메뉴 건너뛰기




Volumn 43, Issue 26, 2004, Pages 8510-8516

Cysteine-scanning mutagenesis of the fifth external loop of serotonin transporter

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICALS REMOVAL (WATER TREATMENT); ENZYMES; REACTION KINETICS; SODIUM; SUBSTRATES; TRANSPORT PROPERTIES;

EID: 3142583008     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035971g     Document Type: Article
Times cited : (26)

References (38)
  • 1
    • 0001951796 scopus 로고    scopus 로고
    • Mechanisms of biogenic amine neurotransmitter transporters
    • (Reith, M. E. A., Ed.) 2nd ed. Humana Press, Totowa, NJ
    • Rudnick, G. (2002) Mechanisms of Biogenic Amine Neurotransmitter Transporters, in Neurotransmitter Transporters, Structure, Function, and Regulation (Reith, M. E. A., Ed.) 2nd ed., pp 25-52, Humana Press, Totowa, NJ.
    • (2002) Neurotransmitter Transporters, Structure, Function, and Regulation , pp. 25-52
    • Rudnick, G.1
  • 2
    • 0036431660 scopus 로고    scopus 로고
    • The Transporter Classification (TC) system
    • Busch, W., and Saier, M. H. (2002) The Transporter Classification (TC) system, Crit. Rev. Biochem. Mol. Biol. 37, 287-337.
    • (2002) Crit. Rev. Biochem. Mol. Biol. , vol.37 , pp. 287-337
    • Busch, W.1    Saier, M.H.2
  • 3
    • 0026419334 scopus 로고
    • Cloning and expression of a functional serotonin transporter from rat brain
    • Blakely, R., Berson, H., Fremeau, R., Caron, M., Peek, M., Prince, H., and Bradely, C. (1991) Cloning and Expression of a Functional Serotonin Transporter from Rat Brain, Nature 354, 66-70.
    • (1991) Nature , vol.354 , pp. 66-70
    • Blakely, R.1    Berson, H.2    Fremeau, R.3    Caron, M.4    Peek, M.5    Prince, H.6    Bradely, C.7
  • 4
    • 0026350637 scopus 로고
    • Cloning of a serotonin transporter affected by antidepressants
    • Hoffman, B. J., Mezey, E., and Brownstein, M. J. (1991) Cloning of a Serotonin Transporter Affected by Antidepressants, Science 254, 579-580.
    • (1991) Science , vol.254 , pp. 579-580
    • Hoffman, B.J.1    Mezey, E.2    Brownstein, M.J.3
  • 5
    • 0032524637 scopus 로고    scopus 로고
    • Determination of external loop topology in the serotonin transporter by site-directed chemical labeling
    • Chen, J. G., Liu-Chen, S., and Rudnick, G. (1998) Determination of External Loop Topology in the Serotonin Transporter By Site-Directed Chemical Labeling, J. Biol. Chem. 273, 12675-12681.
    • (1998) J. Biol. Chem. , vol.273 , pp. 12675-12681
    • Chen, J.G.1    Liu-Chen, S.2    Rudnick, G.3
  • 6
    • 0036812497 scopus 로고    scopus 로고
    • Accessibility and conformational coupling in serotonin transporter predicted internal domains
    • Androutsellis-Theotokis, A., and Rudnick, G. (2002) Accessibility and conformational coupling in serotonin transporter predicted internal domains, J. Neurosci. 22, 8370-8378.
    • (2002) J. Neurosci. , vol.22 , pp. 8370-8378
    • Androutsellis-Theotokis, A.1    Rudnick, G.2
  • 7
    • 0020475449 scopus 로고
    • A simple method for displaying the hydropathic character of a protein
    • Kyte, J., and Doolittle, R. F. (1982) A simple method for displaying the hydropathic character of a protein, J. Mol. Biol. 157, 105-132.
    • (1982) J. Mol. Biol. , vol.157 , pp. 105-132
    • Kyte, J.1    Doolittle, R.F.2
  • 8
    • 0022510143 scopus 로고
    • Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins
    • Engelman, D. M., Steitz, T. A., and Goldman, A. (1986) Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins, Annu. Rev. Biophys. Biophys. Chem. 15, 321-353.
    • (1986) Annu. Rev. Biophys. Biophys. Chem. , vol.15 , pp. 321-353
    • Engelman, D.M.1    Steitz, T.A.2    Goldman, A.3
  • 9
    • 0030692041 scopus 로고    scopus 로고
    • The third transmembrane domain of the serotonin transporter contains residues associated with substrate and cocaine binding
    • Chen, J. G., Sachpatzidis, A., and Rudnick, G. (1997) The Third Transmembrane Domain of the Serotonin Transporter Contains Residues Associated With Substrate and Cocaine Binding, J. Biol. Chem. 272, 28321-28327.
    • (1997) J. Biol. Chem. , vol.272 , pp. 28321-28327
    • Chen, J.G.1    Sachpatzidis, A.2    Rudnick, G.3
  • 10
    • 0033981373 scopus 로고    scopus 로고
    • Permeation and gating residues in serotonin transporter
    • Chen, J. G., and Rudnick, G. (2000) Permeation and gating residues in serotonin transporter, Proc. Natl. Acad. Sci. U.S.A. 97, 1044-1049.
    • (2000) Proc. Natl. Acad. Sci. U.S.A , vol.97 , pp. 1044-1049
    • Chen, J.G.1    Rudnick, G.2
  • 11
    • 0032584587 scopus 로고    scopus 로고
    • High affinity recognition of serotonin transporter antagonists defined by species-scanning mutagenesis-an aromatic residue in transmembrane domain I dictates species-selective recognition of citalopram and mazindol
    • Barker, E. L., Perlman, M. A., Adkins, E. M., Houlihan, W. J., Pristupa, Z. B., Niznik, H. B., and Blakely, R. D. (1998) High Affinity Recognition of Serotonin Transporter Antagonists Defined By Species-Scanning Mutagenesis-An Aromatic Residue in Transmembrane Domain I Dictates Species-Selective Recognition of Citalopram and Mazindol, J. Biol. Chem. 273, 19459-19468.
    • (1998) J. Biol. Chem. , vol.273 , pp. 19459-19468
    • Barker, E.L.1    Perlman, M.A.2    Adkins, E.M.3    Houlihan, W.J.4    Pristupa, Z.B.5    Niznik, H.B.6    Blakely, R.D.7
  • 12
    • 0033564765 scopus 로고    scopus 로고
    • Transmembrane domain I contributes to the permeation pathway for serotonin and ions in the serotonin transporter
    • Barker, E. L., Moore, K. R., Rakhshan, F., and Blakely, R. D. (1999) Transmembrane domain I contributes to the permeation pathway for serotonin and ions in the serotonin transporter, J. Neurosci. 19, 4705-4717.
    • (1999) J. Neurosci. , vol.19 , pp. 4705-4717
    • Barker, E.L.1    Moore, K.R.2    Rakhshan, F.3    Blakely, R.D.4
  • 13
    • 0141844649 scopus 로고    scopus 로고
    • Serotonin and cocaine-sensitive inactivation of human serotonin transporters by methanethiosulfonates targeted to transmembrane domain 1
    • Henry, L. K., Adkins, E. M., Han, Q., and Blakely, R. D. (2003) Serotonin and cocaine-sensitive inactivation of human serotonin transporters by methanethiosulfonates targeted to transmembrane domain 1, J. Biol. Chem. 278, 37052-37063.
    • (2003) J. Biol. Chem. , vol.278 , pp. 37052-37063
    • Henry, L.K.1    Adkins, E.M.2    Han, Q.3    Blakely, R.D.4
  • 14
    • 0031017637 scopus 로고    scopus 로고
    • An extracellular loop region of the serotonin transporter may be involved in the translocation mechanism
    • Stephan, M. M., Chen, M., A., Penado, K. M., and Rudnick, G. (1997) An extracellular loop region of the serotonin transporter may be involved in the translocation mechanism, Biochemistry 36, 1322-1328.
    • (1997) Biochemistry , vol.36 , pp. 1322-1328
    • Stephan, M.M.1    Chen, M.A.2    Penado, K.M.3    Rudnick, G.4
  • 15
    • 0033579506 scopus 로고    scopus 로고
    • The role of external loop regions in serotonin transport-Loop scanning mutagenesis of the serotonin transporter external domain
    • Smicun, Y., Campbell, S. D., Chen, M. A., Gu, H., and Rudnick, G. (1999) The role of external loop regions in serotonin transport-Loop scanning mutagenesis of the serotonin transporter external domain, J. Biol. Chem. 274, 36058-36064.
    • (1999) J. Biol. Chem. , vol.274 , pp. 36058-36064
    • Smicun, Y.1    Campbell, S.D.2    Chen, M.A.3    Gu, H.4    Rudnick, G.5
  • 16
    • 0032190185 scopus 로고    scopus 로고
    • Amino acid residues that control pH modulation of transport-associated current in mammalian serotonin transporters
    • Cao, Y., Li, M., Mager, S., and Lester, H. A. (1998) Amino acid residues that control pH modulation of transport-associated current in mammalian serotonin transporters, J. Neurosci. 18, 7739-7749.
    • (1998) J. Neurosci. , vol.18 , pp. 7739-7749
    • Cao, Y.1    Li, M.2    Mager, S.3    Lester, H.A.4
  • 17
    • 0035886678 scopus 로고    scopus 로고
    • Mutation K448E in the external loop 5 of rat GABA transporter rGAT1 induces pH sensitivity and alters substrate interactions
    • Forlani, G., Bossi, E., Ghirardelli, R., Giovannardi, S., Binda, F., Bonadiman, L., Ielmini, L., and Peres, A. (2001) Mutation K448E in the external loop 5 of rat GABA transporter rGAT1 induces pH sensitivity and alters substrate interactions, J. Physiol. 536, 479-494.
    • (2001) J. Physiol. , vol.536 , pp. 479-494
    • Forlani, G.1    Bossi, E.2    Ghirardelli, R.3    Giovannardi, S.4    Binda, F.5    Bonadiman, L.6    Ielmini, L.7    Peres, A.8
  • 18
    • 0027960964 scopus 로고
    • --dependent serotonin transporter expressed using recombinant baculovirus in insect cells
    • --dependent serotonin transporter expressed using recombinant baculovirus in insect cells, J. Biol. Chem. 269, 26303-26310.
    • (1994) J. Biol. Chem. , vol.269 , pp. 26303-26310
    • Tate, C.1    Blakely, R.2
  • 19
    • 0031033676 scopus 로고    scopus 로고
    • External cysteine residues in the serotonin transporter
    • Chen, J. G., Liu-Chen, S., and Rudnick, G. (1997) External Cysteine Residues in the Serotonin Transporter, Biochemistry 36, 1479-1486.
    • (1997) Biochemistry , vol.36 , pp. 1479-1486
    • Chen, J.G.1    Liu-Chen, S.2    Rudnick, G.3
  • 20
    • 0025890297 scopus 로고
    • Vaccinia-T7 RNA polymerase expression system: Evaluation for the expression cloning of plasma membrane transporters
    • Blakely, R. D., Clark, J. A., Rudnick, G., and Amara, S. G. (1991) Vaccinia-T7 RNA Polymerase Expression System: Evaluation for the Expression Cloning of Plasma Membrane Transporters, Anal. Biochem. 194, 302-308.
    • (1991) Anal. Biochem. , vol.194 , pp. 302-308
    • Blakely, R.D.1    Clark, J.A.2    Rudnick, G.3    Amara, S.G.4
  • 21
    • 0009482260 scopus 로고
    • Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
    • Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications, Proc. Natl. Acad. Sci. U.S.A. 76, 4350-4354.
    • (1979) Proc. Natl. Acad. Sci. U.S.A. , vol.76 , pp. 4350-4354
    • Towbin, H.1    Staehelin, T.2    Gordon, J.3
  • 22
    • 0028829467 scopus 로고
    • Short external loops as potential substrate binding site of γ-aminobutyric acid transporters
    • Tamura, S., Nelson, H., Tamura, A., and Nelson, N. (1995) Short External Loops As Potential Substrate Binding Site Of γ-Aminobutyric Acid Transporters, J. Biol. Chem. 270, 28712-28715.
    • (1995) J. Biol. Chem. , vol.270 , pp. 28712-28715
    • Tamura, S.1    Nelson, H.2    Tamura, A.3    Nelson, N.4
  • 23
    • 0033579506 scopus 로고    scopus 로고
    • The role of external loop regions in serotonin transport. Loop scanning mutagenesis of the serotonin transporter external domain
    • Smicun, Y., Campbell, S. D., Chen, M. A., Gu, H., and Rudnick, G. (1999) The role of external loop regions in serotonin transport. Loop scanning mutagenesis of the serotonin transporter external domain, J. Biol. Chem. 274, 36058-36064.
    • (1999) J. Biol. Chem. , vol.274 , pp. 36058-36064
    • Smicun, Y.1    Campbell, S.D.2    Chen, M.A.3    Gu, H.4    Rudnick, G.5
  • 24
    • 0018110116 scopus 로고
    • Prediction of the secondary structure of proteins from their amino acid sequence
    • Chou, P. Y., and Fasman, G. D. (1978) Prediction of the secondary structure of proteins from their amino acid sequence, Adv. Enzymol. Relat. Areas Mol. Biol. 47, 45-148.
    • (1978) Adv. Enzymol. Relat. Areas Mol. Biol. , vol.47 , pp. 45-148
    • Chou, P.Y.1    Fasman, G.D.2
  • 25
    • 0021028736 scopus 로고
    • Assessment of secondary-structure prediction of proteins. Comparison of computerized Chou-Fasman method with others
    • Nishikawa, K. (1983) Assessment of secondary-structure prediction of proteins. Comparison of computerized Chou-Fasman method with others, Biochim. Biophys. Acta 748, 285-299.
    • (1983) Biochim. Biophys. Acta , vol.748 , pp. 285-299
    • Nishikawa, K.1
  • 26
    • 0017873321 scopus 로고
    • Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins
    • Garnier, J., Osguthorpe, D. J., and Robson, B. (1978) Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins, J. Mol. Biol. 120, 97-120.
    • (1978) J. Mol. Biol. , vol.120 , pp. 97-120
    • Garnier, J.1    Osguthorpe, D.J.2    Robson, B.3
  • 27
    • 0141582543 scopus 로고    scopus 로고
    • Chemical modification strategies for structure-function studies
    • (Quick, M. W., Ed.), Wiley-Liss, Inc., Hoboken, NJ
    • Rudnick, G. (2002) Chemical Modification Strategies for Structure-Function Studies, in Transmembrane Transporters (Quick, M. W., Ed.) pp 125-141, Wiley-Liss, Inc., Hoboken, NJ.
    • (2002) Transmembrane Transporters , pp. 125-141
    • Rudnick, G.1
  • 28
    • 0028355745 scopus 로고
    • Electrostatic potential of the acetylcholine binding sites in the nicotinic receptor probed by reactions of binding-site cysteines with charged methanethiosulfonates
    • Stauffer, D. A., and Karlin, A. (1994) Electrostatic potential of the acetylcholine binding sites in the nicotinic receptor probed by reactions of binding-site cysteines with charged methanethiosulfonates, Biochemistry 33, 6840-6849.
    • (1994) Biochemistry , vol.33 , pp. 6840-6849
    • Stauffer, D.A.1    Karlin, A.2
  • 29
    • 0035903134 scopus 로고    scopus 로고
    • A lithium-induced conformational change in serotonin transporter alters cocaine binding, ion conductance, and reactivity of cys-109
    • Ni, Y. G., Chen, J. G., Androutsellis-Theotokis, A., Huang, C. J., Moczydlowski, E., and Rudnick, G. (2001) A lithium-induced conformational change in serotonin transporter alters cocaine binding, ion conductance, and reactivity of cys-109, J. Biol. Chem. 276, 30942-30947.
    • (2001) J. Biol. Chem. , vol.276 , pp. 30942-30947
    • Ni, Y.G.1    Chen, J.G.2    Androutsellis-Theotokis, A.3    Huang, C.J.4    Moczydlowski, E.5    Rudnick, G.6
  • 30
    • 0035824585 scopus 로고    scopus 로고
    • A conformationally sensitive residue on the cytoplasmic surface of serotonin transporter
    • Androutsellis-Theotokis, A., Ghassemi, F., and Rudnick, G. (2001) A Conformationally Sensitive Residue on the Cytoplasmic Surface of Serotonin Transporter, J. Biol. Chem. 276, 45933-45938.
    • (2001) J. Biol. Chem. , vol.276 , pp. 45933-45938
    • Androutsellis-Theotokis, A.1    Ghassemi, F.2    Rudnick, G.3
  • 31
    • 0035122827 scopus 로고    scopus 로고
    • Interactions of tryptamine derivatives with serotonin transporter species variants implicate transmembrane domain I in substrate recognition
    • Adkins, E. M., Barker, E. L., and Blakely, R. D. (2001) Interactions of Tryptamine Derivatives with Serotonin Transporter Species Variants Implicate Transmembrane Domain I in Substrate Recognition, Mol. Pharmacol. 59, 514-523.
    • (2001) Mol. Pharmacol. , vol.59 , pp. 514-523
    • Adkins, E.M.1    Barker, E.L.2    Blakely, R.D.3
  • 32
    • 0034105641 scopus 로고    scopus 로고
    • Mutational analysis of basic residues in the rat vesicular acetylcholine transporter. Identification of a transmembrane ion pair and evidence that histidine is not involved in proton translocation
    • Kim, M. H., Lu, M., Kelly, M., and Hersh, L. B. (2000) Mutational Analysis of Basic Residues in the Rat Vesicular Acetylcholine Transporter. Identification of a transmembrane ion pair and evidence that histidine is not involved in proton translocation, J. Biol. Chem. 275, 6175-6180.
    • (2000) J. Biol. Chem. , vol.275 , pp. 6175-6180
    • Kim, M.H.1    Lu, M.2    Kelly, M.3    Hersh, L.B.4
  • 33
    • 0026087282 scopus 로고
    • The interaction between aspartic acid 237 and lysine 358 in the lactose carrier of Escherichia coli
    • King, S. C., Hansen, C. L., and Wilson, T. H. (1991) The interaction between aspartic acid 237 and lysine 358 in the lactose carrier of Escherichia coli, Biochim. Biophys. Acta 1062, 177-186.
    • (1991) Biochim. Biophys. Acta , vol.1062 , pp. 177-186
    • King, S.C.1    Hansen, C.L.2    Wilson, T.H.3
  • 34
    • 0027382884 scopus 로고
    • Properties of Interacting Aspartic Acid and Lysine Residues in the Lactose Permease of Escherichia coli
    • Sahin-Toth, M., and Kaback, H. R. (1993) Properties of Interacting Aspartic Acid and Lysine Residues in the Lactose Permease of Escherichia coli, Biochemistry 32, 10027-10035.
    • (1993) Biochemistry , vol.32 , pp. 10027-10035
    • Sahin-Toth, M.1    Kaback, H.R.2
  • 35
    • 0031054527 scopus 로고    scopus 로고
    • Charged residues in transmembrane domains II and XI of a vesicular monoamine transporter form a charge pair that promotes high affinity substrate recognition
    • Merickel, A., Kaback, H. R., and Edwards, R. H. (1997) Charged residues in transmembrane domains II and XI of a vesicular monoamine transporter form a charge pair that promotes high affinity substrate recognition, J. Biol. Chem. 272 (9), 5403-5408.
    • (1997) J. Biol. Chem. , vol.272 , Issue.9 , pp. 5403-5408
    • Merickel, A.1    Kaback, H.R.2    Edwards, R.H.3
  • 36
    • 2642549055 scopus 로고    scopus 로고
    • Conformation and accessibility in external loop 4 of serotonin transporter
    • Mitchell, S. M., and Stephan, M. M. (2004) Conformation and Accessibility in External loop 4 of Serotonin Transporter, J. Biol. Chem. 279, 24089-24099.
    • (2004) J. Biol. Chem. , vol.279 , pp. 24089-24099
    • Mitchell, S.M.1    Stephan, M.M.2
  • 37
    • 2542507235 scopus 로고    scopus 로고
    • Investigation of transmembrane domain 2 of rat serotonin transporter
    • Sato, Y., Androutsellis-Theotokis, A., and Rudnick, G. (2004) Investigation of Transmembrane Domain 2 of Rat Serotonin Transporter, J. Biol. Chem. 279, 22926-22933.
    • (2004) J. Biol. Chem. , vol.279 , pp. 22926-22933
    • Sato, Y.1    Androutsellis-Theotokis, A.2    Rudnick, G.3
  • 38
    • 0027968068 scopus 로고
    • Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalites, and weight matrix choice
    • Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalites, and weight matrix choice, Nucleic Acids Res. 22, 4673-4680.
    • (1994) Nucleic Acids Res. , vol.22 , pp. 4673-4680
    • Thompson, J.D.1    Higgins, D.G.2    Gibson, T.J.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.