Imaging the intracellular trafficking and state of the AB5 quaternary structure of cholera toxin

EMBO Journal

Volumn 15, Issue 16, 1996, Pages 4246-4253

  Bastiaens, Philippe I H ^a   Majoul, Irina V ^b   Verveer, Peter J ^a   Söling, Hans Dieter ^b   Jovin, Thomas M ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

ADP ribosylation; Confocal microscopy; Fluorescence resonance energy transfer; Photobleaching

Indexed keywords

CHOLERA TOXIN; FLUORESCENT DYE;

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; GOLGI COMPLEX; NONHUMAN; PRIORITY JOURNAL; PROTEIN QUATERNARY STRUCTURE; VERO CELL;

EID: 0029741379     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00799.x     Document Type: Article

References (29)

1. Bastiaens, P.I.H. and Jovin, T.M. (1996) Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein: fluorescent labeled protein kinase C βI. Proc. Natl Acad. Sci. USA, in press.
2. Bastiaens, P.I.H., Wouters, F.S. and Jovin, T.M. (1996) Imaging the molecular state of proteins in cells by fluorescence resonance energy transfer (FRET). Sequential photobleaching of Förster donor-acceptor pairs. Proceedings of the Second Hamamatsu International Symposium on Biomolecular Mechanisms and Photanics: Cell-Cell Communication. Hamamatsu Photonics, K.K., in press.
3. Carrington, W.A., Lynch, R.M., Moore, E.D.W., Isenberg, G., Fogarty, K.E. and Fay, F.S. (1995) Superresolution three-dimensional images of fluorescence in cells with minimal light exposure. Science, 268, 1483-1487.
4. Cassel, D. and Pfeuffer, T. (1978) Mechanism of cholera toxin action: covalent modification of the guanyl nucleotide-binding protein of the adenylate cyclase system. Proc. Natl Acad. Sci. USA. 75, 2669-2673.
5. Cassel, D. and Selinger, Z. (1977) Mechanism of adenylate cyclase activation by cholera toxin: inhibition of GTP hydrolysis at the regulatory site. Proc. Natl Acad. Sci. USA, 74, 3307-3311.
6. Clegg, R.M. (1995) Fluorescence resonance energy transfer. Curr. Opin. Biotechnol., 6, 103-110.
7. Demandolx, D. and Davoust, J. (1996) Subcellular cytofluorometry in confocal microscopy. In Slavik, J. (ed.). Fluorescence Microscopy and Fluorescence Probes. Plenum Press, New York, in press.
8. Field, M., Rao, M.C. and Chang, E.B. (1989) Intestinal electrolyte transport and diarrheal disease 1. N. Engl. J. Med., 321, 800-806.
9. Förster, T. (1948) Zwischenmolekulare Energiewanderung und Fluoreszenz. Ann. Physik., 2, 55-75.
10. Gadella, T.W.J., Jr and Jovin, T.M. (1995) Oligomerization of epidermal growth factor receptors (EGFR) on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation. J. Cell Biol., 129, 1543-1558.
11. Gadella, T.W.J., Jr. Jovin, T.M. and Clegg, R.M. (1993) Fluorescence lifetime imaging microscopy (FLIM): spatial resolution of microstructures on the nanosecond time scale. Biophys. Chem., 48, 221-239.
12. Gadella, T.W.J., Jr. Clegg, R.M. and Jovin, T.M. (1994) Fluorescence lifetime imaging microscopy: pixel-by-pixel analysis of phase-modulation data. Bioimaging, 2, 139-159.
13. + by cholera toxin. Biochem. J., 244, 225-230.
14. Gill, D.M. (1976) The arrangement of subunits of cholera toxin. Biochemistry, 15, 1242-1248.
15. Gill, D.M. (1977) Mechanism of action of cholera toxin. Adr. Cyclic Nucleotide Res., 8, 85-118.
16. Jovin, T.M. and Arndt-Jovin, D.J. (1989) FRET microscopy: digital imaging of fluorescence resonance energy transfer. Application in cell biology. In Kohen, E., Ploem, J.S. and Hirschberg, J.G. (eds), Cell Structure and Function by Microspectrofluorimetry. Academic Press, Orlando, FL, pp. 99-117.
17. Lencer, W.I., Moe, S., Rufo, P.A. and Madara, J.L. (1995) Transcytosis of cholera toxin subunits across model human intestinal epithelia. Proc. Natl Acad. Sci. USA, 92, 10094-10098.
18. Majoul, I.V., Bastiaens, P.I.H. and Söling, H.D. (1996) Transport of an external KDEL-protein from the plasma membrane to the endoplasmic reticulum. Studies with cholera toxin in Vero cells. J. Cell Biol., 133, 777-789.
19. Mekalanos, J.J., Collier, R.J. and Romig, W.R. (1979) Enzymic activity of cholera toxin. II Relationship to proteolytic processing, disulfide bond reduction and subunit composition. J. Biol. Chem., 254, 5855-5861.
20. Nambiar, M.P., Oda, T., Chen, C. Kuwazuru, Y. and Wu, H.C. (1993) Involvement of the Golgi region in the intracellular trafficking of cholera toxin. J. Cell Physiol., 154, 222-228.
21. Orlandi, P.A., Curran, P.K. and Fishman, P.H. (1993) Brefeldin A blocks the response of cultured cells to cholera toxin. Implications for intracellular trafficking in toxin action. J. Biol. Chem., 268, 12010-12016.
22. Parton, R.G. (1994) Ultrastructural localization of gangliosides; GM1 is concentrated in caveoli. J. Histochem. Cytochem., 42, 155-166.
23. Parton, R.G., Joggerst, B. and Simons, K. (1994) Regulated internalization of caveolae. J. Cell Biol., 127, 1199-1215.
24. Sofer, A. and Futerman, A.H. (1995) Cationic amphiphilic drugs inhibit the internalization of cholera toxin to the Golgi apparatus and the subsequent elevation of cyclic AMP. J. Biol. Chem., 270, 12117-12122.
25. Southwick, P.L., Ernst, L.A., Tauriello, E.W., Parker, S.R., Mujumdar, R.B., Mujumdar, S.R., Clever, H.A. and Waggoner, A.S. (1990) Cyanine dye labeling reagents-carboxymethylindocyanine succinimidyl esters. Cytometry, 11, 418-430.
26. Spangler, B.D. (1992) Structure and function of cholera toxin and the related Escherichia coli heat-labile enterotoxin. Microbiol. Rev., 56, 622-647.
27. Tran, D., Carpentier, J.L., Sawano, F., Gordon, P. and Orci, L. (1987) Ligand internalized through coated or noncoated invaginations follow a common intracellular pathway. Proc. Natl Acad. Sci. USA, 84, 7957-7961.
28. Tsien, R.Y., Bacskai, B.J. and Adams, S.R. (1993) FRET for studying intracellular signalling. Trends Cell Biol., 3, 242-245.
29. 1. Science, 183, 656-657.