Interaction trap/two-hybrid system to identify interacting proteins

Current Protocols in Molecular Biology

Volumn , Issue SUPPL. 82, 2008, Pages

  Golemis, Erica A ^a   Serebriiskii, Ilya ^a   Finley Jr , Russel L ^b   Kolonin, Mikhail G ^b   Gyuris, Jeno ^c   Brent, Roger ^d  

^a FOX CHASE CANCER CENTER   (United States)

^b WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c GPC Biotech Inc   (United States)

^d The Molecular Sciences Institute   (United States)

Author keywords

Interaction; Interaction trap; Mating; Protein interactions; Yeast two hybrid

Indexed keywords

AMPICILLIN; BETA GALACTOSIDASE; CHLORAMPHENICOL; KANAMYCIN; LEXA PROTEIN; TRANSCRIPTION FACTOR GAL4;

ANTIBIOTIC RESISTANCE; COLONY FORMATION; CULTURE MEDIUM; DNA BINDING; DNA LIBRARY; DNA SCREENING; FUNGAL GENE; FUNGAL STRAIN; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DATABASE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; REVIEW; TRANSCRIPTION INITIATION; TWO HYBRID SYSTEM; YEAST;

EID: 57549112755     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb2001s82     Document Type: Review

References (31)

1. Bartel, P.L., Chien, C.-T., Sternglanz, R., and Fields, S. 1993. Using the two-hybrid system to detect protein-protein interactions. In Cellular Interactions in Development:APractical Approach (D.A. Hartley, ed.) pp. 153-179. Oxford University Press, Oxford.
2. Bartel, P.L., Roecklein, J.A., SenGupta, D., and Fields, S. 1996. A protein linkage map of Escherichia coli bacteriophage T7. Nature Genet. 12:72-77.
3. Bendixen, C., Gangloff, S., and Rothstein, R. 1994. A yeast mating-selection scheme for detection of protein-protein interactions. Nucleic Acids Res. 22:1778-1779.
4. Brent, R. and Ptashne, M. 1984. A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene. Nature 312:612-615.
5. Brent, R. and Ptashne, M. 1985. A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell 43:729-736.
6. Chiu,M.I., Katz, H., and Berlin, V. 1994. RAPT1, a mammalian homolog of yeast Tor, interacts with the FKBP12/rapamycin complex. Proc. Natl. Acad. Sci. U.S.A. 91:12574-12578.
7. Colas, P.,Cohen, B., Jessen, T.,Grishina, I.,McCoy, J., and Brent, R. 1996. Genetic selection of peptide aptamers that recognize and inhibit cyclindependent kinase 2. Nature 380:548-550.
8. Estojak, J., Brent, R., and Golemis, E.A. 1995. Correlation of two-hybrid affinity data with in vitro measurements. Mol. Cell. Biol. 15:5820-5829.
9. Fields, S. and Song, O. 1989. A novel genetic system to detect protein-protein interaction. Nature 340:245-246.
10. Finley, R.L. Jr. and Brent, R. 1994. Interaction mating reveals binary and ternary connections between Drosophila cell cycle regulators. Proc. Natl. Acad. Sci. U.S.A. 91:12980-12984.
11. Gietz, D., St. Jean, A., Woods, R.A., and Schiestl, R.H. 1992. Improvedmethod for high-efficiency transformation of intact yeast cells. Nucleic Acids Res. 20:1425.
12. Golemis, E.A. and Brent, R. 1992. Fused protein domains inhibit DNA binding by LexA. Mol. Cell Biol. 12:3006-3014.
13. Grunstein, M. and Hogness, D.S. 1975. Colony hybridization: Amethod for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. U.S.A. 72:3961-3965.
14. Gyuris, J., Golemis, E.A., Chertkov, H., and Brent, R. 1993. Cdi1, a human G1- and S-phase protein phosphatase that associates with Cdk2. Cell 75:791-803.
15. Izumchenko, E., Wolfson, M., Golemis, E.A., and Serebriiskii, I.G. 2007. Yeast hybrid approaches. In Yeast Gene Analysis (I. Stansfield and M. Stark, eds.) pp. 103-137. Elsevier Ltd., London.
16. Kaiser, C., Michaelis, S., and Mitchell, A. 1994. Methods in Yeast Genetics, a Cold Spring Harbor Laboratory Course Manual, pp.135-136. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
17. Kolonin, M.G. and Finley, R.L., Jr. 1998. Targeting cycling-dependent kinases in Drosophilia with peptide aptamers. Proc. Natl. Acad. Sci. U.S.A. 95:14266-14271.
18. Licitra, E.J. and Liu, J.O. 1996. A three-hybrid system for detecting small ligand-protein receptor interactions. Proc. Natl. Acad. Sci. U.S.A. 93:12817-12821.
19. Ma, J. and Ptashne, M. 1987. A new class of yeast transcriptional activators. Cell 51:113-119.
20. Ma, J. and Ptashne,M. 1988. Converting an eukaryotic transcriptional inhibitor into an activator. Cell 55:443-446.
21. Osborne, M., Dalton, S., and Kochan, J.P. 1995. The yeast tribrid system: Genetic detection of trans-phosphorylated ITAM-SH2 interactions. Bio/Technology 13:1474-1478.
22. Ruden, D.M.,Ma, J., Li, Y.,Wood, K., and Ptashne, M. 1991. Generating yeast transcriptional activators containing no yeast protein sequences. Nature 350:426-430.
23. Samson, M.-L., Jackson-Grusby, L., and Brent, R. 1989. Gene activation and DNA binding by Drosophila Ubx and abd-A proteins. Cell 57:1045-1052.
24. Schiestl, R.H. and Gietz, R.D. 1989. High-efficiency transformation of intact yeast cells using single-stranded nucleic acids as a carrier. Curr. Genet. 16:339-346.
25. SenGupta, D.J., Zhang, B., Kraemer, B., Pochart, P., Fields, S., and Wickens, M. 1996. A threehybrid system to detect RNA-protein interactions in vivo. Proc. Natl. Acad. Sci. U.S.A. 93:8496-8501.
26. Serebriiskii, I.G., Khazak, V., and Golemis, E.A. 1999. A two- hybrid dual bait system to discriminate specificity of protein interactions. J. Biol. Chem. 274:17080-17087.
27. Stagljar, I., Bourquin, J.-P., and Schaffner, W. 1996. Use of the two-hybrid system and random sonicated DNA to identify the interaction domain of a protein. BioTechniques 21:430-432.
28. Wang, Z.F., Whitfield, M.L., Ingledue, T.C. 3rd, Dominski,A., andMarzluff,W.F. 1996. The protein that binds the 3′ end of histone mRNA: A novel RNA-binding protein required for histone pre-mRNA processing. Genes & Dev. 10:3028-3040.
29. Watson, M.A., Buckholz, R., and Weiner, M.P. 1996. Vectors encoding alternative antibiotic resistance for use in the yeast two-hybrid system. BioTechniques 21:255-259.
30. West, R.W.J., Yocum, R.R., and Ptashne, M. 1984. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: Location and function of the upstream activator sequence UASG. Mol. Cell Biol. 4:2467-2478.
31. Yang, M., Wu, Z., and Fields, S. 1995. Proteinpeptide interactions analyzed with the yeast two-hybrid system. Nucleic Acids Res. 23:1152-1156.