Fluorescence lifetime imaging in an optically sectioning programmable array microscope (PAM)

Cytometry Part A

Volumn 67, Issue 2, 2005, Pages 112-118

  Hanley, Quentin S ^a,c   Lidke, Keith A ^a   Heintzmann, Rainer ^a,b   Arndt Jovin, Donna J ^a   Jovin, Thomas M ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b KING'S COLLEGE LONDON   (United Kingdom)

^c NOTTINGHAM TRENT UNIVERSITY   (United Kingdom)

Author keywords

AB plot; Frequency domain lifetime imaging; Structured illumination

Indexed keywords

ARTICLE; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; ILLUMINATION; IMAGE QUALITY; INSTRUMENT; LIGHT; MICROSCOPE; PRIORITY JOURNAL; PROGRAMMABLE ARRAY MICROSCOPE; SIMULATION;

ANIMALS; DNA-BINDING PROTEINS; DROSOPHILA PROTEINS; ENERGY TRANSFER; FLUORESCENCE; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY;

EID: 26444598606     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.20177     Document Type: Article

References (46)

1. Suhling K, French PM, Phillips D. Time-resolved fluorescence microscopy. Photochem Photobiol Sci 2005;4:13-22.
2. Jares-Erijman EA, Jovin TM. FRET imaging. Nat Biotechnol 2003;21: 1387-1395.
3. Buurman EP, Sanders R, Draaijer A, Gerritsen HC, Vanveen JJF, Houpt PM, Levine YK. Fluorescence lifetime imaging using a confocal laser scanning microscope. Scanning 1992;14:155-159.
4. Carlsson K, Liljeborg A. Simultaneous confocal lifetime imaging of multiple fluorophores using the intensity-modulated multiple-wavelength scanning (IMS) technique. J Microsc 1998;191:119-127.
5. French T, So PTC, Weaver DJ, Coelho-Sampaio T, Gratton E, Voss EW, Carrero J. Two-photon fluorescence lifetime imaging microscopy of macrophage-mediated antigen processing. J Microsc 1997;185:339-353.
6. Gerritsen HC, Vroom JM, de Grauw CJ. Combining two-photon excitation with fluorescence lifetime imaging. IEEE Eng Med Biol Mag 1999;18:31-36.
7. So PTC, Yu WN, Berland K, Dong CY, Gratton E. Time-resolved fluorescence microscopy using two-photon excitation. Bioimaging 1995;3: 1-15.
8. Ulrich V, Fischer P, Riemann I, Konig K. Compact multiphoton/single photon laser scanning microscope for spectral imaging and fluorescence lifetime imaging. Scanning 2004;26:217-225.
9. Agronskaia AV, Tertoolen L, Gerritsen HC. High frame rate fluorescence lifetime imaging. J Phys D 2003;36:1655-1662.
10. Gerritsen HC, Asselbergs MA, Agronskaia AV, Van Sark WG. Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution. J Microsc 2002;206:218-224.
11. Booth MJ, Wilson T. Low-cost, frequency-domain, fluorescence lifetime confocal microscopy. J Microsc 2004;214:36-42.
12. Van Munster EB, Gadella TWJ. φFLIM: a new method to avoid aliasing in frequency-domain fluorescence lifetime imaging microscopy. J Microsc 2004;213:29-38.
13. Bunt G, Wouters F. Visualization of molecular activities inside living cells with fluorescent labels. Rev Cytol 2004;237:205-277.
14. Fukano T, Miyawaki A. Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples. Appl Opt 2003;42:4119-4124.
15. Heintzmann R, Hanley QS, Arndt-Jovin DJ, Jovin TM. A dual path programmable array microscope (PAM): simultaneous acquisition of conjugate and non-conjugate images. J Microsc 2001;204:119-135.
16. Liang M, Stehr RL, Krause AW. Confocal pattern period in multiple-aperture confocal imaging systems with coherent illumination. Opt Lett 1997;22:751-753.
17. MacAulay CE, Dlugan ALP, Lane PM. Digital micromirror devices (DMDs) in microscopic and macroscopic imaging. In:Biomedical topical meetings. Volume SUG4-1. Washington, DC: Optical Society of America; 2000. p 117-119.
18. Hanley QS, Verveer PJ, Gemkow MJ, Arndt-Jovin DJ, Jovin TM. An optical sectioning programmable array microscope implemented with a digital micromirror device. J Microsc 1999;196:317-331.
19. Smith PJ, Taylor CM, Shaw AJ, McCabe EM. Programmable array microscopy with a ferroelectric liquid- crystal spatial light modulator. Appl Opt 2000;39:2664-2669.
20. Hanley QS, Verveer PJ, Jovin TM. Spectral imaging in a programmable array microscope by Hadamard transform fluorescence spectroscopy. Appl Spectrosc 1999;53:1-10.
21. Hanley QS, Verveer PJ, Jovin TM. Optical sectioning fluorescence spectroscopy in a programmable array microscope. Appl Spectrosc 1998;52:783-789.
22. Fulwyler MJ, Hanley QS, Schneller C, Young IT, Jares-Erijman EA, Arndi-Jovin DJ, Jovin TM. Selective photoreactions in a programmable array microscope (PAM): photoinitiated polymerization, photodecaging and photochromic conversion. Cytometry 2005. This issue.
23. Verveer PJ, Hanley QS, Verbeek PW, van Vliet LJ, Jovin TM. Theory of confocal fluorescence imaging in the programmable array microscope (PAM). J Microsc 1998;3:192-198.
24. Hanley QS, Verveer PJ, Arndt-Jovin DJ, Jovin TM. Three-dimensional spectral imaging by Hadamard transform spectroscopy in a programmable array microscope. J Microsc 2000;197:5-14.
25. Hanley QS, Jovin TM. Highly multiplexed optically sectioned spectroscopic imaging in a programmable array microscope. Appl Spectrosc 2001;55:1115-1123.
26. Hanley QS, Arndt-Jovin DJ, Jovin TM. Spectrally resolved fluorescence lifetime imaging microscopy. Appl Spectrosc 2002;56:155-166.
27. Lakowicz JR. Principles of fluorescence spectroscopy. 2nd ed. New York: Kluwer Academic/Plenum Publishers; 1999. p 142-184.
28. Marriott G, Parker I, editors. Biophotonics, part A. Methods in Enzymology, Volume 360. San Diego: Academic Press/Amsterdam: Elsevier; 2003.
29. Marriott G, Parker I, editors. Biophotonics, part B. Methods in Enzymology, Volume 361. San Diego: Academic Press/Amsterdam: Elsevier; 2003.
30. Valeur B. Molecular fluorescence: principles and applications. Weinheim/New York:Wiley-VCH; 2002.
31. Philip J, Carlsson K. Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging. J Opt Soc Am A 2003;20:368-379.
32. Verveer PJ, Squire A, Bastiaens PIH. Global analysis of fluorescence lifetime imaging microscopy data. Biophys J 2000;78:2127-2137.
33. Verveer PJ, Squire A, Bastiaens PIH. Improved spatial discrimination of protein reaction states in cells by global analysis and deconvolution of fluorescence lifetime imaging microscopy data. J Microsc 2001; 202:451-456.
34. Verveer PJ, Bastiaens PIH. Evaluation of global analysis algorithms for single frequency fluorescence lifetime imaging microscopy data. J Microsc 2003;209:1-7.
35. Treanor B, Lanigan PM, Suhling K, Schreiber T, Munro I, Neil MA, Phillips D, Davis DM, French PM. Imaging fluorescence lifetime heterogeneity applied to GFP-tagged MHC protein at an immunological synapse. J Microsc 2005;217:36-43.
36. Grecco HE, Lidke KA, Heintzmann R, Lidke DS, Spagnuolo C, Martinez OE, Jares-Erijman EA, Jovin TM. Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells. Microsc Res Tech 2004;65:169-179.
37. Pepperkok R, Squire A, Geley S, Bastiaens PIH. Simultaneous detection of multiple green fluorescent proteins in live cells by fluorescence lifetime imaging microscopy. Curr Biol 1999;9:269-272.
38. Harpur AG, Wouters FS, Bastiaens PIH. Imaging FRET between spectrally similar GFP molecules in single cells. Nat Biotechnol 2001;19: 167-169.
39. Subramaniam V, Hanley QS, Clayton AHA, Jovin TM. Photophysics of green and red fluorescent proteins: some implications for quantitative microscopy. Methods Enzymol 2003;360:178-201.
40. Clayton AHA, Hanley QS, Verveer PJ. Graphical representation and multicomponent analysis of single-frequency fluorescence lifetime imaging microscopy data. J Microsc 2004;213:1-5.
41. Hanley QS, Clayton AHA. AB-plot assisted determination of fluorophore mixtures in a fluorescence lifetime microscope using spectra or quenchers. J Microsc 2005;218:62-67.
42. Sokal RR, Rohlf FJ. Biometry: the principles and practice of statistics in biological research. New York: Freeman; 2001.
43. Hanley QS, Subramaniam V, Arndt-Jovin DJ, Jovin TM. Fluorescence lifetime imaging: multi-point calibration, minimum resolvable differences, and artifact suppression. Cytometry 2001;43:248-260.
44. van der Voort HTM, Brakenhoff GJ. 3-D image formation in high-aperture fluorescence confocal microscopy: a numerical analysis. J Microsc 1990;158:43-54.
45. Clayton AHA, Hanley QS, Arndt-Jovin DJ, Subramaniam V, Jovin TM. Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM). Biophys J 2002;83:1631-1649.
46. Neher R, Neher E. Optimizing imaging parameters for the separation of multiple labels in a fluorescence image. J Microsc 2004;213:46-62.