An Escherichia coli system for assay of Flp site-specific recombination on substrate plasmids

Gene

Volumn 180, Issue 1-2, 1996, Pages 225-227

  Snaith, Michael R ^a   Kilby, Nigel J ^a   Murray, James A H ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

2 m circle plasmid; Bacteriophage T7 RNA polymerase; FLP; Recombinant DNA tools; Yeast site specific recombinase

Indexed keywords

KANAMYCIN; PLASMID DNA; RECOMBINASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIOPHAGE T7; DNA RECOMBINATION; DNA REPLICATION ORIGIN; ENZYME ASSAY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; RECOMBINANT GENE;

BINDING SITES; DNA NUCLEOTIDYLTRANSFERASES; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINATION, GENETIC;

ESCHERICHIA COLI;

EID: 0030597357     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00449-0     Document Type: Article

References (6)

1. Broach, J.R., Guarascio, V.R. and Jayaram, M. (1982) Recombination within the yeast 2-μm circle is site-specific. Cell 29, 227-234.
2. Elledge, S.J., Mulligan, J.T., Ramer, S.W., Spottswood, M. and Davis, R.W. (1991) λYES: A multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88, 1731-1735.
3. Kilby, N.J., Snaith, M.R. and Murray, J.A.H. (1993) Site-specific recombinases: Tools for genome engineering. Trends Genet. 9, 413-421.
4. O'Gorman, S., Fox, D.T. and Wahl G.M. (1991) Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science 251, 1351-1355.
5. Poutska, A., Rackwitz, H.-R., Frischauf, A.-M., Hohn, B. and Lehrach, H. (1984) Selective isolation of cosmid clones by homologous recombination in Escherichia coli. Proc. Natl. Acad. Sci. USA 81, 4129-4133.
6. Tabor, S. and Richardson, C.C. (1985) A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82, 1074-1078.