Analysis of Ras and Rap activation in living cells using fluorescent Ras binding domains

Methods

Volumn 37, Issue 2, 2005, Pages 138-145

  Bivona, Trever G ^a   Philips, Mark R ^a  

^a NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

FRET; GFP; Rap1; Ras; Ras binding domain (RBD)

Indexed keywords

GUANOSINE TRIPHOSPHATASE; RAP PROTEIN; RAS PROTEIN;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL ASSAY; CONTROLLED STUDY; ENZYME ACTIVATION; EQUIPMENT; FEMALE; FLUORESCENCE; HUMAN; HUMAN CELL; NONHUMAN; PATHOLOGY; PHYSIOLOGY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FUNCTION; SENSOR; TECHNOLOGY;

ANIMALS; BINDING SITES; CERCOPITHECUS AETHIOPS; COS CELLS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HELA CELLS; HUMANS; JURKAT CELLS; PROTEIN STRUCTURE, TERTIARY; RAP GTP-BINDING PROTEINS; RAS PROTEINS; STAINING AND LABELING;

ANIMALIA;

EID: 27744571333     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2005.05.022     Document Type: Article

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