED STUDY; FUNCTIONAL ASSESSMENT; IN VITRO STUDY; MEMBRANE ELECTROPHYSIOLOGY; MOLECULAR MODEL; NERVE CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PATCH CLAMP; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SPINAL GANGLION; VALIDATION PROCESS; WHOLE CELL;

ANIMALS; CYANATES; DNA PRIMERS; ELAPID VENOMS; GANGLIA, SPINAL; KCNQ2 POTASSIUM CHANNEL; KINETICS; NEURONS, AFFERENT; PATCH-CLAMP TECHNIQUES; RATS; RUBIDIUM; TETRAETHYLAMMONIUM;

EID: 33645319457     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2006.4.49     Document Type: Article

References (20)

1. + currents. J Neurosci Res 1995;42:493-503.
2. Platika D, Boulos MH, Baizer L, Fishman MC: Neuronal traits of clonal cell lines derived by fusion of dorsal root ganglia neurons with neuroblastoma cells. Proc Natl Acad Sci U S A 1985;82:3499-3503.
3. - conductance in a mouse neuroblastoma × rat dorsal root ganglion cell line (F11). Brain Res 1993;614:178-184.
4. + currents in F11 neurons and neuroblastoma × DRG neuron hybrid cells via cholera toxin-sensitive receptors. Brain Res 1993;605:214-220.
5. + conductance of DRG neurons and neuroblastoma × neuron hybrid F11 cells. Brain Res 1995;696:97-105.
6. Wang HS, Pan Z, Shi W, Brown BS, Wymore RS, Coher IS, Dixon JE, McKinnon D: KCNQ2 and KCNQ3 potassium channel subunits: molecular correlates of the M-channel. Science 198;282:1890-1893.
7. Biervert C, Schroeder BC, Kubisch C, Berkovic SF, Propping P. Jentsch TJ, Steinlein OK: A potassium channel mutation in neonatal human epilepsy. Science 1998;279: 403-406.
8. Castaldo P, del Giudice EM, Coppola G, Pascotto A, Annunziato L, Taglialatela M: Benign familial neonatal convulsions caused by altered gating of KCNQ2/KCNQ3 potassium channels. J Neurosci 2002;22:RC199.
9. Charlier C, Singh NA, Ryan SG, Lewis TB, Reus BE, Leach RJ, Leppert M: A pore mutation in a novel KQT-like potassium channel gene in an idiopathic epilepsy family. Nat Genet 1998;18:53-55.
10. Watanabe H, Nagata E, Kosakai A, Nakamura M, Yokoyama M, Tanaka K, Sasai H: Disruption of the epilepsy KCNQ2 gene results in neural hyperexcitability. J Neurochem 2000;75:28-33.
11. Passmore GM, Selyanko AA, Mistry M, Al-Qatari M, Marsh SJ, Matthews EA, Dickenson AH, Brown TA, Burbidge SA, Main M, Brown DA: KCNQ/M currents in sensory neurons: significance for pain therapy. J Neurosci 2003;23:7227-7236.
12. Dost R, Rostock A, Rundfeldt C: The anti-hyperalgesic activity of retigabine is mediated by KCNQ potassium channel activation. Naunyn Schmiedebergs Arch Pharmacol 2004;369:382-390.
13. Schroder RL, Jespersen T, Christophersen P. Strobaek D, Jensen BS, Olesen SP: KCNQ4 channel activation by BMS-204352 and retigabine. Neuropharmacology 2001; 40:888-898.
14. Blackburn-Munro G, Jensen BS: The anticonvulsant retigabine attenuates nociceptive behaviours in rat models of persistent and neuropathic pain. Eur J Pharmacol 2003; 460:109-116.
15. Gribkoff VK: The therapeutic potential of neuronal KCNQ channel modulators. Expert Opin Ther Targets 2003;7: 737-748.
16. Rundfeldt C, Netzer R: The novel anticonvulsant retigabine activates M-currents in Chinese hamster ovary-cells transfected with human KCNQ2/3 subunits. Neurosci Lett 200;282:73-77.
17. Tatulian L, Brown DA: Effect of the KCNQ potassium channel opener retigabine on single KCNQ2/3 channels expressed in CHO cells. J Physiol 2003;549:57-63.
18. + current in a vertebrate neurone. Nature 1980;283:673-676.
19. Tatulian L, Delmas P, Abogadie FC, Brown DA: Activation of expressed KCNQ potassium currents and native neuronal M-type potassium currents by the anti-convulsant drug retigabine. J Neurosci 2001;21:5535-5545.
20. Wang K, McIlvain B, Tseng E, Kowal D, Jow F, Shen R, Zhang H, Shan QJ, He L, Chen D, Lu Q, Dunlop J: Validation of an atomic absorption rubidium ion efflux assay for KCNQ/M-channels using the Ion Channel Reader 8000. Assay Drug Dev Technol 2004;2:525-534.