Construction and expression of a single chain Fv fragment against pharmacologically active paeoniflorin in Escherichia coli, and its potential use in an enzyme-linked immunosorbent assay

Planta Medica

Volumn 72, Issue 2, 2006, Pages 151-155

  Lu, Zhaohua ^a   Masaki, Tadashi ^a   Shoyama, Yukihiro ^a   Tanaka, Hiroyuki ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Albiflorin; Enzyme linked immunosorbent assay (ELISA); Paeoniflorin; Single chain variable fragment (scFv)

Indexed keywords

ALBIFLORIN; COMPLEMENTARY DNA; MONOCLONAL ANTIBODY; PAEONIA EXTRACT; PAEONIFLORIN; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; CELL INCLUSION; CONTROLLED STUDY; CROSS REACTION; DNA SPLICING; DRUG BINDING; DRUG DETERMINATION; DRUG SYNTHESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; HEAVY CHAIN; HYBRIDOMA CELL CULTURE; LIGHT CHAIN; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION;

ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; BENZOIC ACIDS; BRIDGED COMPOUNDS; CELL LINE; CROSS REACTIONS; DRUGS, CHINESE HERBAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GLUCOSIDES; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; PAEONIA; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; PAEONIA;

EID: 33644519966     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2005-873188     Document Type: Article

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