Effect of membrane perturbants on the activity and phase distribution of inositol phosphorylceramide synthase; development of a novel assay

Biochemistry

Volumn 43, Issue 26, 2004, Pages 8483-8493

  Aeed, Paul A ^a   Sperry, Andrea E ^a,c   Young, Casey L ^a,d   Nagiec, Marek M ^a   Elhammer, Åke P ^a,b  

^a PFIZER INC   (United States)

^b AureoGen Biosciences Inc   (United States)

^c ELI LILLY AND COMPANY   (United States)

^d Replidyne Corporation   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DETERGENTS; ENZYME INHIBITION; ENZYMES; PROTEINS; SOLUTIONS; SUBSTRATES; SYNTHESIS (CHEMICAL);

KINETIC PARAMETERS; MEMBRANE PERTURBANTS; MOLECULAR MASS; PHASE DISTRIBUTION;

CELL MEMBRANES;

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; 3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 2 HYDROXY 1 PROPANESULFONIC ACID; AMPHOLYTE; DETERGENT; INOSITOLPHOSPHORYLCERAMIDE SYNTHASE; MEMBRANE PROTEIN; PHOSPHOLIPID; SPHINGOSINE ACYLTRANSFERASE; TAURODEOXYCHOLIC ACID; TRITON X 100; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INACTIVATION; ENZYME PURIFICATION; ENZYME SUBSTRATE; EXTRACTION; GOLGI COMPLEX; LIPOGENESIS; PHASE TRANSITION; PRIORITY JOURNAL; SEDIMENT; SOLUBILIZATION;

4-CHLORO-7-NITROBENZOFURAZAN; BIOCHEMISTRY; BUTYRATES; CANDIDA ALBICANS; CENTRIFUGATION, DENSITY GRADIENT; CERAMIDES; CETOMACROGOL; CHOLIC ACIDS; CHROMATOGRAPHY; DETERGENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; GLUCOSIDES; HEXOSYLTRANSFERASES; KINETICS; LIPIDS; LYSOPHOSPHOLIPIDS; OCTOXYNOL; PHOSPHOLIPIDS; POLYETHYLENE GLYCOLS; POLYSORBATES; QUATERNARY AMMONIUM COMPOUNDS; TAURODEOXYCHOLIC ACID; TIME FACTORS;

CANDIDA; CANDIDA ALBICANS;

EID: 3142655272     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049141u     Document Type: Article

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