Accessibility and conformational coupling in serotonin transporter predicted internal domains

Journal of Neuroscience

Volumn 22, Issue 19, 2002, Pages 8370-8378

  Androutsellis Theotokis, Andreas ^a   Rudnick, Gary ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Binding; Conformation; Cytoplasmic; Serotonin; Topology; Transporter

Indexed keywords

4 AMINOBUTYRIC ACID CARRIER; AMINO ACID TRANSPORTER; BIOTIN; CARRIER PROTEIN; CYSTEINE; DIGITONIN; GLYCINE; GLYCINE TRANSPORTER; METHANETHIOSULFONATE; SEROTONIN TRANSPORTER; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; COMPUTER PREDICTION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CYTOPLASM; HUMAN; HUMAN CELL; LIGAND BINDING; MEMBRANE PERMEABILITY; NEUROTRANSMITTER RELEASE; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN MODIFICATION; REACTION ANALYSIS; SEROTONIN RELEASE;

EID: 0036812497     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.22-19-08370.2002     Document Type: Article

References (30)

1. Amara S, Kuhar M (1993) Neurotransmitter transporters: Recent progress. Annu Rev Neurosci 16:73-93.
2. Androutsellis-Theotokis A, Ghassemi F, Rudnick G (2001) A Conformationally sensitive residue on the cytoplasmic surface of serotonin transporter. J Biol Chem 276:45933-45938.
3. Beck LA, Hosick TJ, Sinensky M (1988) Incorporation of a product of mevalonic acid metabolism into proteins of Chinese hamster ovary cell nuclei. J Cell Biol 107:1307-1316.
4. ++Cl-)-coupled gamma-aminobutyric acid transporter from rat Brain. J Biol Chem 272:1203-1210.
5. Blakely R, Berson H, Fremeau R, Caron M, Peek M, Prince H, Bradely C (1991a) Cloning and expression of a functional serotonin transporter from rat brain. Nature 354:66-70.
6. Blakely RD, Clark JA, Rudnick G, Amara SG (1991b) Vaccinia-T7 RNA polymerase expression system: Evaluation for the expression cloning of plasma membrane transporters. Anal Biochem 194:302-308.
7. Bruss M, Hammermann R, Brimijoin S, Bonisch H (1995) Antipeptide antibodies confirm the topology of the human norepinephrine transporter. J Biol Chem 270:9197-9201.
8. Chen JG, Rudnick G (2000) Permeation and gating residues in serotonin transporter. Proc Natl Acad Sci USA 97:1044-1049.
9. Chen JG, Liu-Chen S, Rudnick G (1997) External cysteine residues in the serotonin transporter. Biochemistry 36:1479-1486.
10. Chen JG, Liu-Chen S, Rudnick G (1998) Determination of external loop topology in the serotonin transporter by site-directed chemical labeling. J Biol Chem 273:12675-12681.
11. Clark JA (1997) Analysis of the transmembrane topology and membrane assembly of the GAT-1 gamma-aminobutyric acid transporter. J Biol Chem 272:14695-14704.
12. Ferrer JV, Javitch JA (1998) Cocaine alters the accessibility of endogenous cysteines in putative extracellular and intracellular loops of the human dopamine transporter. Proc Natl Acad Sci USA 95:9238-9243.
13. Guastella J, Nelson N, Nelson H, Czyzyk L, Keynan S, Miedel M, Davidson N, Lester H, Kanner BI (1990) Cloning and expression of a rat brain GABA transporter. Science 249:1303-1306.
14. Harlow E, Lane DP (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
15. Hersch SM, Yi H, Heilman CJ, Edwards RH, Levey AI (1997) Subcellular localization and molecular topology of the dopamine transporter in the striatum and substantia nigra. J Comp Neurol 388:211-227.
16. Hoffman BJ, Mezey E, Brownstein MJ (1991) Cloning of a serotonin transporter affected by antidepressants. Science 254:579-580.
17. Holmgren M, Liu Y, Xu Y, Yellen G (1996) On the use of thiol-modifying agents to determine channel topology. Neuropharmacology 35:797-804.
18. Kilic F, Rudnick G (2000) Oligomerization of serotonin transporter and its functional consequences. Proc Natl Acad Sci USA 97:3106-3111.
19. Liu Q-R, Lopez-corcuera B, Mandiyan S, Nelson H, Nelson N (1993) Cloning and expression of a spinal cord-specific and brain-Specific glycine transporter with novel structural features. J Biol Chem 268:22802-22808.
20. - neurotransmitter transporters. J Neurochem 71:1785-1803.
21. Ni YG, Chen JG, Androutsellis-Theotokis A, Huang CJ, Moczydlowski E, Rudnick G (2001) A lithium-induced conformational change in serotonin transporter alters cocaine binding, ion conductance, and reactivity of Cys-109. J Biol Chem 276:30942-30947.
22. Olivares L, Aragon C, Gimenez C, Zafra F (1997) Analysis of the transmembrane topology of the glycine transporter Glyt1. J Biol Chem 272:1211-1217.
23. Pacholczyk T, Blakely R, Amara S (1991) Expression cloning of a cocaine- and antidepressant-sensitive human noradrenaline transporter. Nature 350:350-354.
24. Ramamoorthy S, Bauman A, Moore K, Han H, Yang-Feng T, Chang A, Ganapathy V, Blakely R (1993) Antidepressant and cocaine sensitive human serotonin transporter: Molecular cloning, expression, and chromosomal localization. Proc Natl Acad Sci USA 90:2542-2546.
25. Rudnick G (1997) Mechanisms of biogenic amine neurotransmitter transporters. In: Neurotransmitter Transporters: Structure, Function, and Regulation (Reith M, ed), pp 73-100. Totowa, NJ: Humana.
26. Rudnick G (1998) Bioenergetics of neurotransmitter transport. J Bioenerg Biomembr 30:173-185.
27. Rudnick G, Clark J (1993) From synapse to vesicle: The reuptake and storage of biogenic amine neurotransmitters. Biochim Biophys Acta 1144:249-263.
28. Shimada S, Kitayama S, Lin C, Patel A, Nanthakumar E, Gregor P, Kuhar M, Uhl G (1991) Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA. Science 254:576-578.
29. +- and Cl -dependent serotonin transporter expressed using recombinant baculovirus in insect cells. J Biol Chem 269:26303-26310.
30. Uhl G, Johnson P (1994) Neurotransmitter transporters: Three important gene families for neuronal function. J Exp Biol 196:229-236.