Tissue preparation for gene expression profiling of colorectal carinoma: Three alternatives to laser microdissection with preamplification

Journal of Laboratory and Clinical Medicine

Volumn 143, Issue 6, 2004, Pages 344-351

  Croner, Roland S ^a   Guenther, Klaus ^b   Foertsch, Thomas ^b   Siebenhaar, Renate ^b   Brueckl, Wolfgang M ^b   Stremmel, Christian ^b   Hlubek, Falk ^b   Hohenberger, Werner ^b   Reingruber, Bertram ^b  

^a UNIVERSITY HOSPITAL ERLANGEN   (Germany)

^b NONE

Author keywords

CMD; cryotomy after macroscopic manual dissection; DB; GAPDH; glyceraldehyde 3 phosphatase; H E; hematoxylin and eosin; laser capture microdissection; LCM; MAMD; tumor cell isolation with the use of Ber EP4 antibodies and Dynabeads

Indexed keywords

ANTIBODY; APC PROTEIN; BETA ACTIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE (NADP); K RAS PROTEIN; PROTEIN P53; RNA; SMAD PROTEIN; TRANSFORMING GROWTH FACTOR BETA;

ARTICLE; CANCER TISSUE; CELL ISOLATION; COLORECTAL CARCINOMA; CONTROLLED STUDY; CORRELATION ANALYSIS; DIAGNOSTIC PROCEDURE; DNA MICROARRAY; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; LASER MICRODISSECTION; SIGMOID; SIGMOID CARCINOMA;

ADENOCARCINOMA; CELL SEPARATION; COLONIC NEOPLASMS; COLORECTAL NEOPLASMS; DISSECTION; FROZEN SECTIONS; GENE EXPRESSION PROFILING; HISTOLOGICAL TECHNIQUES; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RNA, NEOPLASM;

EID: 3042788686     PISSN: 00222143     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.lab.2004.03.003     Document Type: Article

References (19)

1. Hedenfalk I., Duggan D., Chen Y., Radmacher M., Bittner R., Simon P., et al. Gene-expression profiles in hereditary breast cancer. N Engl J Med. 344:2001;539-548
2. Lau W.Y., Lai P.B., Leung M.F., Leung B.C., Wong N., Chen G., et al. Differential gene expression of hepatocellular carcinoma using cDNA microarray analysis. Oncol Res. 12:2000;59-69
3. Leethanakul C., Patel V., Gillespie J., Shillitoe E., Kellman R.M., Ensley J.F., et al. Gene expression profiles in squamous cell carconomas of the oral cavity use of laser capture microdissection for the construction and analysis of stage-specific cDNA libraries. Oral Oncol. 36:2000;474-483
4. Notterman D.A., Alon U., Sierk A.J., Levine A.J. Transcriptional gene expression profiles of colorectal adenoma, adenocarcinoma, and normal tissue examined by oligonucleotide arrays. Cancer Res. 61:2001;3124-3130
5. Hippo Y., Taniguchi H., Tsutsumi S., Machida N., Chong J.M., Fukayama T., et al. Global gene expression analysis of gastric cancer by oligonucleotide microarrays. Cancer Res. 62:2002;233-240
6. Stremmel C., Wein A., Hohenberger W., Reingruber B. DNA microarrays a new diagnostic tool and its implications in colorectal cancer. Int J Colorectal Dis. 17:2002;131-136
7. DeRisi J.L., Penland L., Brown P.O., Bittner M.L., Meltzer P.S., Ray M., et al. Use of a cDNA microarray to analyse gene expression patterns in human cancer. Nat Genet. 14:1996;457-460
8. Takemasa I., Higuchi H., Yamamoto H., Sekimoto M., Tomita N., Nakamori S., et al. construction of preferential cDNA microarray specialized for human colorectal carcinoma molecular sketch of colorectal cancer. Biochem Biophys Res Commun. 285:2001;1244-1249
9. Huang J., Qi R., Quackenbush J., Dauway E., Lazaridis E., Yeatman T., Graveel C.R., et al. Effects of ischemia on gene expression. Expression profiling and identification of novel genes in hepatocellular carcinomas. Tissue microarrays for gene amplification surverys in many different tumor types. J Surg Res. 99:2001;222-227
10. Emmert-Buck M.R., Bonner R.F., Smith P.D., Duaqui R.F., Zhuang Z., Goldstein S.R., et al. Laser capture microdissection. Science. 274:1996;998-1001
11. Imamichi Y., Lahr G., Wedlich D. Laser-mediated microdissection of paraffin sections from Xenopus embryos allows detection of tissue-specific expressed mRNAs. Dev Genes Evol. 211:2001;361-366
12. Rubin M.A. Use of laser capture microdissection, cDNA microarrays, and tissue microarrays in advancing our understanding of prostate cancer. J Pathol. 195:2001;80-86
13. Reymond M.A., Sanchez J.C., Hughes G.J., Gunther K., Riese J., Tortola S., et al. Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis. Electrophoresis. 18:1997;2842-2848
14. Nachamkin I., Panaro N.J., Li M., Ung H., Yuen P.K., Kricka L.J., et al. Agilent 2100 bioanalyzer for restriction fragment length polymorphism analysis of the Campylobacter jejuni flagellin gene. J Clin Microbiol. 39:2001;754-757
15. Hsiao L.L., Dangond F., Yoshida T., Hong R., Jensen R.V., Misra J., et al. A compendium of gene expression in normal human tissues. Physiol Genom. 7:2001;97-104
16. Kurian K.M., Watson C.J., Wyllie A.H. DNA chip technology. J Pathol. 187:1999;267-271
17. Arends J.W. Molecular interactions in the Vogelstein model of colorectal carcinoma. J Pathol. 190:2000;412-416
18. Puskas L.G., Zvara A., Hackler L. Jr, Van Hummelen P. RNA amplification results in reproducible microarray data with slight ratio bias. Biotechniques. 32:2002;1330-1334. 1336, 1338, 1340
19. Latza U., Niedobitek G., Schwarting R., Nekarda H., Stein H. Ber-EP4 new monoclonal antibody which distinguishes epithelia from mesothelia. J Clin Pathol. 43:1990;213-219