Comparative analysis of various in vitro COT kinase assay formats and their applications in inhibitor identification and characterization

Analytical Biochemistry

Volumn 350, Issue 2, 2006, Pages 268-276

  Jia, Yong ^a   Quinn, Christopher M ^a   Clabbers, Anca ^a   Talanian, Robert ^a   Xu, Yajun ^a,b   Wishart, Neil ^a   Allen, Hamish ^a  

^a ABBOTT LABORATORIES   (United States)

^b MILLENNIUM PHARMACEUTICALS INC   (United States)

Author keywords

Cascade assay; COT; Direct assay; HTRF; MAP kinase; MAP3 kinase; Radiometric; Tpl2

Indexed keywords

CELL DEATH; DIAGNOSIS; DRUG PRODUCTS; MACROPHAGES; MECHANICS; RADIOMETRY;

HTRF; MAP KINASE; MAP3 KINASE; RADIOMETRIC; TPL2;

ENZYMES;

ADENOSINE TRIPHOSPHATE; CANCER OZAKA THYROID ENZYME; ENZYME INHIBITOR; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE KINASE KINASE; PHOSPHORUS 33; PLUTONIUM 240; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ASSAY; ENZYME BINDING; ENZYME KINETICS; FLUORESCENCE ANALYSIS; IN VITRO STUDY; INTERMETHOD COMPARISON; PRIORITY JOURNAL; RADIOMETRY; SCREENING;

EID: 33644687467     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.11.010     Document Type: Article

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