Ultrastructural investigations on protective effects of NCX 4016 (nitroaspirin) on macrovascular endothelium in diabetic Wistar rats

Journal of Submicroscopic Cytology and Pathology

Volumn 37, Issue 2, 2005, Pages 205-213

  Ambrosini, Maria Vittoria ^a   Mariucci, G ^a   Rambotti, M G ^a   Tantucci, M ^b   Covarelli, C ^b   De Angelis, L ^b   Del Soldato, P ^c  

^a UNIVERSITY OF PERUGIA   (Italy)

^b Perugia General Hospital   (Italy)

^c NicOx SA   (France)

Author keywords

Aortic endothelium; Diabetes; NCX 4016; Nitric oxide donating aspirin; Rat; Scanning electron microscopy; Transmission electron microscopy

Indexed keywords

RATTUS NORVEGICUS;

ACETYLSALICYLIC ACID; ACETYLSALICYLIC ACID 3 (NITROXYMETHYL)PHENYL ESTER; DRUG DERIVATIVE; NONSTEROID ANTIINFLAMMATORY AGENT;

ANIMAL; AORTA; ARTICLE; DRUG EFFECT; EXPERIMENTAL DIABETES MELLITUS; MALE; PATHOLOGY; RAT; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE; VASCULAR DISEASE; VASCULAR ENDOTHELIUM; WISTAR RAT;

ANIMALS; ANTI-INFLAMMATORY AGENTS, NON-STEROIDAL; AORTA; ASPIRIN; DIABETES MELLITUS, EXPERIMENTAL; ENDOTHELIUM, VASCULAR; MALE; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; RATS; RATS, WISTAR; VASCULAR DISEASES;

EID: 33644791351     PISSN: 11229497     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. AHLUWALIA A., FOSTER P., SCOTLAND R.S., MCLEAN P.G., MATHUR A., PERRETTI M., MONCADA S. and HOBBS A.J., 2004. Antiinflammatory activity of soluble guanylate cyclase: cGMP-dependent down-regulation of P-selectin expression and leukocyte recruitment. Proc. Natl. Acad. Sci. USA, 101, 1386-1391.
2. CLARK J.M. and GLAGOV S., 1976. Luminal surface of distended arteries by scanning electron microscopy: eliminating configurational and technical artefacts. Br. J. Exp. Pathol., 57, 129-135.
3. DAVIDGE S.T., 2001. Prostaglandin H synthase and vascular function. Circ. Res., 89, 650-660.
4. DAVIES P.F. and BOWYER D.E., 1975. Scanning electron microscopy: arterial endothelial integrity after fixation at physiological pressure. Atherosclerosis, 21, 463-469.
5. DEL SOLDATO P., SORRENTINO R. and PINTO A., 1999. NO-aspirins: a class of new anti-inflammatory and antithrombotic agents. Trends Pharmacol. Sci., 20, 319-323.
6. FIORUCCI S., 2001. NO-releasing NSAIDs are caspase inhibitors. Trends Immunol., 22, 232-235.
7. FIORUCCI S., ANTONELLI E., SANTUCCI L., MORELLI O., MIGLIETTI M., FEDERICI B., MANNUCCI R., DEL SOLDATO P. and MORELLI A., 1999. Gastrintestinal safety of nitric oxide-derived aspirin is related to inhibition of ICE-like cysteine protease. Gastroenterology, 116, 1089-1106.
8. FIORUCCI S., MENCARELLI A., MANNUCCI R., DISTRUTTI E., MORELLI A., DEL SOLDATO P. and MONCADA S., 2002. NCX-4016, a nitric oxide-releasing aspirin, protects endothelial cells against apoptosis by modulating mitochondrial function. FASEB J., 16, 1645-1647.
9. FIORUCCI S., SANTUCCI L., GRESELE P., FACCINO R.M., DEL SOLDATO P. and MORELLI A., 2003. Gastrointestinal safety of NO-aspirin (NCX 4016) in healthy human volunteers: a proof of concept endoscopic study. Gastroenterology, 124, 600-607.
10. FITZGERALD G.A., OATES J.A., HAWIGER J., MAAS R.L., ROBERTS L.J. II, LAWSON J.A. and BRASH A.R., 1983. Endogenous biosynthesis of prostacyclin and thromboxane and platelet function during chronic administration of aspirin in man. J. Clin. Invest., 71, 676-688.
11. FREDDUZZI S., MARIUCCI G., TANTUCCI M., DEL SOLDATO P. and AMBROSINI M.V., 2001. Nitro-aspirin (NCX 4016) reduces brain damage induced by focal cerebral ischemia in the rat. Neurosci. Lett., 302, 121-124.
12. FUKAO M., HATTORI Y., KANNO M., SAKUMA I. and KITABAKE A., 1997. Alterations in endothelium-dependent hyperpolarization and relaxation in mesenteric arteries from streptozotocin-induced diabetic rats. Br. J. Pharmacol., 121, 1287-1294.
13. FURCHGOTT R.F. and ZAWADZKI J.V., 1980. The obligatory role of endothelial cells in the relaxations of arterial smooth muscle by acetylcholine. Nature, 288, 373-376.
14. GABBIANI G., ELEMER G., GUELPA C.H., VALLOTTON M.B., BADONEL M.C. and HOTTNER I., 1979. Morphologic and functional changes of the aortic intima during experimental hypertension. Am. J. Pathol., 96, 399-422.
15. GARCIA-CARDEÑA G., COMANDER J., ANDERSON K.R., BLAKMAN B.R. and GIMBRONE M.A. JR., 2001. Biomechanical activation of vascular endothelium as a determinant of its functional phenotype. Proc. Natl. Acad. Sci. USA, 98, 4478-4485.
16. GIUGLIANO D., MARFELLA R., COPPOLA L., VERRAZZO G., ACAMPORA R., GIUNTA R., NAPPO F., LUCARELLI C. and D'ONOFRIO F., 1997. Vascular effects of acute hyperglycemia in human are reversed by L-arginine: evidence for reduced availability of nitric oxide during hyperglycemia. Circulation, 95, 1783-1790.
17. GOLIGORSKY M.S., CHEN J. and BRODSKY S., 2001. Endothelial cell disfunction leading to diabetic nephropathy: Focus on nitric oxide. Hypertension, 37, 744-748.
18. INGERMAN-WOJENSKI C.M., SEDAR A.W., NISSENBAUM M., SILVER M.J., KLURFELD D.M. and KRITCHEVSKY D., 1983. Early morphological changes in the endothelium of a peripheral artery of rabbits fed an atherogenic diet. Exp. Mol. Pathol., 38, 48-60.
19. LEE M.M.L., SCHUESSLER G.B. and CHIEN S., 1988. Time-dependent effects of endotoxin on the ultrastructure of aortic endothelium. Artery, 15, 71-89.
20. LEWIS J.C. and KOTTKE B.A., 1977. Endothelial damage and thrombocyte adhesion in pigeon atherosclerosis. Science, 196, 1007-1009.
21. MASUDA H., SHOZAWA T., KANDA M. and KAMIYA A., 1985. Endothelial surface of the blood flow loaded canine carotid artery. A scanning and transmission electron microscopy study. Acta Pathol. Jpn., 35, 1037-1046.
22. MATSUNAGA T., IGUCHI K., NAKAJIMA T., KOYAMA I., MIYAZAKI T., INOUE I., KAWAI S., KATAYAMA S., HIRANO K., HOKARI S. and KOMODA T., 2001. Glycated high-density lipoprotein induces apoptosis of endothelial cells via a mitochondrial dysfunction. Biochem. Biophys. Res. Commun., 287, 714-720.
23. MOTTA P., ANDREWS P.M. and PORTE K.R., 1984. Sistema vascolare sanguigno. In: 'Atlante di Microscopia Elettronica a Scansione'. Motta P., Andrews P.M., Porte K.R. eds., Vallardi Press, Milano, pp. 54-60.
24. NATHAN D.M., 1993. Long-term complications of diabetes mellitus. N. Engl. J. Med., 328, 1676-1685.
25. NELSON E., SUNAGA T., SHIMAMOTO T., KAWAMURA J., RENNELS M.L. and HEBEL R., 1975. Ischemic carotid endothelium. Scanning electron microscopical studies. Arch. Pathol., 99, 125-131.
26. PALMER R.M., FERRIGE A.G. and MONCADA S., 1987. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature, 327, 524-526.
27. PIEPER G.M., SIEBENEICH W., OLDS C.L., FELIX C.C. and DEL SOLDATO P., 2002. Vascular protective actions of a nitric oxide aspirin analog in both in vitro and in vivo models of diabetes mellitus. Free Radic. Biol. Med., 32, 1143-1156.
28. RADOMSKI M.W., PALMER R.M.J. and MONCADA S., 1987. The anti-aggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide. Br. J. Pharmacol., 92, 639-646.
29. RICHARDSON M. and PARBTANI A., 1987. Identification of non-denuding endothelial injury by scanning electron microscopy. Scanning Microsc., 1, 1315-1326.
30. RIVERO-VILCHES G.J., DE FRUTOS S., SAURA M., RODRIGUEZ-PUYOL D. and RODRIGUEZ-PUYOL M., 2003. Differential relaxing responses to particulate or soluble guanylyl cyclase activation on endothelial: a mechanism dependent on PKG-I alpha activation by NO/cGMP. Am. J. Physiol. Cell Physiol., 285, C891-C898.
31. ROSSI M.A., 1997. Aortic endothelial cell changes in the acute septicemic phase of experimental Trypanosoma Cruzi infection in rats: scanning and transmission electron microscopic study. Am. J. Trop. Med. Hyg., 57, 321-327.
32. ROSSONI G., BERTI M., DE COLONNA V.D., BERNAREGGI M., DEL SOLDATO P. and BERTI F., 2000. Myocardial protection by the nitroderivative of aspirin, NCX 4016: in vitro and in vivo experiments in the rabbit. Ital. Heart J., 1, 146-155.
33. RUBANI G.M., 1993. The role of endothelium in cardiovascular homeostasis and diseases. J. Cardiovasc. Pharmacol., 22, S1-S14.
34. SHIMOKATA H., 1999. Primary endothelial dysfunction: atherosclerosis. J. Mol. Cell. Cardiol. 31, 23-27.
35. SJÖGREN A. and EDVINSSON, L., 1988. Vasomotor changes in isolated coronary arteries from diabetic rats. Acta Physiol. Scand., 134, 429-436.
36. SKYRME-JONES R.A.P. and MEREDITH I.T., 2000. Aspirin does not improve endothelial vasodilator function in Type 1 diabetes mellitus. Diabet. Med., 17, 556-557.
37. STILL W.J. and DENNISON S., 1974. The arterial endothelium of the hypertensive rat: a scanning and transmission electron microscopical study. Arch. Pathol., 97, 337-342.
38. TAKEUCHI K., UKAWA H., KONAKA A., KITAMURA M. and SAGAWA Y., 1998. Effect of nitric-oxide-releasing aspirin derivative on gastric functional and ulcerogenic responses in rats: comparison with plain aspirin. J. Pharmacol. Exp. Ther., 286, 115-121.
39. TASHIMA K., FUJITA A., UMEDA M. and TAKEUCHI K., 2000. Lack of gastric toxicity of nitric-oxide releasing aspirin, NCX 4016, in the stomach of diabetic rats. Life Sci., 67, 1639-1652.
40. VAN DER OUDERAA F.J., BUYTENHEK M., NUGTEREN D.H. and VAN DORP D.A., 1980. Acetylation of prostaglandin endoperoxide synthase with acetylsalicylic acid. Eur. J. Biochem., 109, 1-8.
41. VANE J.R., ANGARD E.E. and BOTTING R.M., 1990. Regulatory function of vascular endothelium. N. Engl. J. Med., 323, 27-36.
42. WALLACE J.L., MCKNIGHT W., DEL SOLDATO P., BAYDOUN A.W. and CIRINO G., 1995. Anti-thrombotic effects of a nitric oxide-releasing, gastric-sparing aspirin derivative. J. Clin Invest., 96, 2711-2718.
43. WALLACE J.L., ELLIOT S.N., DEL SOLDATO P., MCKNIGHT W., SANNICOLO F. and CIRINO, G., 1997. Gastrointestinal-sparing anti-inflammatory drugs: the development of nitric oxide-releasing NSAIDs. Drug Dev. Res., 42, 144-149.