Expression of active human C1 inhibitor serpin domain in Escherichia coli

Protein Expression and Purification

Volumn 22, Issue 2, 2001, Pages 349-358

  Lamark, Trond ^a   Ingebrigtsen, Monica ^a   Bjørnstad, Camilla ^a   Melkko, Tarja ^b   Mollnes, Tom E ^a,c   Nielsen, Erik W ^a,d  

^a UNIVERSITY OF TROMSØ   (Norway)

^b UNIVERSITY OF OULU   (Finland)

^c Transfusion Medicine

^d NORDLAND HOSPITAL   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL SEQUENCE; CHAPERONIN; COMPLEMENT COMPONENT C1S INHIBITOR; COMPLEX FORMATION; CYTOPLASM; DISULFIDE BOND; GENETIC STRAIN; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEINASE; SERINE PROTEINASE INHIBITOR;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034952819     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1445     Document Type: Article

References (26)

1. C1-esterase inhibitor: An anti-inflammatory agent and its potential use in the treatment of diseases other than hereditary angioedema
2. Human C1 inhibitor: Primary structure, cDNA cloning, and chromosomal localization
3. Structure of a serpin-protease complex shows inhibition by deformation
4. The role of sialic acid in the functional activity and the hepatic clearance of C1-INH
5. Functional analysis of the serpin domain of C1 inhibitor
6. The native metastable fold of C1-inhibitor is stabilized by disulfide bonds
7. Mechanism of serpin action: Evidence that C1 inhibitor functions as a suicide substrate
8. Pathogenetic and clinical aspects of C1 inhibitor deficiency
9. The effect of C1-esterase inhibitor in definite and suspected streptococcal toxic shock syndrome. Report of seven patients
10. C1-inhibitor attenuates hyperacute rejection and inhibits complement, leukocyte and platelet activation in an ex vivo pig-to-human perfusion model
11. Rapid recovery of DNA from agarose gels
12. High efficiency transformation of Escherichia coli with plasmids
13. Expression of functional human C1 inhibitor in COS cells
14. The sequence of a cDNA encoding functional murine C1-inhibitor protein
15. Effect of time, temperature and additives on a functional assay of C1 inhibitor
16. A neoepitope-based enzyme immunoassay for quantification of C1-inhibitor complex with C1r and C1s
17. Quantification of plasma factor XIIa-C1-inhibitor and kallikrein-C1-inhibitor complexes in sepsis
18. Use of T7 RNA polymerase to direct expression of cloned genes
19. Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli
20. Effect of the codon following the ATG start site on the expression of ovine growth hormone in Escherichia coli
21. Molecular chaperones, folding catalysts, and the recovery of active recombinant proteins from E. coli. To fold or to refold
22. Purified chaperonin 60 (groEL) interacts with the nonnative states of a multitude of Escherichia coli proteins
23. The crystal structure of a GroEL/peptide complex: Plasticity as a basis for substrate diversity
24. 1-anti-trypsin expressed as inclusion bodies in Escherichia coli: Characterization of aggregation