A novel yeast-based reporter assay system for the sensitive detection of genotoxic agents mediated by a DNA damage-inducible LexA-GAL4 protein

Journal of Biochemistry

Volumn 139, Issue 1, 2006, Pages 105-112

  Ichikawa, Kohei ^a   Eki, Toshihiko ^a  

^a TOYOHASHI UNIVERSITY OF TECHNOLOGY   (Japan)

Author keywords

DNA damage; Genotoxicity test; LexA GAL4 fusion protein; Sensitive reporter assay; Yeast mutant

Indexed keywords

ALKYLATING AGENT; BETA GALACTOSIDASE; CAMPTOTHECIN; LEXA PROTEIN; TRANSCRIPTION FACTOR GAL4;

ARTICLE; CELL WALL; CONTROLLED STUDY; DNA BINDING; DNA DAMAGE; DNA REPAIR; ENZYME ACTIVITY; ENZYME INDUCTION; ESCHERICHIA COLI; GENOTOXICITY; NONHUMAN; PROTEIN BINDING; TRANSCRIPTION INITIATION; YEAST;

BACTERIAL PROTEINS; BASE SEQUENCE; DNA DAMAGE; DNA PRIMERS; DNA-BINDING PROTEINS; GENES, REPORTER; MUTAGENICITY TESTS; MUTAGENS; SACCHAROMYCES CEREVISIAE PROTEINS; SENSITIVITY AND SPECIFICITY; SERINE ENDOPEPTIDASES; TRANSCRIPTION FACTORS;

ESCHERICHIA COLI;

EID: 32944482094     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvj011     Document Type: Article

References (38)

1. Friedberg, E.C., Walker, G., and Siede, W. (1995) DNA Repair and Mutagenesis, American Society for Microbiology Press, Washington, DC
2. Ames, B.N., Durston, W.E., Yamasaki, E., and Lee, F.D. (1973) Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc. Natl. Acad. Sci. USA 70, 2281-2285
3. Quillardet, P., Huisman, O., D'Ari, R., and Hofnung, M. (1982) SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity. Proc. Natl. Acad. Sci. USA 79, 5971-5975
4. Oda, Y., Nakamura, S., Oki, I., Kato, T., and Shinagawa, H. (1985) Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens. Mutat. Res. 147, 219-229
5. Walmsley, R.M., Billinton, N., and Heyer, W.D. (1997) Green fluorescent protein as a reporter for the DNA damage-induced gene RAD54 in Saccharomyces cerevisiae. Yeast 13, 1535-1545
6. Afanassiev, V., Sefton, M., Anantachaiyong, T., Barker, G., Walmsley, R., and Wolfl, S. (2000) Application of yeast cells transformed with GFP expression constructs containing the RAD54 or RNR2 promoter as a test for the genotoxic potential of chemical substances. Mutat. Res. 464, 297-308
7. Jia, X., Zhu, Y., and Xiao, W. (2002) A stable and sensitive genotoxic testing system based on DNA damage induced gene expression in Saccharomyces cerevisiae. Mutat. Res. 519, 83-92
8. Jelinsky, S.A. and Samson, L.D. (1999) Global response of Saccharomyces cerevisiae to an alkylating agent. Proc. Natl. Acad. Sci. USA 96, 1486-1491
9. Gasch, A.P., Spellman, P.T., Kao, C.M., Carmel-Harel, O., Eisen, M.B., Storz, G., Botstein, D., and Brown, P.O. (2000) Genomic expression programs in the response of yeast cells to environmental changes. Mol. Biol. Cell 11, 4241-4257
10. Quillardet, P. and Hofnung, M. (1987) Induction of the SOS system in a dam-3 mutant: a diagnostic strain for chemicals causing DNA mismatches. Mutat. Res. 177, 17-26
11. Jia, X. and Xiao, W. (2003) Compromised DNA repair enhances sensitivity of the yeast RNR3-lacZ genotoxicity testing system. Toxicol. Sci. 75, 82-88
12. Winzeler, E.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J.D., Bussey, H., Chu, A.M., Connelly, C., Davis, K., Dietrich, F., Dow, S.W., El Bakkoury, M., Foury, F., Friend, S.H., Gentalen, E., Giaever, G., Hegemann, J.H., Jones, T., Laub, M., Liao, H., Liebundguth, N., Lockhart, D.J., Lucau-Danila, A., Lussier, M., M'Rabet, N., Menard, P., Mittmann, M., Pai, C., Rebischung, C., Revuelta, J.L., Riles, L., Roberts, C.J., Ross-MacDonald, P., Scherens, B., Snyder, M., Sookhai-Mahadeo, S., Storms, R.K., Veronneau, S., Voet, M., Volckaert, G., Ward, T.R., Wysocki, R., Yen, G.S., Yu, K, Zimmermann, K., Philippsen, P., Johnston, M., and Davis, R.W. (1999) Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285, 901-906
13. Burke, D., Dawson, D., and Stearns, T. (2000) Methods in Yeast Genetics, Cold Spring Harbor Laboratory Press, New York
14. Gietz, D., St Jean, A., Woods, R.A., and Schiestl, R.H. (1992) Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 20, 1425
15. Myers, A.M., Tzagoloff, A., Kinney, D.M., and Lusty, C.J. (1986) Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45, 299-310
16. Iha, H. and Tsurugi, K. (1998) Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques 25, 936-938
17. Golemis, E.A., Serebriiskii, I., Finley, R.L., Kolonin, M.G., Gyuris, J., and Brent, R. (1999) Interaction trap/two-hybrid system to identify interacting proteins. In Current Protocols in Molecular Biology (F.M. Ausubel et al., eds.), Section. 20.1, pp. 1-40, John Wiley & Sons, Inc.
18. Estojak, J., Brent, R., and Golemis, E.A. (1995) Correlation of two-hybrid affinity data with in vitro measurements. Mol. Cell. Biol. 15, 5820-5829
19. Griffith, K.L. and Wolf, R.E., Jr. (2002) Measuring β-galactosidase activity in bacteria: cell growth, permeabilization, and enzyme assays in 96-well arrays. Biochem. Biophys. Res. Commun. 290, 397-402
20. Brouchon-Macari, L., Joseph, M.C., and Dagher, M.C. (2003) Highly sensitive microplate β-galactosidase assay for yeast two-hybrid systems. Biotechniques 35, 446-448
21. Gasch, A.P. and Werner-Washburne, M. (2002) The genomics of yeast responses to environmental stress and starvation. Fund. Integr. Genomics 2, 181-192
22. Jelinsky, S.A., Estep, P., Church, G.M., and Samson, L.D. (2000) Regulatory networks revealed by transcriptional profiling of damaged Saccharomyces cerevisiae cells: Rpn4 links base excision repair with proteasomes. Mol. Cell. Biol. 20, 8157-8167
23. Elledge, S.J. and Davis, R.W. (1989) DNA damage induction of ribonucleotide reductase. Mol. Cell. Biol. 9, 4932-4940
24. Elledge, S.J., Zhou, Z., Allen, J.B., and Navas, T.A. (1993) DNA damage and cell cycle regulation of ribonucleotide reductase. Bioessays 15, 333-339
25. Gaber, R.F., Copple, D.M., Kennedy, B.K., Vidal, M., and Bard, M. (1989) The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol. Cell. Biol. 9, 3447-3456
26. de Bettignies, G., Barthe, C., Morel, C., Peypouquet, M.F., Doignon, F., and Crouzet, M. (1999) RGD1 genetically interacts with MID2 and SLG1, encoding two putative sensors for cell integrity signalling in Saccharomyces cerevisiae. Yeast 15, 1719-1731
27. Leduc, A., He, C.H., and Ramotar, D. (2003) Disruption of the Saccharomyces cerevisiae cell-wall pathway gene SLG1 causes hypersensitivity to the antitumor drug bleomycin. Mol. Genet. Genomics 269, 78-89
28. Hartwell, L.H., Szankasi, P., Roberts, C.J., Murray, A.W., and Friend, S.H. (1997) Integrating genetic approaches into the discovery of anticancer drugs. Science 278, 1064-1068
29. Simon, J.A., Szankasi, P., Nguyen, D.K., Ludlow, C., Dunstan, H.M., Roberts, C.J., Jensen, E.L., Hartwell, L.H., and Friend, S.H. (2000) Differential toxicities of anticancer agents among DNA repair and checkpoint mutants of Saccharomyces cerevisiae. Cancer Res. 60, 328-333
30. Morita, T., Iwamoto, Y., Shimizu, T., Masuzawa, T., and Yanagihara, Y. (1989) Mutagenicity tests with a permeable mutant of yeast on carcinogens showing false-negative in Salmonella assay. Chem. Pharm. Bull. (Tokyo). 37, 407-409
31. Staleva, L., Waltscheva, L., Golovinsky, E., and Venkov, P. (1996) Enhanced cell permeability increases the sensitivity of a yeast test for mutagens. Mutat. Res. 370, 81-89
32. Emter, R., Heese-Peck, A., and Kralli, A. (2002) ERG6 and PDR5 regulate small lipophilic drug accumulation in yeast cells via distinct mechanisms. FEBS Lett. 521, 57-61
33. Higgins, V.J., Alic, N., Thorpe, G.W., Breitenbach, M., Larsson, V., and Dawes, I.W. (2002) Phenotypic analysis of gene deletant strains for sensitivity to oxidative stress. Yeast 19, 203-214
34. Lussier, M., White, A.M., Sheraton, J., di Paolo, T., Treadwell, J., Southard, S.B., Horenstein, C.I., Chen-Weiner, J., Ram, A.F., Kapteyn, J.C., Roemer, T.W., Vo, D.H., Bondoc, D.C., Hall, J., Zhong, W.W., Sdicu, A.M., Davies, J., Klis, F.M., Robbins, P.W., and Bussey, H. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147, 435-450
35. Cahill, P.A., Knight, A.W., Billinton, N., Barker, M.G., Walsh, L., Keenan, P.O., Williams, C.V., Tweats, D.J., and Walmsley, R.M. (2004) The GreenScreen® genotoxicity assay: a screening validation programme. Mutagenesis 19, 105-119
36. Quillardet, P. and Hofnung, M. (1993) The SOS chromotest: a review. Mutat. Res. 297, 235-27
37. Xiao, W., Nowak, M., Laferte, S., and Fontanie, T. (1996) Mutagenicity and toxicity of the DNA alkylation carcinogens 1,2-dimethylhydrazine and azoxymethane in Escherichia coli and Salmonella typhimurium. Mutagenesis 11, 241-245
38. Olivier, P. and Marzin, D. (1987) Study of the genotoxic potential of 48 inorganic derivatives with the SOS chromotest. Mutat. Res. 189, 263-269