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Volumn 33, Issue 6, 2005, Pages 1-6

An ultrasensitive site-specific DNA recombination assay based on dual-color fluorescence cross-correlation spectroscopy

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RECOMBINASE; DNA POLYMERASE;

EID: 28644447779     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gni060     Document Type: Article
Times cited : (17)

References (32)
  • 1
    • 0041375463 scopus 로고    scopus 로고
    • New insight into site-specific recombination from Flp recombinase-DNA structures
    • Chen,Y. and Rice,P.A. (2003) New insight into site-specific recombination from Flp recombinase-DNA structures. Annu. Rev. Biophys. Biomol. Str., 32, 135-159.
    • (2003) Annu. Rev. Biophys. Biomol. Str. , vol.32 , pp. 135-159
    • Chen, Y.1    Rice, P.A.2
  • 2
    • 0034622937 scopus 로고    scopus 로고
    • Chimeras of the flp and cre recombinases: Tests of the mode of cleavage by flp and cre
    • Shaikh,A.C. and Sadowski,P.D. (2000) Chimeras of the flp and cre recombinases: Tests of the mode of cleavage by flp and cre. J. Mol. Biol., 302, 27-48.
    • (2000) J. Mol. Biol. , vol.302 , pp. 27-48
    • Shaikh, A.C.1    Sadowski, P.D.2
  • 3
    • 0033637213 scopus 로고    scopus 로고
    • Crystal structure of a flp recombinase-holliday junction complex: Assembly of an active oligomer by helix swapping
    • Chen,Y., Narendra,U., Iype,L.E., Cox,M.M. and Rice,P.A. (2000) Crystal structure of a flp recombinase-holliday junction complex: Assembly of an active oligomer by helix swapping. Mol. Cell, 6, 885-897.
    • (2000) Mol. Cell , vol.6 , pp. 885-897
    • Chen, Y.1    Narendra, U.2    Iype, L.E.3    Cox, M.M.4    Rice, P.A.5
  • 4
    • 0028843052 scopus 로고
    • The Flp recombinase of the 2-microns plasmid of Saccharomycescerevisiae
    • Sadowski,P.D. (1995) The Flp recombinase of the 2-microns plasmid of Saccharomycescerevisiae. Prog. Nucleic Acid Res. Mol. Biol., 51, 53-91.
    • (1995) Prog. Nucleic Acid Res. Mol. Biol. , vol.51 , pp. 53-91
    • Sadowski, P.D.1
  • 5
    • 0035491607 scopus 로고    scopus 로고
    • Conditional control of gene expression in the mouse
    • Lewandoski,M. (2001) Conditional control of gene expression in the mouse. Nature Rev. Genet., 2, 743-755.
    • (2001) Nature Rev. Genet. , vol.2 , pp. 743-755
    • Lewandoski, M.1
  • 6
    • 0036363357 scopus 로고    scopus 로고
    • Application of FLP/FRT site-specific DNA recombination system inplants
    • Luo,H. and Kausch,A.P. (2002) Application of FLP/FRT site-specific DNA recombination system inplants. Genet. Eng. (New York), 24, 1-16.
    • (2002) Genet. Eng. (New York) , vol.24 , pp. 1-16
    • Luo, H.1    Kausch, A.P.2
  • 7
    • 0025847620 scopus 로고
    • Recombinase-mediated gene activation and site-specific integration in mammalian cells
    • O'Gorman,S., Fox,D.T. and Wahl,G.M. (1991) Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science, 251, 1351-1355.
    • (1991) Science , vol.251 , pp. 1351-1355
    • O'Gorman, S.1    Fox, D.T.2    Wahl, G.M.3
  • 9
    • 0031874849 scopus 로고    scopus 로고
    • Improved properties of flp recombinase evolved by cycling mutagenesis
    • Buchholz,F., Angrand,P.O. and Stewart,A.F. (1998) Improved properties of flp recombinase evolved by cycling mutagenesis. Nat. Biotechnol., 16, 657-662.
    • (1998) Nat. Biotechnol. , vol.16 , pp. 657-662
    • Buchholz, F.1    Angrand, P.O.2    Stewart, A.F.3
  • 10
    • 0037007010 scopus 로고    scopus 로고
    • Directed evolution of the site specificity of Cre recombinase
    • Santoro,S.W. and Schultz,P.G. (2002) Directed evolution of the site specificity of Cre recombinase. Proc. Natl Acad. Sci. USA, 99, 4185-4190.
    • (2002) Proc. Natl Acad. Sci. USA , vol.99 , pp. 4185-4190
    • Santoro, S.W.1    Schultz, P.G.2
  • 11
    • 0037423742 scopus 로고    scopus 로고
    • Stepwise manipulation of DNA specificity in Flp recombinase: Progressively adapting Flp to individual and combinatorial mutations in its target site
    • Voziyanov,Y., Konieczka,J.H., Stewart,A.F. and Jayaram,M. (2003) Stepwise manipulation of DNA specificity in Flp recombinase: progressively adapting Flp to individual and combinatorial mutations in its target site. J. Mol. Biol., 326, 65-76.
    • (2003) J. Mol. Biol. , vol.326 , pp. 65-76
    • Voziyanov, Y.1    Konieczka, J.H.2    Stewart, A.F.3    Jayaram, M.4
  • 12
    • 0036639885 scopus 로고    scopus 로고
    • Non-contact positions impose site selectivity on Cre recombinase
    • Rufer,A.W. and Sauer,B. (2002) Non-contact positions impose site selectivity on Cre recombinase. Nucleic Acids Res., 30, 2764-2771.
    • (2002) Nucleic Acids Res. , vol.30 , pp. 2764-2771
    • Rufer, A.W.1    Sauer, B.2
  • 13
    • 0034754491 scopus 로고    scopus 로고
    • Alteration of Cre recombinase site specificity by substrate-linked protein evolution
    • Buchholz,F. and Stewart,A.F. (2001) Alteration of Cre recombinase site specificity by substrate-linked protein evolution. Nat. Biotechnol., 19, 1047-1052.
    • (2001) Nat. Biotechnol. , vol.19 , pp. 1047-1052
    • Buchholz, F.1    Stewart, A.F.2
  • 14
    • 0030597357 scopus 로고    scopus 로고
    • An Escherichia coli system for assay of flp site-specific recombination on substrate plasmids
    • Snaith,M.R., Kilby,N.J. and Murray,J.A.H. (1996) An Escherichia coli system for assay of flp site-specific recombination on substrate plasmids. Gene, 180, 225-227.
    • (1996) Gene , vol.180 , pp. 225-227
    • Snaith, M.R.1    Kilby, N.J.2    Murray, J.A.H.3
  • 15
    • 0029739282 scopus 로고    scopus 로고
    • A simple assay to determine the functionality of cre or flp recombination targets in genomic manipulation constructs
    • Buchholz,F., Angrand,P.O. and Stewart,A.F. (1996) A simple assay to determine the functionality of cre or flp recombination targets in genomic manipulation constructs. Nucleic Acids Res., 24, 3118-3119.
    • (1996) Nucleic Acids Res. , vol.24 , pp. 3118-3119
    • Buchholz, F.1    Angrand, P.O.2    Stewart, A.F.3
  • 16
    • 0030472689 scopus 로고    scopus 로고
    • Toward a mechanism-based fluorescent assay for site-specific recombinases and topoisomerases - Assay design and syntheses of fluorescent substrates
    • Panigrahi,G., Zhao,B.P., Krepinsky,J.J. and Sadowski,P.D. (1996) Toward a mechanism-based fluorescent assay for site-specific recombinases and topoisomerases - assay design and syntheses of fluorescent substrates. J. Am. Chem. Soc., 118, 12004-12011.
    • (1996) J. Am. Chem. Soc. , vol.118 , pp. 12004-12011
    • Panigrahi, G.1    Zhao, B.P.2    Krepinsky, J.J.3    Sadowski, P.D.4
  • 17
    • 0031034460 scopus 로고    scopus 로고
    • Double-reciprocal crossover mediated by flp-recombinase - A concept and an assay
    • Seibler,J. and Bode,J. (1997) Double-reciprocal crossover mediated by flp-recombinase - a concept and an assay. Biochemistry, 36, 1740-1747.
    • (1997) Biochemistry , vol.36 , pp. 1740-1747
    • Seibler, J.1    Bode, J.2
  • 18
    • 0030895059 scopus 로고    scopus 로고
    • Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution
    • Schwille,P., Meyeralmes,F.J. and Rigler,R. (1997) Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys. J., 72, 1878-1886.
    • (1997) Biophys. J. , vol.72 , pp. 1878-1886
    • Schwille, P.1    Meyeralmes, F.J.2    Rigler, R.3
  • 19
    • 0002892554 scopus 로고    scopus 로고
    • Cross-correlation analysis in FCS
    • Rigler,R. and Elson,E. (eds), Springer-Verlag, Heidelberg
    • Schwille,P. (2000) Cross-correlation analysis in FCS. In Rigler,R. and Elson,E. (eds), Fluorescence Correlation Spectroscopy - Theory and Applications. Springer-Verlag, Heidelberg, pp. 360-378.
    • (2000) Fluorescence Correlation Spectroscopy - Theory and Applications , pp. 360-378
    • Schwille, P.1
  • 20
    • 0035984645 scopus 로고    scopus 로고
    • Fluorescence correlation spectroscopy for the detection and study of single molecules in Biology
    • Medina,M.A. and Schwille,P. (2002) Fluorescence correlation spectroscopy for the detection and study of single molecules in Biology. Bioessays, 24, 758-764.
    • (2002) Bioessays , vol.24 , pp. 758-764
    • Medina, M.A.1    Schwille, P.2
  • 21
    • 0037331059 scopus 로고    scopus 로고
    • Ultrasensitive investigations of biological systems by fluorescence correlation spectroscopy
    • Haustein,E. and Schwille,P. (2003) Ultrasensitive investigations of biological systems by fluorescence correlation spectroscopy. Methods, 29, 153-166.
    • (2003) Methods , vol.29 , pp. 153-166
    • Haustein, E.1    Schwille, P.2
  • 22
    • 85080354321 scopus 로고    scopus 로고
    • Evolutive Biotechnologie und Fluoreszenz-Korrelations-Spektroskopie: Systeme zur Änderung enzymatischer Substratspezifität und Auffindung neuer Wirkstoffe
    • Thesis/Dissertation, University of Hannover, Germany
    • Jahnz,M. (2003) Evolutive Biotechnologie und Fluoreszenz-Korrelations-Spektroskopie: Systeme zur Änderung enzymatischer Substratspezifität und Auffindung neuer Wirkstoffe. Thesis/Dissertation, University of Hannover, Germany.
    • (2003)
    • Jahnz, M.1
  • 23
    • 0028784138 scopus 로고
    • Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides
    • Stemmer,W.P.C., Crameri,A., Ha,K.D., Brennan,T.M. and Heyneker,H.L. (1995) Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene, 164, 49-53.
    • (1995) Gene , vol.164 , pp. 49-53
    • Stemmer, W.P.C.1    Crameri, A.2    Ha, K.D.3    Brennan, T.M.4    Heyneker, H.L.5
  • 24
    • 0022355093 scopus 로고
    • The FLP protein of the 2-micron plasmid of yeast. Purification of the protein from Escherichia coli cells expressing the cloned FLP gene
    • Babineau,D., Vetter,D., Andrews,B.J., Gronostajski,R.M., Proteau,G.A., Beatty,L.G. and Sadowski,P.D. (1985) The FLP protein of the 2-micron plasmid of yeast. Purification of the protein from Escherichia coli cells expressing the cloned FLP gene. J. Biol. Chem., 260, 12313-12319.
    • (1985) J. Biol. Chem. , vol.260 , pp. 12313-12319
    • Babineau, D.1    Vetter, D.2    Andrews, B.J.3    Gronostajski, R.M.4    Proteau, G.A.5    Beatty, L.G.6    Sadowski, P.D.7
  • 25
    • 0023664818 scopus 로고
    • Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system
    • Meyer-Leon,L., Gates,C.A., Attwood,J.M., Wood,E.A. and Cox,M.M. (1987) Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system. Nucleic Acids Res., 15, 6469-6488.
    • (1987) Nucleic Acids Res. , vol.15 , pp. 6469-6488
    • Meyer-Leon, L.1    Gates, C.A.2    Attwood, J.M.3    Wood, E.A.4    Cox, M.M.5
  • 26
    • 0029860719 scopus 로고    scopus 로고
    • Different thermostabilities of FLP and Cre recombinases: Implications for applied site-specific recombination
    • Buchholz,F., Ringrose,L., Angrand,P.O., Rossi,F. and Stewart,A.F. (1996) Different thermostabilities of FLP and Cre recombinases: Implications for applied site-specific recombination. Nucleic Acids Res., 24, 4256-4262.
    • (1996) Nucleic Acids Res. , vol.24 , pp. 4256-4262
    • Buchholz, F.1    Ringrose, L.2    Angrand, P.O.3    Rossi, F.4    Stewart, A.F.5
  • 27
    • 0022355908 scopus 로고
    • The FLP protein of the 2-micron plasmid of yeast. Inter- and intramolecular reactions
    • Gronostajski,R.M. and Sadowski,P.D. (1985) The FLP protein of the 2-micron plasmid of yeast. Inter- and intramolecular reactions. J. Biol. Chem., 260, 12328-12335.
    • (1985) J. Biol. Chem. , vol.260 , pp. 12328-12335
    • Gronostajski, R.M.1    Sadowski, P.D.2
  • 28
    • 0032573418 scopus 로고    scopus 로고
    • Comparative kinetic analysis of FLP and CRE recombinases-mathematical models for DNA binding and recombination
    • Ringrose,L., Lounnas,V., Ehrlich,L., Buchholz,F., Wade,R. and Stewart,A.F. (1998) Comparative kinetic analysis of FLP and CRE recombinases-mathematical models for DNA binding and recombination. J. Mol. Biol., 284, 363-384.
    • (1998) J. Mol. Biol. , vol.284 , pp. 363-384
    • Ringrose, L.1    Lounnas, V.2    Ehrlich, L.3    Buchholz, F.4    Wade, R.5    Stewart, A.F.6
  • 29
    • 0028799019 scopus 로고
    • A protein dissociation step limits turnover in FLP recombinase-mediated site-specific recombination
    • Waite,L.L. and Cox,M.M. (1995) A protein dissociation step limits turnover in FLP recombinase-mediated site-specific recombination. J. Biol. Chem., 270, 23409-23414.
    • (1995) J. Biol. Chem. , vol.270 , pp. 23409-23414
    • Waite, L.L.1    Cox, M.M.2
  • 31
    • 0033573989 scopus 로고    scopus 로고
    • Confocal fluorescence coincidence analysis: An approach to ultra high-throughput screening
    • Winkler,T., Kettling,U., Koltermann,A. and Eigen,M. (1999) Confocal fluorescence coincidence analysis: An approach to ultra high-throughput screening. Proc. Natl Acad. Sci. USA, 96, 1375-1378.
    • (1999) Proc. Natl Acad. Sci. USA , vol.96 , pp. 1375-1378
    • Winkler, T.1    Kettling, U.2    Koltermann, A.3    Eigen, M.4
  • 32
    • 0032539688 scopus 로고    scopus 로고
    • Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy
    • Kettling,U., Koltermann,A., Schwille,P. and Eigen,M. (1998) Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy. Proc. Natl Acad. Sci. USA, 95, 1416-1420.
    • (1998) Proc. Natl Acad. Sci. USA , vol.95 , pp. 1416-1420
    • Kettling, U.1    Koltermann, A.2    Schwille, P.3    Eigen, M.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.