Measuring β-galactosidase activity in bacteria: Cell growth, permeabilization, and enzyme assays in 96-well arrays

Biochemical and Biophysical Research Communications

Volumn 290, Issue 1, 2002, Pages 397-402

  Griffith, Kevin L ^a   Wolf Jr , Richard E ^a  

^a UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

Author keywords

96 well arrays; Gene regulation; Microtiter; Miller units; Plate reader; galactosidase assay

Indexed keywords

CHLOROFORM; GALACTOSIDASE;

ARTICLE; BACTERIAL GROWTH; CELL PERMEABILIZATION; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; MOLECULAR GENETICS; NONHUMAN; PRIORITY JOURNAL;

ACRONICTA LEPORINA; BACTERIA (MICROORGANISMS);

EID: 0036291504     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.6152     Document Type: Article

References (7)

1. A microtiter plate-based system for the semi-automated growth and assay of bacterial cells for β-galactosidase activity
2. cis-acting ompF mutations that result in OmpR-dependent constitutive expression
3. Automated kinetic assay of β-galactosidase activity
4. Automated determination of β-galactosidase specific activity
5. Rapid bacterial permeabilization reagent useful for enzyme assays
6. Genetic definition of the Escherichia coli zwf"soxbox," the DNA binding site for SoxS-mediated induction of glucose 6-phosphate dehydrogenase in response to superoxide