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[6] [10] At least 0.1M NaCl was required in the buffer for the DNA modification to proceed in high yield, presumably to minimize the electrostatic interactions between the phosphate backbone of DNA and the nanocrystal ligand shell. Gold nanocrystals modified with less than 0.9 equivalents of ssDNA gave low yields of the molecules, whereas gold nanocrystals modified with more than 0.9 equivalents cross-linked to form aggregates that were larger than the desired molecules.
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33747569143
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Gel electrophoresis was a more reliable method for analyzing the number of ssDNAs per nanocrystal than UV spectroscopy due to the large extinction coefficient of the phosphane-complexed nanocrystals at 260 nm.
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