Cloning and expression of a single-chain catalytic antibody that acts as a glutathione peroxidase mimic with high catalytic efficiency

Biochemical Journal

Volumn 359, Issue 2, 2001, Pages 369-374

  Ren, X ^a   Gao, S ^a   You, D ^a   Huang, H ^a   Liu, Z ^a   Mu, Y ^a   Liu, J ^a   Zhang, Y ^a   Yan, G ^a   Luo, G ^a   Yang, T ^a   Shen, J ^a  

^a JILIN UNIVERSITY   (China)

Author keywords

Artificial enzyme; Chemical mutation; Enzyme mimics; Protein engineering; Selenium

Indexed keywords

BACTERIA; BIODIVERSITY; CATALYSIS; CHROMATOGRAPHIC ANALYSIS; CLONING; ENZYMES; ESTERS; MUTAGENESIS; OXYGEN; PEROXIDES;

ABZYMES;

MONOCLONAL ANTIBODIES;

CATALYTIC ANTIBODY; GLUTATHIONE; GLUTATHIONE PEROXIDASE; HAPTEN; REACTIVE OXYGEN METABOLITE; RECOMBINANT ENZYME;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, CATALYTIC; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; CATALYTIC DOMAIN; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; GLUTATHIONE PEROXIDASE; KINETICS; MICE; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS; SELENIUM;

EID: 0035886938     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3590369     Document Type: Article

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