Retargeting serum immunoglobulin with bispecific diabodies

Nature Biotechnology

Volumn 15, Issue 7, 1997, Pages 632-636

  Holliger, Philipp ^a   Wing, Mark ^b   Pound, John D ^c   Bohlen, Heribert ^d   Winter, Greg ^a,e  

^a MRC CENTRE FOR PROTEIN ENGINEERING   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c UNIVERSITY DEPARTMENT OF MEDICINE   (United Kingdom)

^d UNIVERSITY OF COLOGNE   (Germany)

^e MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

Antibody engineering; Diabody; Immunotherapy

Indexed keywords

BISPECIFIC ANTIBODY; CD8 ANTIGEN; COMPLEMENT; IMMUNOGLOBULIN; LYSOZYME; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANTIBODY DEPENDENT CELLULAR CYTOTOXICITY; ARTICLE; CONTROLLED STUDY; HEMAGGLUTINATION; HEMOLYSIS; HUMAN; HUMAN CELL; IMMUNOGLOBULIN BLOOD LEVEL; MONONUCLEAR CELL; NONHUMAN; PHAGOCYTOSIS; PRIORITY JOURNAL; RESPIRATORY BURST; ROSETTE FORMATION; SHEEP ERYTHROCYTE;

ANIMALS; ANTIBODIES, BISPECIFIC; ANTIGENS; BASE SEQUENCE; BIOTECHNOLOGY; COMPLEMENT SYSTEM PROTEINS; CYTOTOXICITY, IMMUNOLOGIC; DNA PRIMERS; HALF-LIFE; HUMANS; IMMUNIZATION, PASSIVE; IMMUNOGLOBULINS; MURAMIDASE; PHAGOCYTOSIS; RECEPTORS, IGG; TUMOR CELLS, CULTURED;

ANIMALIA; OVIS ARIES;

EID: 0030796062     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt0797-632     Document Type: Article

References (35)

1. Waldman, T.A. 1991. Monoclonal antibodies in diagnosis and therapy. Science 252:1657-1662.
2. Neuberger, M.S., Williams, G.T., Mitchell, E.B., Jouhal, S.S., Flanagan, J.G., and Rabbitts, T.H. 1985. A hapten-specific chimeric IgE antibody with human physiological effector function. Nature 314:268-270.
3. Jones, P.T., Dear, P.H., Foote, J., Neuberger, M.S., and Winter, G. 1986. Replacing the complementarity-determining regions in a human antibody with those from a mouse. Nature 321:522-525.
4. Riechmann, L, Clark, M., Waldmann, H., and Winter, G. 1988. Reshaping human antibodies for therapy. Nature 332:323-327.
5. Nose, M. and Wigzell, H. 1983. Biological significance of carbohydrate chains on monoclonal antibodies. Proc. Watt Acad. Sci. USA 80:6632-6636.
6. Walker, M.R., Lund, J., Thompson, K.M., and Jefferis, R. 1989. Aglycosilation of human lgG1 and lgG3 monoclonal antibodies can eliminate recognition by human cells expressing FcγRI and FcγRIII receptors. Biochem. J. 259:347-352.
7. Winter, G., Griffiths, A.D., Hawkins, R.E., and Hoogenboom, H.R. 1994. Making antibodies by phage display technology. Annu. Rev. Immunol. 12:433-455.
8. Cumber, A.J., Ward, E.S., Winter, G., Parnell, G.D., and Wawrzynczak, E.J. 1992. Comparative stabilities in vitro and in vivo of a recombinant mouse antibody FvCys fragment and a bisFvCys conjugate. J. Immunol. 149:120-126.
9. King, D.J., Turner, A., Farnsworth, A.P.H., Adair, J.R., Owens, R.J., Pedley, R.B., et al. 1994. Improved tumor targeting with chemically cross-linked recombinant antibody fragments. Cancer Res. 54:6176-6185.
10. Neuberger, M.S., Williams, G.T., and Fox, R.O. 1984. Recombinant antibodies possessing novel effector functions. Nature 312:604-648.
11. Holliger, P. and Winter, G. 1993. Engineering bispecific antibodies. Curr. Op. Biotech. 4:446-449.
12. Peresics, O., Webb, P.A., Holliger, P., Winter, G., and Williams, R.L. 1994. Crystal structure of a diabody, a bivalent antibody fragment. Structure 2:1218-1226.
13. Holliger, P., Prospero, T., and Winter, G. 1993. “Diabodies”: small bivalent and bispecific antibody fragments. Proc. Natl. Acad. Sci. USA 90:6444-6448.
14. McIntire, K.R. and Rouse, A.M. 1970. Mouse immunoglobulin light chains: alterations of the κ:λ ratio. Fed. Proc. Fed. Am. Soc. Exp. Biol. 29:704-709.
15. Pound, J.D., Lund, J., and Jefferis, R. 1993. Human FcyRI triggering of the mononuclear phagocyte respiratory burst. Molec. Immunol. 30:469-478.
16. Tomlinson, S. 1993. Complement defense mechanisms. Curr. Op. Immunol. 5:83-89.
17. Holliger, P., Brissinck, J., Williams, R.L., Thielemans, K., and Winter, G. 1996. Specific killing of lymphoma cells by cytotoxic T-cells mediated by a bispecific diabody. Protein Eng. 9:299-305.
18. Griffiths, A.D., Williams, S.C., Hartley, O., Tomlinson, I.M., Waterhouse, P., Crosby, W.L., et al. 1994. Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 13:3245-3260.
19. Brüggemann, M., Winter, G., Waldmann, H., and Nyeberger, M.S. 1989. The Immunogeniclty of chimeric antibodies. J. Exp. Med. 170:2153-2157.
20. Perelson, A.S. 1989. Immune network theory. Immunol. Rev. 110:5-36.
21. Yokota, T., Milenic, D.E., Whitlow, M., and Schlom, J. 1992. Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms. Cancer Res. 52:3402-3408.
22. Marks, J.D., Griffiths, A.D., Malmqvist, M., Clackson, T, Bye, J.M., and Winter, G. 1992. By-passing immunization: Building high affinity human antibodies by chain shuffling. Bio/Technology 10:779-783.
23. Low, N., Holliger, P., and Winter, G. 1996. Mimicking somatic hypermutation: Affinity maturation of antibodies displayed on bacteriophage using a bacterial mutator strain. J. Mol. Biol. 260:359-368.
24. Hoogenboom, H.R., Griffiths, AD., Johnson, K.S., Chiswell, D.J., Hudson, R, and Winter, G. 1991. Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19:4133-4137.
25. Reth, M., Imanishi-Kari, X, and Rajewski, K. 1979. Analysis of the repertoire of anti(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies in C57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur. J. Immunol. 9:1004-1013.
26. L expression by monoclonal antibodies recognizing avian lysozyme. J. Immunol. 132:963-967.
27. Chester, KA, Begent, R.H., Robson, L, Keep, P., Pedley, R.B., Boden, J.A, et al. 1994. Phage libraries for generation of clinically useful antibodies. Lancet 343:455-456.
28. Orlandi, R., Gussow, D.H., Jones, P.T., and Winter, G. 1989. Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 86:3833-3837.
29. Clackson, T., Hoogenboom, H.R., Griffiths, A.D., and Winter, G. 1991. Making antibody fragments using phage display libraries. Nature 352:624-628.
30. Ward, E.S., Gussow, D., Griffiths, A.D., Jones, P.T., and Winter, G. 1989. Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli. Nature 341:544-546.
31. McGuiness, B.X, Walter, G., Fitzgerald, K., Schuler, P., Mahoney, W., Duncan, A.R., et al. 1996. Phage diabody repertoires for selection of large numbers of bispecific antibody fragments. Nature Biotechnology 14:1149-1154.
32. Pound, J.D., Lund, J., and Jefferis, R. 1993. Aglycosilated chimaeric human lgG3 can trigger the human phagocyte respiratory burst. Mol. Immunol. 30:233-241.
33. 3 derivatives that use cooperative signaling via the TCR/CD3 complex and CD2 to activate and redirect resting cytotoxic T cells. J. Immunol. 147:60-69.
34. Bohlen, H., Manzke, O., Engert, A., Hertel, M., Hippler, Altenburg, R., et al. 1994. Differentiation of cytotoxicity using target cells labelled with europium and samarium by electroporation. J. Immunol. Methods 173:55-62.
35. Neri, D., Prospero, T., Petral, H., Winter, G., Browne, M., and Vanderpant, L. 1996. Multipurpose High Sensitivity Luminescence Analyzer (LUANA): Use in gel electrophoresis. Bio Techniques 20:708-712.