Germ line transcription in mice bearing neor gene downstream of Iγ3 exon in the Ig heavy chain locus

International Immunology

Volumn 18, Issue 4, 2006, Pages 581-589

  Samara, Maha ^a,b   Oruc, Zeliha ^a   Dougier, Hei Lanne ^a   Essawi, Tamer ^b   Cogné, Michel ^a   Khamlichi, Ahmed Amine ^a,c  

^a CNRS   (France)

^b BIRZEIT UNIVERSITY   (Palestine)

^c INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

Author keywords

B lymphocyte; Class switch recombination; Long range interaction; Splicing

Indexed keywords

GENE PRODUCT; IMMUNOGLOBULIN HEAVY CHAIN; NEOMYCIN; NEOMYCIN RESISTANCE PROTEIN; PROTEIN NEO R; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHIMERA; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXON; FLOW CYTOMETRY; GENE INSERTION; GENE INTERACTION; GENETIC CODE; GENETIC TRANSCRIPTION; GERM LINE; IN VITRO STUDY; INTRON; MOUSE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; SPLEEN CELL;

ALTERNATIVE SPLICING; ANIMALS; B-LYMPHOCYTES; BASE SEQUENCE; BLOTTING, NORTHERN; DRUG RESISTANCE; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GENES, IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN CLASS SWITCHING; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; MICE; MICE, MUTANT STRAINS; MOLECULAR SEQUENCE DATA; NEOMYCIN; PROMOTER REGIONS (GENETICS); REGULATORY SEQUENCES, NUCLEIC ACID; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSCRIPTION, GENETIC;

EID: 33645243055     PISSN: 09538178     EISSN: 14602377     Source Type: Journal    
DOI: 10.1093/intimm/dxh400     Document Type: Article

References (40)

1. Stavnezer, J. 2000. Molecular processes that regulate class switching. Curr. Top. Microbiol. Immunol. 245:127.
2. Radbruch, A., Muller, W. and Rajewsky, K. 1986. Class switch recombination is IgG1 specific on active and inactive IgH loci of IgG1-secreting B-cell blasts. Proc. Natl. Acad. Sci. USA 83:954.
3. Hummel, M., Berry, J. K. and Dunnick, W. 1987. Switch region content of hybridomas: The two spleen cell Igh loci tend to rearrange to the same isotype. J. Immunol. 138:3539.
4. Winter, E., Krawinkel, U. and Radbruch, A. 1987. Directed Ig class switch recombination in activated murine B cells. EMBO J. 6:1663.
5. Delpy, L., Le Bert, M., Cogné, M. and Khamlichi, A. A. 2003. Germ-line transcription occurs on both the functional and the non-functional alleles of immunoglobulin constant heavy chain genes. Eur. J. Immunol. 33:2108.
6. Chaudhury, J. and Alt, F. W. 2004. Class-switch recombination: Interplay of transcription, DNA deamination and DNA repair. Nat. Rev. Immunol. 4:541.
7. Reaban, M. E. and Griffin, J. A. 1990. Induction of RNA-stabilized DNA conformers by transcription of an immunoglobulin switch region. Nature 348:342.
8. Reaban, M. E., Lebowitz, J. and Griffin, J. A. 1994. Transcription induces the formation of a stable RNA.DNA hybrid in the immunoglobulin alpha switch region. J. Biol. Chem. 269:21850.
9. Daniels, G. A. and Lieber, M. R. 1995. RNA:DNA complex formation upon transcription of immunoglobulin switch regions: Implications for the mechanism and regulation of class switch recombination. Nucleic Acids Res. 23:5006.
10. Mizuta, R., Iwai, K., Shigeno, M. et al. 2003. Molecular visualization of immunoglobulin switch region RNA/DNA complex by atomic force microscope. J. Biol. Chem. 278:4431.
11. Tian, M. and Alt, F. W. 2000. Transcription-induced cleavage of immunoglobulin switch regions by nucleotide excision repair nucleases in vitro. J. Biol. Chem. 275:24163.
12. Yu, K., Chedin, F., Hsieh, C. L., Wilson, T. E. and Lieber, M. R. 2003. R-loops at immunoglobulin class switch regions in the chromosomes of stimulated B cells. Nat. Immunol. 4:442.
13. Nambu, Y., Sugai, M., Gonda, H. et al. 2003. Transcription-coupled events associating with immunoglobulin switch region chromatin. Science 302:2137.
14. Zhang, J., Bottaro, A., Li, S. C., Stewart, V. and Alt, F. W. 1993. A selective defect in IgG2b switching as a result of targeted mutation of the I gamma 2b promoter and exon. EMBO J. 12:3529.
15. Jung, S., Rajewsky, K. and Radbruch, A. 1993. Shutdown of class switch recombination by deletion of a switch region control element. Science 259:984.
16. Bottaro, A., Lansford, R., Xu, L., Zhang, J., Rothman, P. and Alt, F. W. 1994. S region transcription per se promotes basal IgE class switch recombination but additional factors regulate the efficiency of the process. EMBO J. 13:665.
17. Harriman, G. R., Bradley, A., Das, S., Rogers-Fani, P. and Davis, A. C. 1996. IgA class switch in I alpha exon-deficient mice. Role of germline transcription in class switch recombination. J. Clin. Invest. 97:477.
18. r cassette provides required functions of the Iγ2b exon for class switching. Int. Immunol. 10:1683.
19. Lorenz, M., Jung, S. and Radbruch, A. 1995. Switch transcripts in immunoglobulin class switching. Science 267:1825.
20. Hein, K., Lorenz, M. G. O., Siebenkotten, G., Petry, K., Christine, R. and Radbruch, A. 1998. Processing of switch transcripts is required for targeting of antibody class switch recombination. J. Exp. Med. 188:2369.
21. Qiu, G., Harriman, G. R. and Stavnezer, J. 1999. 1α exon-replacement mice synthesize a spliced HPRT-Cα transcript which may explain their ability to switch to IgA. Inhibition of switching to IgG in these mice. Int. Immunol. 11:37.
22. Kuzin, I. I., Ugine, G. D., Wu, D., Young, F., Chen, J. and Bottaro, A. 2000. Normal isotype switching in B cells lacking the Iμ exon splice donor site: Evidence for multiple Iμ-like germline transcripts. J. Immunol. 164:1451.
23. Cogné, M., Lansford, R., Bottaro, A. et al. 1994. A class switch control region at the 3′ end of the immunoglobulin heavy chain locus. Cell 77:737.
24. Manis, J. P., Vander Stoep, N., Tian, M. et al. 1998. Class switching in B cells lacking 3′ immunoglobulin heavy chain enhancers. J. Exp. Med. 188:1421.
25. Khamlichi, A. A., Pinaud, E., Decourt, C., Chauveau, C. and Cogné, M. 2000. The 3′ IgH regulatory region: A complex structure in a search for a function. Adv. Immunol. 75:317.
26. Pinaud, E., Khamlichi, A. A., Le Morvan, C. et al. 2001. Localization of the 3′ IgH locus elements that effect long-distance regulation of class switch recombination. Immunity 15:187.
27. Manis J. P., Tian, M. and Alt, F. W. 2002. Mechanism and control of class-switch recombination. Trends Immunol. 23:31.
28. Harriman, G. R., Bogue, M., Rogers, P. et al. 1999. Targeted deletion of the IgA constant region in mice leads to IgA deficiency with alterations in expression of other Ig isotypes. J. Immunol. 162:2521.
29. r cassettes inserted into the immunoglobulin heavy chain constant region locus. Proc. Natl. Acad. Sci. USA 96:3000.
30. Khamlichi, A. A., Glaudet, F., Oruc, Z., Denis, V., Le Bert, M. and Cogné, M. 2004. Immunoglobulin class switch recombination in mice devoid of any Sp tandem repeat. Blood 103:3828.
31. Li, S. C., Rothman, P. B., Zhang, J., Chan, C., Hirsh, D. and Alt, F. W. 1994. Expression of Iμ-Cγ hybrid germline transcripts subsequent to immunoglobulin heavy chain class switching. Int. Immunol. 6:491.
32. Delpy, L., Decourt, C., Le Bert, M. and Cogné, M. 2002. B cell development arrest upon insertion of a neo gene between JH and Eμ: promoter competition results in transcriptional silencing of germline JH and complete V(D)J rearrangements. J. Immunol. 169:6875.
33. Cockerill, P. N. 1990. Nuclear matrix attachment occurs in several regions of the IgH locus. Nucleic Acids Res. 18:2643.
34. Manis, J. P., Michaelson, J. S., Birshtein, B. K. and Alt, F. W. 2003. Elucidation of a downstream boundary of the 3′ IgH regulatory region. Mol. Immunol. 39:753.
35. Bulger, M. and Groudine, M. 1999. Looping versus linking: Toward a model for long-distance gene activation. Genes Dev. 13:2465.
36. Blackwood, E. M. and Kadonaga, J. T. 1998. Going the distance: A current view of enhancer action. Science 281:61.
37. Xu, M. Z. and Stavnezer, J. 1992. Regulation of transcription of immunoglobulin germ-line gamma 1 RNA: Analysis of the promoter/enhancer. EMBO J. 11:145.
38. Elenich, L. A., Ford, C. S. and Dunnick, W. A. 1996. The gamma 1 heavy chain gene includes all of the cis-acting elements necessary for expression of properly regulated germ-line transcripts. J. Immunol. 157:176.
39. Cunningham, K., Ackerly, H., Alt, F. W. and Dunnick, W. A. 1998. Germline transcription and recombination of a murine VDJmudel-tagamma1 transgene. Int. Immunol. 10:527.
40. Adams, K., Ackerly, H., Cunningham, K. and Dunnick, W. 2000. A DNase I hypersensitive site near the murine gamma1 switch region contributes to insertion site independence of transgenes and modulates the amount of transcripts induced by CD40 ligation. Int. Immunol. 12:1705.