Excision of the epothilone synthetase B cyclization domain and demonstration of in trans condensation/cyclodehydration activity

Biochemistry

Volumn 44, Issue 40, 2005, Pages 13385-13393

  Kelly, Wendy L ^a   Hillson, Nathan J ^a   Walsh, Christopher T ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CONDENSATION; DEHYDRATION; ENZYME KINETICS; POLYPEPTIDES;

ASSEMBLY LINE; CYCLODEHYDRATION; EPOTHILONES; KINETIC CONSTANTS; NATURAL PRODUCTS;

ENZYMES;

ANTINEOPLASTIC AGENT; CARRIER PROTEIN; EPOTHILONE A; EPOTHILONE B; EPOTHILONE D; EPOTHILONE F; EPOTHILONE SYNTHETASE B; HETEROCYCLIC COMPOUND; NATURAL PRODUCT; NONRIBOSOMAL PEPTIDE SYNTHETASE; PEPTIDE SYNTHASE; POLYKETIDE SYNTHASE; SYNTHETASE; THIAZOLE DERIVATIVE; UNCLASSIFIED DRUG;

ADENYLATION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CYCLIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; OXIDATION; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CODON; DNA PRIMERS; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; KINETICS; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE SYNTHASES; POLYKETIDE SYNTHASES; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; TIME FACTORS;

EID: 26444617553     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051124x     Document Type: Article

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