Polymerase chain reaction-ELISA to detect bacillus anthracis from soil samples - Limitations of present published primers

Journal of Applied Microbiology

Volumn 87, Issue 2, 1999, Pages 229-236

  Beyer, W ^a   Pocivalsek, S ^a   Bohm R ^a  

^a UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOLOGY; CHAINS; GENES; OLIGONUCLEOTIDES; PROBES; SOIL SURVEYS; SOILS;

BACILLUS ANTHRACIS; CHROMOSOMAL SEQUENCES; ENCODING GENES; GENOMIC DNA; MICROTITRE PLATE; SOIL MATERIALS; SOIL ORGANISMS; SOIL SAMPLE; TEST SENSITIVITY;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA; OLIGONUCLEOTIDE; STREPTAVIDIN;

ACCURACY; ARTICLE; BACILLUS ANTHRACIS; BACTERIUM ISOLATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE SEQUENCE; GENE TARGETING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; SOIL;

BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0032820963     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.1999.00875.x     Document Type: Article

References (12)

1. Beyer, W., Glockner, P., Otto, J. and Böhm, R. (1995) A nested PCR method for the detection of Bacillus anthracis in environmental samples collected from former tannery sites. Microbiological Research 150, 179-186.
2. Carl, M., Hawkins, R., Coulson, N., Lowe, J., Robertson, D.L., Nelson, W.M., Titball, R.W. and Woody, J.N. (1992) Detection of spores of Bacillus anthracis using the polymerase chain reaction. Journal of Infectious Disease 165, 1145-1148.
3. Johns, M., Harrington, L., Titball, R.W. and Leslic, D.L. (1994) Improved methods for the detection of Bacillus anthracis spores by the polymerase chain reaction. Letters in Applied Microbiology 18, 236-238.
4. Makino, S.-I., linuma-Okada, Y., Maruyama, T., Ezaki, T., Sasakawa, C. and Yoshikawa, M. (1993) Direct detection of Bacillus anthracis DNA in animals by polymerase chain reaction. Journal of Clinical Microbiology 31, 547-551.
5. Makino, S., Uchida, J., Terakado, N., Sasakawa, C. and Yoshikawa, M. (1989) Molecular characterisation and protein analysis of the cap region, which is essential for encapsulation in Bacillus anthracis. Journal of Bacteriology 171, 722-730.
6. Patra, G., Sylvester, P., Ramisse, V., Therasse, J. and Guesdon, J.L. (1996) Isolation of a specific chromosomic DNA sequence of Bacillus anthracis and its possible use in diagnosis. FEMS Immunol. Med. Microbiol. 672, 1-8.
7. Ramisse, V., Patra, G., Garrigue, H., Guesdon, J.-L. and Mock, M. (1996) Identification and characterisation of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pX01 and pX02 and chromosomal DNA. FEMS Microbiological Letters 145, 9-16.
8. Rasmussen, S.R., Larsen, M.R. and Rasmussen, S.E. (1991) Covalent immobilisation of DNA onto polystrene microwells: the molecules are bound at the 5' end. Analytical Biochemistry, 198, 138-142.
9. Reif, T.C., Johns, M., Pillai, S.D. and Carl, M. (1994) Identification of capsule-forming Becillus anthracis spores with the PCR and a novel dual-probe hybridisation format. Applied Environmental Microbiology 6, 1622-1625.
10. Sjöstedt, A., Eriksson, U., Ramisse, V. and Garrigue, H. (1997) Detection of Bacillus anthracis spores in still by PCR. FEMS Microbiological Ecology 23, 159-168.
11. Sjöstedt, A., Eriksson, U., Ramisse, V. and Garrigue, H. (1995) Detection of the vegetative form of Bacillus anthracis in soil by PCR. Salisbury Medical Bulletin Special Supplement 87, 50.
12. Titball, R.W., Turnbull, P.C.B. and Hutson, R.A. (1991) The monitoring and detection of Bacillus anthracis in the environment. Journal of Applied Bacteriology Symp Suppl 70, 95-185.