X-ray structures of a hydrolytic antibody and of complexes elucidate catalytic pathway from substrate binding and transition state stabilization through water attack and product release

Proceedings of the National Academy of Sciences of the United States of America

Volumn 94, Issue 15, 1997, Pages 7857-7861

  Gigant, Benoît ^a   Charbonnier, Jean Baptiste ^a,d   Eshhar, Zelig ^b   Green, Bernard S ^c   Knossow, Marcel ^a  

^a LABORATOIRE D ENZYMOLOGIE ET BIOCHIMIE STRUCTURALES   (France)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^c HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^d Commsrt à l'Energie Atomique ^*  (France)

Author keywords

Catalysis; Catalytic antibody; Ester hydrolysis; Oxyanion hole; Phosphonate hapten

Indexed keywords

ANTIBODY; HAPTEN; PHOSPHONIC ACID DERIVATIVE;

ANTIGEN ANTIBODY COMPLEX; ARTICLE; BINDING SITE; CATALYSIS; CRYSTAL STRUCTURE; HYDROLYSIS; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN STRUCTURE; X RAY ANALYSIS;

ANTIBODIES, CATALYTIC; ANTIBODY SPECIFICITY; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; HYDROLYSIS; IMMUNOGLOBULIN FAB FRAGMENTS; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; WATER;

EID: 0030759620     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.94.15.7857     Document Type: Article

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