Expression, purification, and characterization of cysteine-free mouse P-glycoprotein

Archives of Biochemistry and Biophysics

Volumn 445, Issue 1, 2006, Pages 124-128

  Tombline, Gregory ^a   Urbatsch, Ina L ^a   Virk, Navneet ^a   Muharemagic, Alma ^a   White, Lori Bartholomew ^a   Senior, Alan E ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

Active site tyrosine; ATPase mechanism; Cys free P glycoprotein; Multidrug resistance; P glycoprotein; Single Cys mutants

Indexed keywords

ABC TRANSPORTER; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATASE (MAGNESIUM); ALANINE; CYSTEINE; GLYCOPROTEIN P; MULTIDRUG RESISTANCE PROTEIN 1; MULTIDRUG RESISTANCE PROTEIN 3; TYROSINE; VANADIC ACID; VERAPAMIL;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CATALYSIS; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME SUBSTRATE; MOUSE; MUTAGENESIS; MUTANT; NONHUMAN; PHOTOAFFINITY LABELING; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; WILD TYPE; X RAY;

ADENOSINE TRIPHOSPHATE; AMINO ACID SUBSTITUTION; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; AZIDES; CATALYTIC DOMAIN; CYSTEINE; HUMANS; MICE; MUTATION; P-GLYCOPROTEIN; P-GLYCOPROTEINS; PHOTOAFFINITY LABELS; PICHIA;

PICHIA PASTORIS;

EID: 29244439332     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2005.11.004     Document Type: Article

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