A continuous-flow polymerase chain reaction microchip with regional velocity control

Journal of Microelectromechanical Systems

Volumn 15, Issue 1, 2006, Pages 223-236

  Li, Shifeng ^a   Fozdar, David Y ^a   Ali, Mehnaaz F ^b   Li, Hao ^c   Shao, Dongbing ^a   Vykoukal, Daynene M ^d   Vykoukal, Jody ^d   Floriano, Pierre N ^b   Olsen, Michael ^c   McDevitt, John T ^b   Gascoyne, Peter R C ^d   Chen, Shaochen ^a  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^c Iowa State University of Science and Technology   (United States)

^d UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

Continuous flow; Microelectromechanical systems (MEMS); Polymerase chain reaction (PCR); Regional velocity control

Indexed keywords

CONFINED FLOW; FINITE ELEMENT METHOD; HEAT TRANSFER; NUCLEATION; OPTIMIZATION; SERPENTINE; VELOCITY CONTROL; VELOCITY MEASUREMENT;

BUBBLE NUCLEATION; CONTINUOUS-FLOW; FLOW RECIRCULATION; MICRO PARTICLE IMAGE VELOCIMETRY; POLYMERASE CHAIN REACTION (PCR); REGIONAL VELOCITY CONTROL; SERPENTINE MICROCHANNEL;

DNA;

EID: 32244433373     PISSN: 10577157     EISSN: None     Source Type: Journal    
DOI: 10.1109/JMEMS.2005.859083     Document Type: Article

References (27)

1. K. B. Mullis, "The polymerase chain-reaction (nobel lecture)," Angewandte Chemie-International Edition in English, vol. 33, pp. 1209-1213, 1994.
2. C. T. Wittwer, K. M. Ririe, R. V. Andrew, D. A. David, R. A. Gundry, and U. J. Balis, "The lightcycler(TM) a microvolume multisample fluorimeter with rapid temperature control," Biotechniques, vol. 22, pp. 176-181, 1997.
3. Y. C. Lin, M. Y. Huang, K. C. Young, T. T. Chang, and C. Y. Wu, "A rapid micro-polymerase chain reaction system for hepatitis C virus amplification," Sensors and Actuators B-Chemical, vol. 71, pp. 2-8, 2000.
4. J.W. Hong, T. Fujii, M. Seki, T. Yamamoto, and I. Endo, "Integration of gene amplification and capillary gel electrophoresis on a polydimethylsiloxane-glass hybrid microchip," Electrophoresis, vol. 22, pp. 328-333, 2001.
5. E. T. Lagally, C. A. Emrich, and R. A. Mathies, "Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis," Lab. on a Chip, vol. 1, pp. 102-107, 2001.
6. Y. S. Shin, K. Cho, S. H. Lim, S. Chung, S. J. Park, C. Chung, D. C. Han, and J. K. Chang, "PDMS-based micro PCR chip with parylene coating," J. Micromechan. Microeng., vol. 13, pp. 768-774, 2003.
7. S. Li and S. Chen, "Design, simulation, and micro-fabrication of integrated, heat-conduction based DNA chip," J. Manufact. Process., vol. 6, pp. 24-31, 2003.
8. Z. Y. Chen, S. Z. Qian, W. R. Abrams, D. Malamud, and H. H. Bau, "Thermosiphon-based PCR reactor: Experiment and modeling," Anal. Chem., vol. 76, pp. 3707-3715, 2004.
9. M. Hashimoto, P. C. Chen, M. W. Michell, D. E. Nikitopoulos, S. A. Soper, and M. C. Murphy, "Rapid PCR in a continuous flow device," Lab. on a Chip, vol. 4, pp. 638-645, 2004.
10. I. Schneega and J. M. Kohler, "Flow-through polymerase chain reactions in chip thermocyclers," Rev. Mole. Biotechnol., vol. 82, pp. 101-121, 12 2001.
11. I. Schneegass, R. Brautigam, and J. M. Kohler, "Miniaturized flow-through PCR with different template types in a silicon chip thermocycler," Lab. on a Chip, vol. 1, pp. 42-49, 2001.
12. C. F. Chou, R. Changrani, P. Roberts, D. Sadler, J. Burdon, F. Zenhausern, S. Lin, A. Mulholland, N. Swami, and R. Terbrueggen, "A miniaturized cyclic PCR device-modeling and experiments," Microelectron. Eng., vol. 61, no. 2, pp. 921-925, 2002.
13. J. Liu, M. Enzelberger, and S. Quake, "A nanoliter rotary device for polymerase chain reaction," Electrophoresis, vol. 23, pp. 1531-1536, 2002.
14. M. U. Kopp, A. J. de Mello, and A. Manz, "Chemical amplification: Continuous-flow PCR on a chip," Science, vol. 280, pp. 1046-1048, 1998.
15. P. J. Obeid, T. K. Christopoulos, H. J. Crabtree, and C. J. Backhouse, "Microfabricated device for DNA and RNA amplification by continuous-flow polymerase chain reaction and reverse transcription-polymerase chain reaction with cycle number selection," Anal. Chem., vol. 75, pp. 288-295, 2003.
16. D. S. Yoon, Y. S. Lee, Y. Lee, H. J. Cho, S. W. Sung, K. W. Oh, J. Cha, and G. Lim, "Precise temperature control and rapid thermal cycling in a micromachined DNA polymerase chain reaction chip," J. Micromechan. Microeng., vol. 12, pp. 813-823, 2002.
17. P. J. Obeid and T. K. Christopoulos, "Continuous-flow DNA and RNA amplification chip combined with laser-induced fluorescence detection," Analytica. Chimica. Acta, vol. 494, pp. 1-9, 2003.
18. P. C. Simpson, A. T. Woolley, and R. A. Mathies, "Microfabrication technology for the production of capillary array electrophoresis chips," Biomed. Microdev., vol. 1, pp. 7-26, 1998.
19. Q. Hao, "Analytical heat-transfer modeling of multilayered microdevices," J. Micromechan. Microeng., vol. 14, pp. 914-926, 2004.
20. D. Japikse and N. C. Baines, "Diffuser design technology," Concepts ETI, 1998.
21. C. D. Meinhart, S. T. Wereley, and J. G. Santiago, "PIV measurements of a microchannel flow," Exp. Fluids, vol. 27, pp. 414-419, 1999.
22. S. Devasenathipathy, J. G. Santiago, S. T. Wereley, C. D. Meinhart, and K. Takehara, "Particle imaging techniques for microfabricated fluidic systems," Exp. Fluids, vol. 34, pp. 504-514, 2003.
23. H. Li and M. G. Olsen, "MicroPIV measurements in square microchannels with hydraulic diameters from 200 mm to 640 mm," Int. J. Heat Fluid Flow. AUTHOR: PLEASE UPDATE, IF POSSIBLE - ED., to be published.
24. M. G. Olsen and R. J. Adrian, "Brownian motion and correlation in particle image velocimetry," Opt. Laser Technol., vol. 32, pp. 621-627, 2000.
25. C. J. Bourdon, M. G. Olsen, and A. D. Gorby, "Validation of analytical solution for depth of correlation in microscopic particle image velocimetry," Measure. Sci. Technol., vol. 15, pp. 318-327, 2004.
26. A. K. Prasad, R. J. Adrian, C. C. Landreth, and P. W. Offutt, "Effect of resolution on the speed and accuracy of particle image velocimetry interrogation," Exp. Fluids, vol. 13, pp. 105-16, 1992.
27. R. J. Adrian and C. S. Yao, "Pulsed laser technique application to liquid and gaseous flows and the scattering power of seed material," Appl. Opt., vol. 24, pp. 42-52, 1983.