Inhibition of Tat activity by the HEXIM1 protein

Retrovirology

Volumn 2, Issue , 2005, Pages

  Fraldi, Alessandro ^a,c   Varrone, Francesca ^a   Napolitano, Giuliana ^a   Michels, Annemieke A ^b   Majello, Barbara ^a   Bensaude, Olivier ^b   Lania, Luigi ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b ECOLE NORMALE SUPÉRIEURE   (France)

^c TELETHON INSTITUTE OF GENETICS AND MEDICINE TIGEM   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; CYCLIN DEPENDENT KINASE 9; CYCLIN T1; HEXIM1 PROTEIN; REGULATOR PROTEIN; RNA POLYMERASE II; SMALL NUCLEAR RNA; TRANSACTIVATOR PROTEIN; TRANSCRIPTION ELONGATION FACTOR; TRANSCRIPTION ELONGATION FACTOR B; TRANSCRIPTION FACTOR GAL4; UNCLASSIFIED DRUG; HEXIM1 PROTEIN, HUMAN; POSITIVE TRANSCRIPTION ELONGATION FACTOR B; RNA BINDING PROTEIN;

5' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; IN VITRO STUDY; IN VIVO STUDY; LONG TERMINAL REPEAT; MOLECULAR RECOGNITION; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN QUATERNARY STRUCTURE; TRANSACTIVATION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION; ANIMAL; CELL LINE; CHO CELL; DRUG ANTAGONISM; GENE EXPRESSION REGULATION; GENETICS; HAMSTER; METABOLISM; PROTEIN BINDING;

ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS 1;

ANIMALS; CELL LINE; CHO CELLS; CRICETINAE; GENE EXPRESSION REGULATION, VIRAL; GENE PRODUCTS, TAT; HUMANS; POSITIVE TRANSCRIPTIONAL ELONGATION FACTOR B; PROTEIN BINDING; RNA, SMALL NUCLEAR; RNA-BINDING PROTEINS;

EID: 27344447116     PISSN: 17424690     EISSN: 17424690     Source Type: Journal    
DOI: 10.1186/1742-4690-2-42     Document Type: Article

References (25)

1. Price DH: P-TEFb, a cyclin-dependent kinase controlling elongation by RNA polymerase II. Mol Cell Biol 2000, 20:2629-2634.
2. Napolitano G, Majello B, Lania L: Role of cyclinT/Cdk9 complex in basal and regulated transcription (Review). Int J Oncol 2002, 21:171-177.
3. Garriga J, Grana X: Cellular control of gene expression by T-type cyclin/CDK9 complexes. Gene 2004, 337:15-23.
4. Cullen BR: Does HIV-1 Tat induce a change in viral initiation rights? Cell 1993, 73:417-420.
5. Jones KA, Peterlin BM: Control of RNA initiation and elongation at the HIV-1 promoter. Ann Rev Biochem 1994, 63:717-743.
6. Brigati C, Giacca M, Noonan DM, Albini A: HIV Tat, its TARgets and the control of viral gene expression. FEMS Microbiol Lett 2003, 220:57-65.
7. Mancebo HS, Lee G, Flygare J, Tomassini J, Luu P, Zhu Y, Peng J, Blau C, Hazuda D, Price D, Flores O: P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro. Genes Dev 1997, 11:2633-2644.
8. Zhu Y, Pe'ery T, Peng J, Ramanathan Y, Marshall N, Marshall T, Amendt B, Mathews MB, Price DH: Transcription elongation factor P-TEFb is required for HIV-1 Tat transactivation in vitro. Genes Dev 1997, 11:2622-2632.
9. Bieniasz PD, Grdina TA, Bogerd HP, Cullen BR: Recruitment of a protein complex containing Tat and cyclin T1 to TAR governs the species specifity of HIV-1 Tat. EMBO J 1998, 17:7056-7065.
10. Garber ME, Wei P, KewalRamani VN, Mayall TP, Herrmann CH, Rice AP, Littman DR, Jones KA: The interaction beween HIV-1 Tat and human cyclin T1 requires zinc and a critical cysteine residue that is not conserved in the murine CycT1 protein. Genes Dev 1998, 12:3512-3527.
11. Nguyen VT, Kiss T, Michels AA, Bensaude O: 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes. Nature 2001, 414:322-325.
12. Yang Z, Zhu Q, Luo K, Zhou Q: The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription. Nature 2001, 414:317-322.
13. Michels AA, Nguyen VT, Fraldi A, et al.: MAQ1 and 7SK RNA interact with CDK9/cyclin T complexes in a transcription-dependent manner. Mol Cell Biol 2003, 23:4859-4869.
14. Yik JH, Chen R, Nishimura R, Jennings JL, Link AJ, Zhou Q: Inhibition of P-TEFb (CDK9/Cyclin T) kinase and RNA polymerase II transcription by the coordinated actions of HEXIM1 and 7SK snRNA. Mol Cell 2003, 12:971-982.
15. Michels AA, Fraldi A, Li Q, et al.: Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (CDK9/cyclin T) inhibitor. Embo J 2004, 23:2608-2619.
16. Chen R, Yang Z, Zhou Q: Phosphorylated positive transcription elongation factor b (P-TEFb) is tagged for inhibition through association with 7SK snRNA. J Biol Chem 2004, 279:4153-4160.
17. Yik JH, Chen R, Pezda AC, Samford CS, Zhou Q: A human immunodeficiency virus type 1 Tat-like arginine-rich RNA-binding domain is essential for HEXIM1 to inhibit RNA polymerase II transcription through 7SK snRNA-mediated inactivation of P-TEFb. Mol Cell Biol 2004, 24:5094-5105.
18. Byers SA, Price JP, Cooper JJ, Li Q, Price DH: HEXIM2, A HEXIM1 related protein, regulates P-TEFb through association with 7SK. J Biol Chem 2005, 280:16360-16367.
19. Yik JH, Chen R, Pezda AC, Zhou Q: Compensatory contributions of HEXIM1 and HEXIM2 in maintaining the balance of active and inactive P-TEFb complexes for control of transcription. J Biol Chem 2005, 280:16368-16376.
20. Majello B, Napolitano G, Giordano A, Lania L: Transcriptional regulation by targeted recruitment of cyclin-dependent CDK9 kinase in vivo. Oncogene 1999, 18:4598-4605.
21. Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM: Interaction between P-TEFb and the C-terminal domain of RNA polymerase II activates transcriptional elongation from sites upstream or downstream of target genes. Mol Cell Biol 2002, 22:321-331.
22. Murphy S, Yoon JB, Gerster T, Roeder RG: Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes. Mol Cell Biol 1992, 12:3247-3261.
23. Luecke HF, Yamamoto KR: The glucocorticoid receptor blocks P-TEFb recruitment by NFkappaB to effect promoter-specific transcriptional repression. Genes Dev 2005, 19:1116-1127.
24. Sano M, Wang SC, Shirai M, et al: Activation of cardiac Cdk9 represses PGC-1 and confers a predisposition to heart failure. Embo J 2004, 23:3559-3569.
25. Schulte A, Czudnochowski N, Barboric M, et al.: Identification of a cyclin T-binding domain in Hexim1 and biochemical analysis of its binding competition with HIV-1 Tat. J Biol Chem 2005, 280:24077-24968.