Microfabrication Technology for the Production of Capillary Array Electrophoresis Chips

Biomedical Microdevices

Volumn 1, Issue 1, 1998, Pages 7-26

  Simpson, Peter C ^a   Woolley, Adam T ^a   Mathies, Richard A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Capillary array electrophoresis; Capillary electrophoresis chips; Confocal fluorescence detection; DNA Analysis; Glass micromachining; Multiplex sample injection

Indexed keywords

EID: 0005397454     PISSN: 13872176     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1009922004301     Document Type: Article

References (27)

1. D.J. Harrison, A. Manz, Z. Fan, H. Ludi, and H.M. Widmer, Anal. Chem. 64, 1926-1932 (1992).
2. S.C. Jacobson, R. Hergenroeder, L.B. Koutny, R.J. Warmack, and J.M. Ramsey, Anal. Chem. 66, 1107-1113 (1994).
3. D.J. Harrison, K. Fluri, K. Seiler, Z. Fan, C.S. Effenhauser, and A. Manz, Science 261, 895-897 (1993).
4. C.S. Effenhauser, A. Manz, H.M. Widmer, Anal. Chem. 65, 2637-2642 (1993).
5. S.C. Jacobson, R. Hergenroder, A.W. Moore., and J.M. Ramsey, Anal. Chem. 66, 4127-4132 (1994).
6. S.C. Jacobson, A.W. Moore, and J.M. Ramsey, Anal. Chem. 67, 2059-2063 (1995).
7. L.B. Koutny, D. Schmalzing, T.A. Taylor, and M. Fuchs, Anal. Chem. 68, 18-22 (1996).
8. A.T. Woolley and R.A. Mathies, Proc. Natl. Acad. Sci. 91, 11348-11352 (1994).
9. S.C. Jacobson and J.M. Ramsey, Anal. Chem. 68, 720-723 (1996).
10. C.S. Effenhauser, A. Paulus, A. Manz, and H.M. Widmer, Anal. Chem. 66, 2949-2953 (1994).
11. A.T. Woolley and R.A. Mathies, Anal. Chem. 67, 3676-3680 (1995).
12. M.A. Northrup, M.T. Ching, R.M. White, and R.T. Watson, Digest of Technical Papers: Transducers 1993 (IEEE, New York, NY, 1993), pp. 924-926.
13. M.A. Burns, C.H. Mastrangelo, T.S. Sammarco, F.P. Man, J.R. Webster, B.N. Johnson, B. Foerster, D. Jones, Y. Fields, A.R. Kaiser, and D.T. Burke, Proc. Natl. Acad. Sci. U.S.A. 93, 5556-5561 (1996).
14. A.T. Woolley, D. Hadley, P. Landre, A.J. deMello, R.A. Mathies, and M.A. Northrup, Anal. Chem. 68, 4081-4086 (1996).
15. Z. Liang, N. Chiem, G. Ocvirk, T. Tang, K. Fluri, and D.J. Harrison, Anal. Chem. 68, 1040-1046 (1996).
16. R.A. Mathies and X.C. Huang, Nature 359, 167-169 (1992).
17. A.T. Woolley, G.F. Sensabaugh, and R.A. Mathies, Anal. Chem. 69, 2181-2186 (1997).
18. K. Fluri, G. Fitzpatrick, N. Chiem, and D.J. Harrison, Anal. Chem. 68, 4285-4290 (1996).
19. Z.H. Fan and D.J. Harrison, Anal. Chem. 66, 177-184 (1994).
20. P.C. Simpson, Capillary Electrophoresis Chip Fabrication and Improvements in DNA Sequencing Resolution, M. Eng. Degree Project, Cornell University, 1996.
21. G.R. Cokelet, R. Soave, G. Pugh, and L. Rathbun, Microvascular Research 46, 394-400 (1993).
22. I. Kheterpal, J.R. Scherer, S.M. Clark, A. Radhakrishnan, J. Ju, C.L. Ginther, G.F. Sensabaugh, and R.A. Mathies, Electrophoresis 17, 1852-1859 (1996).
23. O. Trepte and A. Liljeborg, Opt. Eng. 33, 3774-3780 (1994).
24. We evaluated CCD detection of the CAE chips using a line focused laser beam. We found that rather high laser power (∼ 300 mW) was necessary to optimally excite fluorescence in the channels and that background fluorescence from the CAE chip reduced the signal-to-noise ratio. The DNA detection sensitivity with the CCD was therefore about 10 × worse than with the confocal detection system, primarily because of the higher background glass fluorescence due to poorer spatial filtering with the CCD system. The scanning confocal system was therefore preferred because of its better detection limits and lower laser power requirements.
25. R.L. Brumley, Jr. and L.M. Smith, Nucleic Acids Res. 19, 4121-4126 (1991).
26. S. Hjerten, J. Chromatogr. 347, 191-198 (1985).
27. Y. Xia, E. Kim, X.-M. Zhao, J.A. Rogers, M. Prentiss, and G.M. Whitesides, Science 273, 347-349 (1996).