Quantitative 3D fluorescence technique for the analysis of en face preparations of arterial walls using quantum dot nanocrystals and two-photon excitation laser scanning microscopy

American Journal of Physiology - Regulatory Integrative and Comparative Physiology

Volumn 290, Issue 1, 2006, Pages

  Ferrara, Dardo E ^a   Weiss, Daiana ^a   Carnell, Peter H ^b   Vito, Ray P ^b   Vega, David ^a   Gao, Xiaohu ^c   Nie, Shuming ^c   Taylor, W Robert ^a,d  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^c EMORY UNIVERSITY   (United States)

^d VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

Atherosclerosis; Multiphoton microscopy; Semiconductor nanocrystals; Shear stress; Vascular cell adhesion molecule 1

Indexed keywords

ENDOTHELIAL NITRIC OXIDE SYNTHASE; NANOCRYSTAL; QUANTUM DOT; VASCULAR CELL ADHESION MOLECULE 1; VON WILLEBRAND FACTOR; APOLIPOPROTEIN E;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; AORTA; ARTERY WALL; ARTICLE; AUTOFLUORESCENCE; CELLULAR DISTRIBUTION; CORONARY ARTERY; CORONARY HEMODYNAMICS; EXCITATION; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; LASER MICROSCOPY; MOUSE; MULTIPHOTON MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SHEAR STRESS; THREE DIMENSIONAL IMAGING; ANIMAL; C57BL MOUSE; CONFOCAL MICROSCOPY; CORONARY ARTERY ATHEROSCLEROSIS; CORONARY BLOOD VESSEL; CYTOLOGY; ENDOTHELIUM CELL; FLUORESCENCE; GENE EXPRESSION REGULATION; GENETICS; MALE; METABOLISM; METHODOLOGY; MOUSE MUTANT;

ANIMALS; AORTA; APOLIPOPROTEINS E; CORONARY ARTERIOSCLEROSIS; CORONARY VESSELS; ENDOTHELIAL CELLS; FLUORESCENCE; GENE EXPRESSION REGULATION; HUMANS; MALE; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; MICROSCOPY, CONFOCAL; QUANTUM DOTS; VASCULAR CELL ADHESION MOLECULE-1;

EID: 33644845852     PISSN: 03636119     EISSN: 15221490     Source Type: Journal    
DOI: 10.1152/ajpregu.00449.2005     Document Type: Article

References (41)

1. Azuma K, Watada H, Niihashi M, Otsuka A, Sato F, Kawasumi M, Shimada S, Tanaka Y, Kawamori R, and Mitsumata M. A new en face method is useful to quantitate endothelial damage in vivo. Biochem Biophys Res Commun 309: 384-390, 2003.
2. Baschong W, Suetterlin R, and Laeng RH. Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM). J Histochem Cytochem 49: 1565-1572, 2001.
3. Chan WC, Maxwell DJ, Gao X, Bailey RE, Han M, and Nie S. Luminescent quantum dots for multiplexed biological detection and imaging. Curr Opin Biotechnol 13: 40-46, 2002.
4. Chan WCW and Nie SM. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 282: 2016-2018, 1998.
5. Chen J, Tung CH, Mahmood U, Ntziachristos V, Gyurko R, Fishman MC, Huang PL, and Weissleder R. In vivo imaging of proteolytic activity in atherosclerosis. Circulation 105: 2766-2771, 2002.
6. Dai G, Kaazempur-Mofrad MR, Natarajan S, Zhang Y, Vaughn S, Blackman BR, Kamm RD, Garcia-Cardena G, and Gimbrone MA Jr. Distinct endothelial phenotypes evoked by arterial waveforms derived from atherosclerosis-susceptible and -resistant regions of human vasculature. Proc Natl Acad Sci USA 101: 14871-14876, 2004.
7. Denk W, Strickler JH, and Webb WW. Two-photon laser scanning fluorescence microscopy. Science 248: 73-76, 1990.
8. Dobrucki JW. Confocal microscopy: quantitative analytical capabilities. Methods Cell Biol 75: 41-72, 2004.
9. Donners MM, Verluyten MJ, Bouwman FG, Mariman EC, Devreese B, Vanrobaeys F, van Beeumen J, van den Akker LH, Daemen MJ, and Heeneman S. Proteomic analysis of differential protein expression in human atherosclerotic plaque progression. J Pathol 206: 39-45, 2005.
10. Gao X, Cui Y, Levenson RM, Chung LW, and Nie S. In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol 22: 969-976, 2004.
11. Gao X and Nie S. Molecular profiling of single cells and tissue specimens with quantum dots. Trends Biotechnol 21: 371-373, 2003.
12. Hajra L, Evans AI, Chen M, Hyduk SJ, Collins T, and Cybulsky MI. The NF-kappa B signal transduction pathway in aortic endothelial cells is primed for activation in regions predisposed to atherosclerotic lesion formation. Proc Natl Acad Sci USA 97: 9052-9057, 2000.
13. Henninger DD, Panes J, Eppihimer M, Russell J, Gerritsen M, Anderson DC, and Granger DN. Cytokine-induced VCAM-1 and ICAM-1 expression in different organs of the mouse. J Immunol 158: 1825-1832, 1997.
14. Iiyama K, Hajra L, Iiyama M, Li H, DiChiara M, Medoff BD, and Cybulsky MI. Patterns of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 expression in rabbit and mouse atherosclerotic lesions and at sites predisposed to lesion formation. Circ Res 85: 199-207, 1999.
15. Jaiswal JK and Simon SM. Potentials and pitfalls of fluorescent quantum dots for biological imaging. Trends Cell Biol 14: 497-504, 2004.
16. Kelly KA, Allport JR, Tsourkas A, Shinde-Patil VR, Josephson L, and Weissleder R. Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle. Circ Res 96: 327-336, 2005.
17. Khan BV, Parthasarathy SS, Alexander RW, and Medford RM. Modified low-density lipoprotein and its constituents augment cytokine-activated vascular cell adhesion molecule-1 gene expression in human vascular endothelial cells. J Clin Invest 95: 1262-1270, 1995.
18. Kim S, Lim YT, Soltesz EG, De Grand AM, Lee J, Nakayama A, Parker JA, Mihaljevic T, Laurence RG, Dor DM, Cohn LH, Bawendi MG, and Frangioni JV. Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping. Nat Biotechnol 22: 93-97, 2004.
19. Kingsley K, Carroll K, Huff JL, and Plopper GE. Photobleaching of arterial autofluorescence for immunofluorescence applications. Biotechniques 30: 794-797, 2001.
20. Klinkner AM, Bugelski PJ, Waites CR, Louden C, Hart TK, and Kerns WD. A novel technique for mapping the lipid composition of atherosclerotic fatty streaks by en face fluorescence microscopy. J Histochem Cytochem 45: 743-753, 1997.
21. Konig K. Multiphoton microscopy in life sciences. J Microsc 200: 83-104, 2000.
22. Lacoste TD, Michalet X, Pinaud F, Chemla DS, Alivisatos AP, and Weiss S. Ultrahigh-resolution multicolor colocalization of single fluorescent probes. Proc Natl Acad Sci USA 97: 9461-9466, 2000.
23. Larson DR, Zipfel WR, Williams RM, Clark SW, Bruchez MP, Wise FW, and Webb WW. Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science 300: 1434-1436, 2003.
24. Libby P. Inflammation in atherosclerosis. Nature 420: 868-874, 2002.
25. McGillicuddy CJ, Carrier MJ, and Weinberg PD. Distribution of lipid deposits around aortic branches of mice lacking LDL receptors and apolipoprotein E. Arterioscler Thromb Vasc Biol 21: 1220 -1225, 2001.
26. Michalet X, Kapanidis AN, Laurence T, Pinaud F, Doose S, Pflughoefft M, and Weiss S. The power and prospects of fluorescence microscopies and spectroscopies. Annu Rev Biophys Biomol Struct 32: 161-182, 2003.
27. Michalet X, Lacoste TD, and Weiss S. Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes. Methods 25: 87-102, 2001.
28. Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, and Weiss S. Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307: 538-544, 2005.
29. Millonig G, Niederegger H, and Wick G. Analysis of the cellular composition of the arterial intima with modified en face techniques. Lab Invest 81: 639-641, 2001.
30. Nakashima Y, Raines EW, Plump AS, Breslow JL, and Ross R. Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the ApoE-deficient mouse. Arterioscler Thromb Vasc Biol 18: 842-851, 1998.
31. Nerem RM, Alexander RW, Chappell DC, Medford RM, Varner SE, and Taylor WR. The study of the influence of flow on vascular endothelial biology. Am J Med Sci 316: 169-175, 1998.
32. Ogiwara N, Usuda N, Yamada M, Johkura K, Kametani K, and Nakazawa A. Quantification of protein A-gold staining for peroxisomal enzymes by confocal laser scanning microscopy. J Histochem Cytochem 47: 1343-1349, 1999.
33. Pawley J. The 39 steps: a cautionary tale of quantitative 3-D fluorescence microscopy. Biotechniques 28: 884-886, 888, 2000.
34. Rubart M. Two-photon microscopy of cells and tissue. Circ Res 95: 1154-1166, 2004.
35. Sorescu D, Weiss D, Lassegue B, Clempus RE, Szocs K, Sorescu GP, Valppu L, Quinn MT, Lambeth JD, Vega JD, Taylor WR, and Griendling KK. Superoxide production and expression of nox family proteins in human atherosclerosis. Circulation 105: 1429-1435, 2002.
36. Stroh M, Zimmer JP, Duda DG, Levchenko TS, Cohen KS, Brown ED, Scadden DT, Torchilin VP, Bawendi MG, Fukumura D, and Jain RK. Quantum dots spectrally distinguish multiple species within the tumor milieu in vivo. Nat Med 11: 678-682, 2005.
37. Tangirala R, Rubin E, and Palinski W. Quantitation of atherosclerosis in murine models: correlation between lesions in the aortic origin and in the entire aorta, and differences in the extent of lesions between sexes in LDL receptor-deficient and apolipoprotein E-deficient mice. J Lipid Res 36: 2320-2328, 1995.
38. Tokumasu F and Dvorak J. Development and application of quantum dots for immunocytochemistry of human erythrocytes. J Microsc 211: 256-261, 2003.
39. van Zandvoort M, Engels W, Douma K, Beckers L, Oude Egbrink M, Daemen M, and Slaaf DW. Two-photon microscopy for imaging of the (atherosclerotic) vascular wall: a proof of concept study. J Vasc Res 41: 54-63, 2004.
40. Voura EB, Jaiswal JK, Mattoussi H, and Simon SM. Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy. Nat Med 10: 993-998, 2004.
41. Zipfel WR, Williams RM, and Webb WW. Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol 21: 1369-1377, 2003.