Saturation mutagenesis of toluene ortho-monooxygenase of Burkholderia cepacia G4 for enhanced 1-naphthol synthesis and chloroform degradation

Applied and Environmental Microbiology

Volumn 70, Issue 6, 2004, Pages 3246-3252

  Rui, Lingyun ^a   Kwon, Young Man ^a   Fishman, Ayelet ^a   Reardon, Kenneth F ^b   Wood, Thomas K ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

^b Engineering Research Center and Hydraulics Laboratory   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENETIC ENGINEERING; METABOLISM; OXIDATION; STATISTICAL METHODS;

CELL EXPRESSION; OXIDATION ACTIVITY;

MICROBIOLOGY;

1 NAPHTHOL; CHLORIDE; CHLOROFORM; NAPHTHALENE; ORTHO CRESOL; PARA CRESOL; TOLUENE ORTHO MONOOXYGENASE; TRICHLOROETHYLENE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

MICROBIOLOGY;

ALPHA CHAIN; ARTICLE; BACTERIUM COLONY; BIODEGRADATION; BURKHOLDERIA CEPACIA; CODON; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GAS CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; MUTAGENESIS; NONHUMAN; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BINDING SITES; BIODEGRADATION, ENVIRONMENTAL; BURKHOLDERIA CEPACIA; CHLOROFORM; CHROMATOGRAPHY, GAS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; CODON; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE LIBRARY; MIXED FUNCTION OXYGENASES; MODELS, MOLECULAR; MUTAGENESIS; NAPHTHOLS; OXIDATION-REDUCTION;

BACTERIA (MICROORGANISMS); BURKHOLDERIA; BURKHOLDERIA CEPACIA; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 2942565707     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.6.3246-3252.2004     Document Type: Article

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