An intercalator film as a DNA-electrode interface

Chemical Communications

Volumn , Issue 9, 2006, Pages 962-964

  Taft, Bradford J ^a   Lapierre Devlin, Melissa A ^a   Kelley, Shana O ^a  

^a BOSTON COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ARTICLE; ELECTRODE; ELECTRON TRANSPORT; FILM; SURFACE PROPERTY;

EID: 33244488977     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/b515190b     Document Type: Article

References (27)

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24. 4- blocking electrochemistry assays were used to monitor DNA binding to EtX-modified electrodes (see ESI figure S2†).
25. Ethidium, which binds to ds DNA via intercalation, also binds to ss DNA electrostatically at high concentrations, hence its use as a ubiquitous gel electrophoresis stain for both structural forms (see J. Sambrook, E. F. Fritsch, and T. Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2nd edn, 1989). The high effective concentration of EtX within the immobilized film must therefore drive binding of ss DNA.
26. 2 caused dissociation. In addition, the introduction of a diluent into the film (e.g. benzenethiol) also decreased surface coverage of all types of DNA (data not shown).
27. 5 For the EtX wire system, the rate was too fast to be accurately measured using conventional electrochemistry.