In vitro digestion and fermentation methods, including gas production techniques, as applied to nutritive evaluation of foods in the hindgut of humans and other simple-stomached animals

Animal Feed Science and Technology

Volumn 123-124 Part 1, Issue , 2005, Pages 421-444

  Coles, L T ^a   Moughan, P J ^a   Darragh, A J ^b  

^a MASSEY UNIVERSITY   (New Zealand)

^b MASSEY UNIVERSITY   (New Zealand)

Author keywords

Digestibility; Fermentation; Gas production; Hindgut; Monogastric

Indexed keywords

ANIMALIA; BOVIDAE;

EID: 27544443243     PISSN: 03778401     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.anifeedsci.2005.04.021     Document Type: Article

References (125)

1. J. Adiotomre, M.A. Eastwood, C.A. Edwards, and W. Brydon Dietary fibre: in vitro methods that anticipate nutrition and metabolic activity in humans Am. J. Clin. Nutr. 52 1990 128 134
2. Ahrens, F., Schön, J., Schmitz, M., 1991. A discontinuous in vitro technique for measuring hind gut fermentation in pigs. In: Verstegen, M.W.A., Huisman, J., den Hartog, L.A. (Eds.), Digestive Physiology in Pigs. EAAP Publication no. 54. Pudoc, Wageningen, The Netherlands, pp. 226-230.
3. P. Åman, J.X. Zhang, G. Hallmans, and E. Lundin Excretion and degradation of dietary fiber constituents in ileostomy subjects consuming a low fiber diet with and without brewer's spent grain J. Nutr. 124 1994 359 363
4. A.M. Aura, H. Härkönen, M. Fabritius, and K. Poutanen Development of an in vitro enzymatic digestion method for removal of starch and protein and assessment of its performance using rye and wheat breads J. Cereal Sci. 29 1999 139 152
5. K.E. Bach Knudsen, E. Wisker, M. Daniel, W. Feldheim, and B.O. Eggum Digestibility of energy, protein, fat and non-starch polysaccharides in mixed diets: comparative studies between man and the rat Br. J. Nutr. 71 1994 471 487
6. J.L. Barry, C. Hoebler, G.T. Macfarlane, S. Macfarlane, J.C. Mathers, K.A. Reed, P.B. Mortensen, I. Nordgaard, I.R. Rowland, and C.J. Rowland Estimation of the fermentability of dietary fibre in vitro: a European interlaboratory study Br. J. Nutr. 74 1995 303 322
7. E. Bauer, B.A. Williams, C. Voigt, R. Mosenthin, and M.W.A. Verstegen Microbial activities of faeces from unweaned and adult pigs, in relation to selected fermentable carbohydrates Anim. Sci. 73 2001 313 322
8. E. Bauer, B.A. Williams, C. Voigt, R. Mosenthin, and M.W.A. Verstegen Impact of mammalian enzyme pretreatment on the fermentability of carbohydrate-rich feedstuffs J. Sci. Food. Agric. 83 2003 207 214
9. R.M. Beames, J.H. Helm, B.O. Eggum, S. Boisen, K.E. Bach Knudsen, and M.L. Swift A comparison of methods for measuring the nutritive value for pigs of a range of hulled and hulless barley cultivars Anim. Feed Sci. Technol. 62 1996 189 201
10. P.M. Becker, A.H. van Gelder, P.G. van Wikselaar, A.W. Jongbloed, and J.W. Cone Carbon balances for in vitro digestion and fermentation of potential roughages for pregnant sows Anim. Feed Sci. Technol. 110 2003 159 174
11. A.M. Birkett, J.C. Mathers, G.P. Jones, K.Z. Walker, M.J. Roth, and J.G. Muir Changes to the quantity and processing of starchy foods in a Western diet can increase polysaccharides escaping digestion and improve in vitro fermentation variables Br. J. Nutr. 84 2000 63 72
12. S. Boisen In vitro digestibility methods: history and specific approaches P.J. Moughan M.W.A. Verstegen M.I. Visser-Reyneveld Feed Evaluation: Principles and Practice 2000 Wageningen Pers Wageningen, The Netherlands
13. S. Boisen, and B.O. Eggum Critical evaluation of in vitro methods for estimating digestibility in simple-stomached animals Nutr. Res. Rev. 4 1991 141 162
14. S. Boisen, and J.A. Fernández In vitro digestibility of energy and amino acids in pig feeds M.W.A. Verstegen J. Huisman L.A. den Hartog Digestive Physiology in the Pig 1991 Pudoc Wageningen, The Netherlands 231 236
15. S. Boisen, and J.A. Fernández Prediction of the total tract digestibility of energy in feedstuffs and pig diets by in vitro analyses Anim. Feed Sci. Technol. 68 1997 277 286
16. Bosch, M.W., Williams, B.A., Bos, L.W., Verstegen, M.W.A., 2002. In vitro fermentability of carbohydrates by faecal microflora of pigs at weaning. In: Proceedings of the 3rd Rowett/INRA Symposium on Beyond Antimicrobials - The Future of Gut Microbiology, 12-15 June 2002. Aberdeen, UK, p. 62.
17. Boudry, C., Estrada, F., Schoeling, O., Froidmont, E., Wavreille, J., Buldgen, A., 2003. Interest of in vitro pre-digestion to estimate fermentability of feedstuffs in pig large intestine. In: Proceedings of the 9th International Symposium on Digestive Physiology in Pigs, vol. 2, Banff, AB, Canada.
18. L.D. Bourquin, E.C. Titgemeyer, K.A. Garleb, and G.C. Fahey Jr. Shortchain fatty acid production and fiber degradation by human colonic bacteria: effects of substrate and cell wall fractionation procedures J. Nutr. 122 1992 1508 1520
19. L.D. Bourquin, E.C. Titgemeyer, and G.C. Fahey Jr. Fermentation of various dietary fiber sources by human fecal bacteria Nutr. Res. 16 1996 1119 1131
20. G. Breves, and K. Stuck Short-chain fatty acids in the hindgut J.H. Cummings J.L. Rombeau T. Sakata Physiological and Clinical Aspects of Short-chain Fatty Acids 1995 Cambridge University Press Cambridge, UK 73 85
21. G. Breves, E. Schulze, H.P. Sallmann, and D. Gadeken The application of 13C labelled short chain fatty acids to measure acetate and propionate production rates in the large intestines: studies in a pig model Z. Gastroenterol. 31 1993 179 182
22. P. Brøbech Mortensen, H. Hove, M. Rye Clausen, and K. Holtug Fermentation to short-chain fatty acids and lactate in human faecal batch cultures intra- and inter-individual variations versus variations caused by changes in fermented saccharides Scand. J. Gastroenterol. 26 1991 1285 1294
23. N.R. Bullock, and G. Norton Biotechniques to assess the fermentation of resistant starch in the mammalian gastrointestinal tract Carbohydr. Polym. 38 1999 225 230
24. D.P. Burkitt, A.R.P. Walker, and N.S. Painter Effect of dietary fibre on stools and transit times, and its role in the causation of disease Lancet 2 1972 1408 1412
25. S. Calabró, A. Nizza, W. Pinna, M.I. Cutrignelli, and V. Piccolo Estimation of digestibility of compound diets for rabbits using the in vitro gas production technique World Rabbit Sci. 74 1999 197 201
26. J.M. Campbell, and G.C. Fahey Jr. Psyllium and methylcellulose fermentation properties in relation to insoluble and soluble fiber standards Nutr. Res. 17 1997 619 629
27. J.L. Casterline Jr., C.J. Oles, and Y. Ku In vitro fermentation of various food fiber fractions J. Agric. Food Chem. 45 1997 2463 2467
28. A. Chako, and J.H. Cummings Nitrogen losses from the small bowel: obligatory losses and the effect of physical form Gut 29 1988 608 613
29. Chen, J., 1997. Prediction of the in vivo digestible energy value of barley for the growing pig on the basis of physical and chemical characteristics and in vitro digestible energy. MAgSci Thesis. Massey University, Palmerston North, New Zealand.
30. D.N. Christensen, K.E. Bach-Knudsen, J. Wolstrup, and B.B. Jensen Integration of ileum cannulated pigs and in vitro fermentation to quantify the effect of diet composition on the amount of short-chain fatty acids available for fermentation in the large intestine J. Sci. Food Agric. 79 1999 755 762
31. S.K. Clinton, D.G. Bostwick, L.M. Olson, H.J. Mangian, and W.J. Wisek Effects of ammonium acetate and sodium cholate on N-methyl-N′-nitro-N- nitrosoguanidine-induced colon carcinogenesis of rats Cancer Res. 48 1988 3035 3039
32. J.W. Cone, A.H. van Gelder, G.J.W. Visscher, and L. Oudshoorn Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus Anim. Feed Sci. Technol. 61 1996 113 128
33. J.H. Cummings Fermentation in the human large intestine: evidence and implications for health Lancet 1 1983 1206 1209
34. J.H. Cummings The Large Intestine in Nutrition and Disease. Danone Chair Monograph 1996 Institute Danone Brussels, Belgium 155 pp
35. J.H. Cummings, and G.T. Macfarlane The control and consequences of bacterial fermentation in the human colon J. Appl. Bacteriol. 70 1991 443 459
36. J.H. Cummings, S.A. Bingham, K.W. Heaton, and M.A. Eastwood Fecal weight, colon cancer risk and dietary intake of non-starch polysaccharides (dietary fiber) Gastroenterology 103 1992 1783 1789
37. J. Daly, J. Tomlin, and N.W. Read The effect of feeding xanthan gum on colonic function in man: correlation with in vitro determinant of bacterial breakdown Br. J. Nutr. 69 1993 897 902
38. M. Daniel, E. Wisker, G. Rave, and W. Feldheim Fermentation in human subjects of nonstarch polysaccharides in mixed diets, but not in barley fiber concentrate, could be predicted by in vitro fermentation using human fecal inocula J. Nutr. 127 1997 1981 1988
39. J.W. Dobbins, and H.J. Binder Pathophysiology of diarrhoea: alterations in fluid and electrolyte transport J. Clin. Gastroenterol. 10 1981 605 626
40. B.S. Drasar, and M.J. Hill Human Intestinal Flora 1974 Academic Press London, UK
41. C.A. Edwards, G. Gibson, M. Champ, B.-B. Jensen, J.C. Mathers, F. Nagengast, C. Rumney, and A. Quehl In vitro method for quantification of the fermentation of starch by human faecal bacteria J. Sci. Food Agric. 71 1996 209 217
42. F.R. Ehle, J.L. Jeraci, J.B. Roberston, and P.J. Van Soest The influence of dietary fibre on digestibility, rate of passage and gastrointestinal fermentation in pigs J. Anim. Sci. 55 1982 1071 1081
43. W.N. Ewing, and D.J.A. Cole The Living Gut: An Introduction to Micro-organisms in Nutrition 1994 Context Dungannon, Ireland
44. A. Fardet, F. Guillon, C. Hoebler, and J.L. Barry In vitro fermentation of beet fibre and barley bran, of their insoluble residues after digestion and of ileal effluents J. Sci. Food Agric. 75 1997 315 325
45. J. Fernandes, A.V. Rao, and T.M.S. Wolever Different substrates and methane producing status affect short-chain fatty acid profiles produced by in vitro fermentation of human faeces J. Nutr. 130 2000 1932 1936
46. S.M. Finegold, and V.L. Sutter Fecal flora in different populations, with special reference to diet Am. J. Clin. Nutr. 31 1978 S116 S122
47. S.M. Finegold, V.L. Sutter, and G.E. Mattisen Normal indigenous intestinal flora D.J. Hentges Human Intestinal Microflora in Health and Disease 1983 Academic Press New York, NY, USA 3 31
48. M. Fondevilla, J. Morales, J.F. Pérez, A. Barrios-urdaneta, and M.D. Baucells Microbial caecal fermentation in Iberic or Landrace pigs given acorn/sorghum or maize diets estimated in vitro using the gas production technique Anim. Feed Sci. Technol. 102 2002 93 107
49. J. France, J. Dijkstra, M.S. Dhanoa, S. Lopez, and A. Bannink Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: derivation of models and other mathematical considerations Br. J. Nutr. 83 2000 143 150
50. K. Freeman, T. Foy, A.S. Feste, P.J. Reeds, and C.L. Lifschitz Colonic acetate in the circulating acetate pool of the infant pig Paediatr. Res. 34 1993 318 322
51. M.F. Fuller In Vitro Digestion for Pigs and Poultry 1991 CAB International Wallingford, UK
52. S. Furuya, K. Sakamoto, and S. Takahashi A new in vitro method for the estimation of digestibility using intestinal fluid of the pig Br. J. Nutr. 41 1979 511 520
53. Goering, H.K., Van Soest, P.J., 1970. Forage fibre analyses. U.S. Department of Agriculture Handbook no. 379. U.S. Government Printing Office, Washington, DC, USA.
54. I. Goñi, and N. Martin-Carrón In vitro fermentation and hydration properties of commercial dietary fiber-rich supplements Nutr. Res. 6 1998 1077 1089
55. I. Goñi, M. Gudiel-Urbano, and F. Saura-Calixto In vitro determination of digestible and unavailable protein in edible seaweeds J. Sci. Food Agric. 82 2002 1850 1854
56. H. Graham, W. Löwgren, and P. Åman An in vitro method for studying digestion in the pig 2. Comparison with in vivo ileal and faecal digestibilities Br. J. Nutr. 61 1989 689 698
57. M. Granito, M. Champ, A. David, C. Bonnet, and M. Guerra Identification of gas-producing components in different varieties of Phaseolus vulgaris by in vitro fermentation J. Sci. Food Agric. 81 2001 543 550
58. F. Guillon, J.L. Barry, and J.F. Thibault Effect of autoclaving sugar-beet fibre on its physico-chemical properties and its in-vitro degradation by human faecal flora J. Sci. Food Agric. 60 1992 69 79
59. F. Guillon, C. Renard, J. Hospers, J.F. Thibault, and J.L. Barry Characterisation of residual fibres from fermentation of pea and apple fibres by human colonic bacteria J. Sci. Food Agric. 68 1995 521 529
60. R. Havenaar, and M. Minekus Simulated assimilation Dairy Ind. 61 9 1996 17 21
61. C. Hoebler, F. Guillon, A. Fardet, C. Cherbut, and J.L. Barry Gastrointestinal or simulated in vitro digestion changes dietary fibre properties and their fermentation J. Sci. Food Agric. 77 1998 327 333
62. Karppinen, S., 2003. Dietary fibre components of rye bran and their fermentation in vitro. Ph.D. Thesis. VTT Biotechnology, Finland.
63. S. Karppinen, K. Liukkonen, A.M. Aura, P. Forssell, and K. Poutanen In vitro fermentation of polysaccharides of rye, wheat and oat brans and inulin by human faecal bacteria J. Sci. Food Agric. 80 2000 1469 1476
64. A.M. Langkilde, M. Champ, and H. Andersson Effects of high-resistant-starch banana flour (RS2) on in vitro fermentation and the small-bowel excretion of energy, nutrients, and sterols: an ileostomy study Am. J. Clin. Nutr. 75 2002 104 111
65. V. Lebet, E. Arrigoni, and R. Amado Measurement of fermentation products and substrate disappearance during incubation of dietary fibre sources with human faecal flora Lebensm. Wiss. u. Technol. 31 1998 473 479
66. V. Lebet, E. Arrigoni, and R. Amado Digestion procedure using mammalian enzymes to obtain substrates for in vitro fermentation studies Lebensm. Wiss. u. Technol. 31 1998 509 515
67. W. Löwgren, H. Graham, and P. Åman An in vitro method for studying digestion in the pig 1. Simulating digestion in the different compartments of the intestine Br. J. Nutr. 61 1989 673 687
68. R.S. Lowman, M.K. Theodorou, J.J. Hyslop, M.S. Dhanoa, and D. Cuddeford Evaluation of an in vitro batch culture technique for estimating the in vivo digestibility and digestible energy content of equine feeds using equine faeces as the source of microbial inoculum Anim. Feed Sci. Technol. 80 1999 11 27
69. G.T. Macfarlane, and J.H. Cummings The colonic flora, fermentation, and large bowel digestive function S.F. Phillips J.H. Pemberton R.G. Shorter The Large Intestine: Physiology, Pathophysiology and Disease 1991 Raven Press New York, NY, USA 51 92
70. G.T. Macfarlane, G.R. Gibson, B.S. Drasar, and J.H. Cummings Metabolic significance of the gut microflora R. Whitehead Gastrointestinal Oesophageal Pathology second ed. 1995 Churchill Livingstone Edinburgh, UK 249 274
71. R.M. Mauricio, E. Owen, M.S. Dhanoa, and M.K. Theodorou Comparison of rumen liquor and faeces from cows as source of microorganisms for the in vitro gas production technique Proceedings of the Occasional Meeting of the British Society of Animal Science on In Vitro Techniques for Measuring Nutrient Supply to Ruminants University of Reading, UK 1997
72. M.I. McBurney, and L.U. Thompson Dietary fiber and energy balance: integration of the human ileostomy and in vitro fermentation models Anim. Feed. Sci. Technol. 23 1989 261 275
73. M.I. McBurney, and P.J. Van Soest Structure-function relationships S.F. Phillips J.H. Pemberton R.G. Shorter The Large Intestine: Physiology, Pathophysiology and Disease 1991 Raven Press New York, NY, USA 37 49
74. M.I. McBurney, and W.C. Sauer Fiber and large bowel energy absorption: validation of the integrated ileostomy-fermentation model using pigs J. Nutr. 123 1993 721 727
75. M.I. McBurney, L.U. Thompson, and D.J.A. Jenkins Colonic fermentation of some breads and its implications for energy availability in man Nutr. Res. 7 1987 1229 1241
76. N.I. McNeil The contribution of the large intestine to energy supplies in man Am. J. Clin. Nutr. 39 1984 338 342
77. K.H. Menke, and H. Steingass Estimation of the energetic feed value obtained from chemical analyses and in vitro gas production using rumen fluid Anim. Res. Dev. 28 1988 7 55
78. K.H. Menke, L. Raab, A. Salewski, H. Steingass, D. Fritz, and W. Schneider The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro J. Agric. Sci. (Camb.) 93 1979 217 222
79. A. Metcalfe, S.F. Phillips, A.R. Zinsmeister, R.L. MacCarty, R.W. Beart, and B.G. Wolff Simplified assessment of segmental colonic transit Gastroenterology 92 1987 40 47
80. M. Minekus, M. Smeets-Peeters, A. Bernalier, S. Marol-Bonnin, R. Havenaar, P. Marteau, M. Alric, G. Fonty, and J.H. Huis in't Veld A computer-controlled system to simulate conditions of the large intestine with peristaltic mixing, water absorption and absorption of fermentation products Appl. Microbiol. Biotechnol. 53 1999 108 114
81. D.J. Monsma, and J.A. Marlett Rat cecal inocula produce different patterns of short-chain fatty acids than fecal inocula in in vitro fermentations J. Nutr. 125 1995 2463 2470
82. D.J. Monsma, and J.A. Marlett Fermentation of carbohydrate in rat ileal excreta is enhanced with cecal inocula compared with fecal inocula J. Nutr. 126 1996 554 563
83. D.J. Monsma, P.T. Thorsen, N.W. Vollendorf, T.D. Crenshaw, and J.A. Marlett In vitro fermentation of swine ileal digesta containing oat bran dietary fiber by rat cecal inocula adapted to the test fiber increases propionate production but fermentation of wheat bran ileal digesta does not produce more butyrate J. Nutr. 130 2000 585 593
84. M.J.S. Moore-Colyer, and A.C. Longland The in vitro digestion of mature grass hay in the presence or absence of added nitrogen and sugar beet pulp by an equine faecal inoculum using the pressure transducer technique Proceedings of the British Society of Animal Science Winter Meeting Scarborough, UK 2001 124
85. P.J. Moughan In vitro techniques for the assessment of the nutritive value of feed grains for pigs: a review Aust. J. Agric. Res. 50 1999 871 879
86. P.J. Moughan Amino acid availability: aspects of chemical analysis and bioassay methodology Nutr. Res. Rev. 16 2003 127 141
87. J.M.D. Murray, M.J.S. Moore-Colyer, A.C. Longland, and C. Dunnett The use of faecal inocula for estimating the in vitro digestibility of horse feeds Proceedings of the British Society of Animal Science Winter Meeting York, UK 2003 48
88. M. Nyman, N.-G. Asp, J. Cummings, and H. Wiggins Fermentation of dietary fibre in the intestinal tract: comparison between man and rat Br. J. Nutr. 55 1986 487 496
89. J.W. Payne Transport of peptides in microorganisms D.M. Matthews J.W. Payne Peptide Transport in Protein Nutrition, The Frontiers of Biology Series vol. 37 1975 North Holland Publishing Company Amsterdam, The Netherlands 283 364
90. Å. Pettersson, J.E. Lindberg, S. Thomke, and B.O. Eggum Nutrient digestibility and protein quality of oats differing in chemical composition evaluated in rats and by an in vitro technique Anim. Feed Sci. Technol. 62 1996 203 213
91. B.S. Ramakrishna, S.H. Nance, I.C. Roberts-Thomson, and W.E.W. Roediger The effects of enterotoxins and shortchain fatty acids on water and electrolyte fluxes in ileal and colonic loops in vivo in the rat Digestion 45 1990 93 101
92. W.E.W. Roediger Role of anaerobic bacteria in the metabolic welfare of the colonic mucosa in man Gut 21 1980 793 798
93. W.E.W. Roediger Short chain fatty acids as metabolic regulators of ion absorption in the colon Acta Vet. Scand. 86 1989 116 125
94. C.J. Rumney, and I.R. Rowland In vivo and in vitro models of the human colonic flora Crit. Rev. Food Sci. Nutr. 31 1992 299 331
95. V. Salvador, C. Cherbut, J.L. Barry, D. Bertrand, C. Bonnet, and J. Delort-Laval Sugar composition of dietary fibre and short-chain fatty acid production during in vitro fermentation by human bacteria Br. J. Nutr. 70 1993 189 197
96. K.R. Silvester, H.N. Englyst, and J.H. Cummings Ileal recovery of starch from whole diets containing resistant starch measured in vitro and fermentation of ileal effluent Am. J. Clin. Nutr. 62 1995 403 411
97. M.R. Smiricky-Tjardes, E.A. Flickinger, C.M. Grieshop, L.L. Bauer, M.R. Murphy, and G.C. Fahey Jr. In vitro fermentation characteristics of selected oligosaccharides by swine fecal microflora J. Anim. Sci. 81 2003 2505 2514
98. M.R. Smiricky-Tjardes, C.M. Grieshop, E.A. Flickinger, L.L. Bauer, and G.C. Fahey Jr. Dietary galactooligosaccharides affect ileal and total-tract nutrient digestibility, ileal and fecal bacterial concentrations, and ileal fermentative characteristics of growing pigs J. Anim. Sci. 81 2003 2535 2545
99. M. Spanghero, and L.A. Volpelli A comparison of the predictions of digestible energy content of compound feeds for pigs by chemical or in vitro analysis Anim. Feed Sci. Technol. 81 1999 151 159
100. A. Stevenson, C.J. Buchanan, R. Abia, and M.A. Eastwood A simple in vitro fermentation system for polysaccharides - the effects of fermenter fluid surface area/fluid volume ratio and amount of substrate J. Sci. Food Agric. 73 1997 101 105
101. G.D. Sunvold, H.S. Hussein, G.C. Fahey Jr., N.R. Merchen, and G.A. Reinhart In vitro fermentation of cellulose, beet pulp, citrus pulp and citrus pectin using fecal inoculum from cats, dogs, horses and pigs and ruminal fluid from cattle J. Anim. Sci. 73 1995 3639 3648
102. M.K. Theodorou, B.A. Williams, M.S. Dhanoa, A.B. McAllan, and J. France A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feedstuffs Anim. Feed Sci. Technol. 48 1994 185 197
103. J.M.A. Tilley, and R.A. Terry A two-stage technique for the in vitro digestion of forage crops J. Br. Grassland Soc. 18 1963 104 111
104. E.C. Titgemeyer, L.D. Bourquin, G.C. Fahey Jr., and K.A. Garleb Fermentability of various fiber sources by human fecal bacteria in vitro Am. J. Clin. Nutr. 53 1991 1418 1424
105. J.M. Van der Meer, and J.M. Perez In vitro evaluation of European diets for pigs. Prediction of the organic matter digestibility by an enzymic method or by chemical analysis J. Sci. Food Agric. 59 1992 359 363
106. T. Van Kempen, S. Peak, and Y. Qiao In vitro digestibility could meet quality control needs. Feedstuffs 23 February 2004 11 13
107. H. Van Laar, S. Tamminga, B.A. Williams, and M.W.A. Verstegen Fermentation of the endosperm cells walls of monocotyledon and dicotyledon plant species by faecal microbes from pigs. The relationship between cell wall characteristics and fermentability Anim. Feed Sci. Technol. 88 2000 13 30
108. H. Van Laar, S. Tamminga, B.A. Williams, M.W.A. Verstegen, and H.A. Schols Fermentation characteristics of polysaccharide fractions extracted from the cell walls of maize endosperm J. Sci. Food. Agric. 82 2002 1369 1375
109. J. Van Loo, J. Cummings, N. Delzenne, H. Englyst, A. Franck, M. Hopkins, N. Kok, G.H. Macfarlane, D. Newton, M. Quigley, M. Roberfroid, T. van Vliet, and E. van den Heuvel Functional food properties of non-digestible oligosaccharides: a consensus report from the ENDO project Br. J. Nutr. 81 1999 121 132
110. I.J. Vervaeke, J.A. Decuypere, N.A. Dierick, and H.K. Henderickx Quantitative in vitro evaluation of the energy metabolism influenced by virginiamycin and spiramycin used as growth promoters in pig nutrition J. Anim. Sci. 49 1979 846 856
111. I.J. Vervaeke, N.A. Dierick, D.I. Demeyer, and J.A. Decuypere Approach to the energetic importance of fibre digestion in pigs. II. An experimental approach to hindgut digestion Anim. Feed Sci. Technol. 23 1989 169 194
112. A.J. Vince, P.F. Down, J. Murison, F.J. Twigg, and O.M. Wrong Generation of ammonia from non-urea sources in a faecal incubation system Gastroenterology 74 1976 313 322
113. A.J. Vince, N.I. McNeil, J.D. Wager, and O.M. Wrong The effect of lactulose, pectin, arabinogalactan and cellulose on the production of organic acids and metabolism of ammonia by intestinal bacteria in a faecal incubation system Br. J. Nutr. 63 1990 17 26
114. J.F. Wang, Y.H. Zhu, D.F. Li, Z. Wang, and B.B. Jensen In vitro fermentation of various fiber and starch sources by pig fecal inocula J. Anim. Sci. 82 2004 2615 2622
115. B.A. Williams, L.J.M. Van Osch, and R.P. Kwakkel Fermentation characteristics of the caecal contents of broiler chickens fed fine- and coarse particle diets Br. Poult. Sci. 38 1997 S41 S42
116. B.A. Williams, M. Bosch, J. Houdijk, and Y. van de Camp Differences in potential fermentative capabilities of four sections of porcine digestive tract. Proceedings of the 48th EAAP meeting Vienna, Austria 1997 Wageningen Pers Wageningen, The Netherlands 195
117. B.A. Williams, C. Voigt, and M. Verstegen The faecal microbial population can be representative of large intestinal microfloral activity Proceedings of the British Society of Animal Science Scarborough, UK 1998 165
118. B.A. Williams, S. Tamminga, and M.W.A. Verstegen Fermentation kinetics to assess microbial activity of gastrointestinal microflora Proceedings of the Symposium on Gas Production: Fermentation Kinetics for Feed Evaluation and to Assess Microbial Activity Wageningen, Netherlands 2000 British Society of Animal Sciences Penicuik, UK 97 100
119. B.A. Williams, M.W.A. Verstegen, and S. Tamminga Fermentation in the large intestine of single-stomached animals and its relationship to animal health Nutr. Res. Rev. 14 2001 207 227
120. B.A. Williams, M.W. Bosch, R. Noteborn, and M.W.A. Verstegen Changes in GIT digesta VFA as a result of fermentable carbohydrates in piglet diets Proceedings of the British Society of Animal Science Winter Meeting Scarborough, UK 2001 20
121. 3 concentration related to fermentable carbohydrates in piglet diets. Proceedings of the British Society of Animal Science Winter Meeting Scarborough, UK 2001 21
122. B.A. Williams, A. Awati, M.W. Bosch, S.R. Konstantinov, and A.D.L. Akkermans The effect of dietary fermentable carbohydrates on lactic acid concentration in small intestinal digesta of piglets at ten days after weaning Proceedings of the British Society of Animal Science Winter Meeting York, UK 2003 17
123. E. Wisker, M. Daniel, G. Rave, and W. Feldheim Fermentation of non-starch polysaccharides in mixed diets and single fibre sources: comparative studies in human subjects and in vitro Br. J. Nutr. 80 1998 253 261
124. E. Wisker, M. Daniel, G. Rave, and W. Feldheim Short-chain fatty acids produced in vitro from fibre residues obtained from mixed diets containing different breads and in human faeces during the ingestion of the diets Br. J. Nutr. 84 2000 31 37
125. J.T. Yen, J.A. Nienaber, D.A. Hill, and W.G. Pond Potential contribution of absorbed volatile fatty acids to whole-animal energy requirements in conscious swine J. Anim. Sci. 69 1991 2001 2012