Characterization of cells with different mitochondrial membrane potential during apoptosis

Cytometry Part A

Volumn 68, Issue 1, 2005, Pages 28-35

  Lugli, Enrico ^a   Troiano, Leonarda ^a   Ferraresi, Roberta ^a   Roat, Erika ^a   Prada, Nicole ^b   Nasi, Milena ^a   Pinti, Marcello ^a   Cooper, Edwin L ^c   Cossarizza, Andrea ^a,d  

^a UNIVERSITY OF MODENA AND REGGIO EMILIA   (Italy)

^b UNIVERSITY OF PALERMO   (Italy)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d Section of General Pathology   (Italy)

Author keywords

Annexin V; Apoptosis; Hoechst; JC 1; Mitochondrial membrane potential; Polychromatic flow cytometry; Propidium iodide

Indexed keywords

5,5',6,6' TETRACHLORO 1,1',3,3' TETRAETHYLBENZIMIDAZOLECARBOCYANINE IODIDE; DNA; FLAVONOID; FLUORESCENT DYE; HOE 33342; LIPOCORTIN 5; MITOTRACKER DEEP RED; PHOSPHATIDYLSERINE; PROPIDIUM IODIDE; QUERCETIN; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL DEATH; CELL POPULATION; CELL STRAIN U937; CONTROLLED STUDY; DNA CONTENT; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LASER; MEMBRANE PERMEABILITY; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; PHYSICAL PARAMETERS; PRIORITY JOURNAL; STAINING; ULTRAVIOLET RADIATION;

APOPTOSIS; BENZIMIDAZOLES; CARBOCYANINES; CELL LINE, TUMOR; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; DAUNORUBICIN; DNA; DNA FRAGMENTATION; DOXORUBICIN; FLOW CYTOMETRY; FLUORESCENT DYES; HL-60 CELLS; HUMANS; INTRACELLULAR MEMBRANES; LEUKOCYTES, MONONUCLEAR; MEMBRANE POTENTIALS; MITOCHONDRIA; PHOSPHATIDYLSERINES; QUERCETIN; U937 CELLS;

EID: 27644558367     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.20188     Document Type: Article

References (26)

1. Green DR, Reed JC. Mitochondria and apoptosis. Science 1998;281:1309-1312.
2. Green DR, Kroemer G. The pathophysiology of mitochondrial cell death. Science 2004;305:626-629.
3. Cossarizza A, Kalashnikova G, Grassilli E, Chiappelli F, Salvioli S, Capri M, Barbieri D, Troiano L, Monti D, Franceschi C. Mitochondrial modifications during rat thymocyte apoptosis: a study at the single cell level. Exp Cell Res 1994;214:323-330.
4. Richter C, Schweizer M, Cossarizza A, Franceschi C. Control of apoptosis by the cellular ATP level. FEBS Lett 1996;378:107-110.
5. Petit PX, Susin SA, Zamzami N, Mignotte B, Kroemer G. Mitochondria and programmed cell death: back to the future. FEBS Lett 1996;396:7-13.
6. Bossy-Wetzel E, Newmeyer DD, Green DR. Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVD-specific caspase activation and independently of mitochondrial transmembrane depolarization. Embo J 1998;17:37-49.
7. Kroemer G, Zamzami N, Susin SA. Mitochondrial control of apoptosis. Immunol Today 1997;18:44-51.
8. 6(3) or rhodamine 123, is a reliable fluorescent probe to assess ΔΨ changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett 1997;411:77-82.
9. Cossarizza A, Baccarani-Contri M, Kalashnikova G, Franceschi C. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5.5′, 6,6′- tetrachloro-1,1′, 3,3′-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem Biophys Res Commun 1993;197:40-45.
10. Salvioli S, Dobrucki J, Moretti L, Troiano L, Fernandez MG, Pinti M, Pedrazzi J, Franceschi C, Cossarizza A. Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60 cells: analysis at the single cell and single organelle level. Cytometry 2000;40:189-197.
11. Rong Y, Yang EB, Zhang K, Mack P. Quercetin-induced apoptosis in the monoblastoid cell line U937 in vitro and the regulation of heat shock proteins expression. Anticancer Res 2000;20:4339-4345.
12. Mazzini G, Ferrari C, Erba E. Dual excitation multi-fluorescence flow cytometry for detailed analyses of viability and apoptotic cell transition. Eur J Histochem 2003;47:289-298.
13. Barbieri D, Troiano L, Grassilli E, Agnesini C, Cristofalo EA, Monti D, Capri M, Cossarizza A, Franceschi C. Inhibition of apoptosis by zinc: a reappraisal. Biochem Biophys Res Commun 1992;187:2156-2161.
14. Brusselmans K, Vrolix R, Verhoeven G, Swinnen JV. Induction of cancer cell apoptosis by flavonoids is associated with their ability to inhibit fatty acid synthase activity. J Biol Chem 2005;280:5636-5645.
15. Kuo PC, Liu HF, Chao JL. Survivin and p53 modulate quercetin-induced cell growth inhibition and apoptosis in the human lung carcinoma cells. J Biol Chem 2004;279:55875-55885.
16. Chen YC, Shen SC, Chow JM, Ko CH, Tseng SW. Flavone inhibition of tumor growth via apoptosis in vitro and in vivo. Int J Oncol 2004;25:661-670.
17. Ferraresi R, Troiano L, Roat E, Lugli E, Nemes E, Nasi M, Pinti M, Garcia Fernandez MI, Cooper EL, Cossarizza A. Essential requirement of reduced glutathione for the anti-oxidant effect of the flavonoid quercetin. Free Radic Res (in press).
18. Danial NN, Gramm CF, Scorrano L, Zhang CY, Krauss S, Ranger AM, Datta SR, Greenberg ME, Licklider LJ, Lowell BB, Gygi SP, Korsmeyer SJ. BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 2003;424:952-956.
19. Saleh A, Srinivasula SM, Balkir L, Robbins PD, Alnemri ES. Negative regulation of the Apaf-1 apoptosome by Hsp70. Nat Cell Biol 2000;2:476-483.
20. Ferrari D, Stepczynska A, Los M, Wesselborg S, Schulze-Osthoff K. Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis. J Exp Med 1998;188:979-984.
21. Zamzami N, Marchetti P, Castedo M, Zanin C, Vayssiere JL, Petit PX, Kroemer G. Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo. J Exp Med 1995;181:1661-1672.
22. m) in apoptosis: an update. Apoptosis 2003;8:115-128.
23. Kluck RM, Bossy-Wetzel E, Green DR, Newmeyer DD. The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis. Science 1997;275:1132-1136.
24. Yang J, Liu X, Bhalla K, Kim CN, Ibrado AM, Cai J, Peng TI, Jones DP, Wang X. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria Blocked. Science 1997;275:1129-1132.
25. Minamikawa T, Williams DA, Bowser DN, Nagley P. Mitochondrial permeability transition and swelling can occur reversibly without inducing cell death in intact human cells. Exp Cell Res 1999;246:26-37.
26. Salvioli S, Barbi C, Dobrucki J, Moretti L, Pinti M, Pedrazzi J, Pazienza TL, Bobyleva V, Franceschi C, Cossarizza A. Opposite role of changes in mitochondrial membrane potential in different apoptotic processes. FEBS Lett 2000;469:186-190.